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Bio-activity of Algae Belonging to Bhusawal region, Maharashtra

Three algal species; Spirogyra (A), Chara (B) and Cladophora (C) were isolated from Tapi river near Bhusawal (M.S.). These algae were tested in compliance with the agar well diffusion method for their antibacterial and antifungal agent production on various organisms that incite diseases to human and plants (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, Asperigillus niger and Candida albicans). The bioactive metabolites are extracted by using diethyl ether, butanol, acetone and methanol. It was found that; sample A had the highest bioactivity towards the tested bacteria. Algae are found to be rich source nutrients like carbohydrates, lipids and proteins. Thus they act as growth promoting substance for fungi like Candia albicans and Asperigillus niger. From tested algae B has highest growth promoting ability for fungi Candia albicans and Asperigillus niger

Surface runoff estimation using geographic information system and soil conservation service-curve number method for sub catchments of Karamadai, Tamil Nadu

Water conservation becomes essential as the resource becomes scarcer. The most important step in managing water resources is estimating watershed runoff generated from rainfall, as the runoff and rainfall are the key factors in determining water availability for surface storage and groundwater recharge. So, this study is mainly focused on estimating the surface runoff generated from the three sub-catchments of Karamadai, Tamil Nadu, India, using the heavy to extreme daily rainfall events received in the study area within the span of 20 years (2000–2019). The study was performed in the ArcGIS environment using remote sensing data. The SCSCN (Soil Conservation Service-Curve Number) method was used to estimate surface runoff. The changes in the land use in each sub-catchment were analysed in each decade and studied for their impact on the runoff depth. The land use and land cover classification map of the study area was prepared from LISS III satellite imagery for the years 2006 and 2016 by using supervised classification. The curve number was assigned based on land use as well as the hydrologic soil group. The weighted curve number was calculated from the area under each land use and then used to calculate storm runoff. The maximum runoff occurred in 2011 in all the catchments of the Karamadai block. It was found that more runoff occurred in the Mandrai Pallam catchment compared to Periya Pallam and Pare Pallam, as the Mandrai Pallam catchment had less soil moisture retention capacity than the other two catchments. So, more priority must be given to this catchment while planning to implement the soil and water conservation measures

Mitochondrial DNA marker reveals shallow genetic structuring in Priacanthus hamrur (Forsskål, 1775) along the Indian coast

Priacanthus hamrur (Forsskål, 1775), a marine perch belonging to the family Priacanthidae commonly known as “bulls eye” has started emerging as an important fishery resource in the trawl landings along both the west and east coasts of India. In the present study, genetic stock structure of P. hamrur inhabiting Indian coastal waters was ascertained with mitochondrial DNA sequences from the cytochrome b (cyt-b) gene using samples of the species collected in two samplings from five different geographical locations, viz. Chennai and Visakhapatnam (east coast) and Cochin, Mumbai and Veraval (West coast). Partial sequence of cyt-b gene of P. hamrur from 5 representative regions along the coastal zones was amplified by PCR. The cyt- b marker revealed high haplotype diversity coupled with very low nucleotide diversity within each population, as well as low genetic distance, high gene flow, and high mitochondrial DNA similarity among all five populations. Phylogenetic trees and pairwise analyses demonstrated a very small divergence (0.43-0.64%) between the populations, suggesting the lack of population subdivisions. The overall lack of genetic subdivision among samples was also detected by the analysis of molecular variance, and pairwise FST values. Furthermore, the results of this study revealed a pattern of high nucleotide homology among the adjacent populations, and a small number of nucleotide changes among disjunct populations, leading us to conclude that there is a genetic connectivity among the populations of P.hamrur inhabiting the coastal waters of India

Manipulation of fatty acids in the estuarine clam Meretrix casta (Gmelin, 1791) by supplementation with the microalgal diet, Isochrysis galbana

The present study evaluated the changes in fatty acid profile of the estuarine clam Meretrix casta, an important food organism used in the larval rearing of scyllarid lobsters, after supplementation with the microalgal species Isochrysis galbana. The uptake and assimilation of lipids from the microalgal feed were verified by gas chromatographic analysis of fatty acids in the clam tissues after eight days of feeding with I. galbana. Increase in concentrations of polyunsaturated fatty acids (PUFA), in particular docosahexaenoic acid (DHA, C22:6n3) and C18:2n-6, was observed in clams supplemented with I. galbana. Changes in monounsaturated fatty acid (MUFA) composition were less marked and related to the increasing proportions of C18:1, after supplementation. Feeding with I. galbana also induced a decrease in the proportion of saturated fatty acids, which was related to decrease in proportions of both C16:0 and C18:0. Although the fatty acid composition showed significant differences, the gross lipid content of the clam tissues did not seem to be excessively influenced by the algal feeding. Tissues from clams supplemented with I. galbana are being evaluated as feed for sand lobster larval trials

Cardiovascular outcomes following a respiratory tract infection among adults with non-CF bronchiectasis: a general population based study

Rationale: Studies suggest that people with bronchiectasis are at increased risk of cardiovascular co-morbidities. Objectives: We aimed to quantify the relative risk of incident cardiovascular events following a respiratory tract infection amongst people with bronchiectasis. Methods: Using UK electronic primary care records, we conducted a within-person comparison using the self-controlled case series method. We calculated the relative risk of first time cardiovascular events (either first myocardial infarction [MI] or stroke) following a respiratory tract infection compared with the individual’s baseline risk. Results: Our cohort consisted of 895 individuals with non-CF bronchiectasis with a first MI or stroke and at least one respiratory tract infection. There was an increased rate of first time cardiovascular events in the 91 day period after a respiratory tract infection (Incidence Rate Ratio [IRR] 1.56; 95% CI 1.20 to 2.02). The rate of a first cardiovascular event was highest in the first three days following a respiratory tract infection (IRR 2.73, 95% CI 1.41 to 5.27). Conclusions: These data suggest that respiratory tract infections are strongly associated with a transient increased risk of first time MI or stroke amongst people with bronchiectasis. As respiratory tract infections are six times more common in people with bronchiectasis than the general population, the increased risk has a disproportionately greater impact in these individuals. These findings may have implications for including cardiovascular risk modifications in airway infection treatment pathways in this population

Omega Production in pp Collisions

A model-independent irreducible tensor formalism which has been developed earlier to analyze measurements of $\vec{p}\vec{p}\to pp \pi^\circ$, is extended to present a theoretical discussion of $\vec{p}\vec{p}\to pp \omega$ and the polarization of $\omega$ in $pp\to pp \vec{\omega}$. The recent measurement of unpolarized differential cross section for $pp\to pp \omega$ is analyzed using this theoretical formalism.Comment: 5 pages (double column), no figures, uses revtex

Involvement of a periplasmic protein kinase in DNA strand break repair and homologous recombination in Escherichia coli

The involvement of signal transduction in the repair of radiation-induced damage to DNA has been known in eukaryotes but remains understudied in bacteria. This article for the first time demonstrates a role for the periplasmic lipoprotein (YfgL) with protein kinase activity transducing a signal for DNA strand break repair in Escherichia coli. Purified YfgL protein showed physical as well as functional interaction with pyrroloquinoline-quinone in solution; the protein kinase activity of YfgL was strongly stimulated in the presence of pyrroloquinoline-quinone. Transgenic E. coli cells producing Deinococcus radiodurans pyrroloquinoline-quinone synthase showed nearly four log cycle improvement in UVC dark survival, 0-fold increases in gamma radiation resistance as compared with untransformed cells. Pyrroloquinoline-quinone enhanced the UV resistance of E. coli through the YfgL protein; required the active recombination repair proteins. The yfgL mutant showed higher sensitivity to UVC, mitomycin C, gamma radiation as compared with wild-type cells, showed a strong impairment in homologous DNA recombination. The mutant expressing an active YfgL in trans recovered the lost phenotypes to nearly wild-type levels. The results strongly suggest that the periplasmic phosphoquinolipoprotein kinase YfgL plays an important role in radiation-induced DNA strand break repair, homologous recombination in E. coli

A STUDY ON THE DEVELOPMENT AND SANITATION PROFILE OF SMART CITIES – A CASE STUDY OF TIRUCHIRAPALLI CITY

Health and Sanitation are the two major limbs that support Swachh Bharat Abhiyaan. This scheme was launched by the Indian Government targeting towards achieving a more clean and healthy India. The present study was done for estimating the cleanliness and development of Tiruchirapalli city located in the state of Tamil Nadu. Geospatial & Statistical tools were used for analyzing and ranking different wards. Panchromatic & MSS merged image and ancillary data obtained from Trichy Municipal Corporation was used to derive necessary GIS layers. Adequacy of the public toilets for each ward was found out using spatial layers and mathematical equation derived from census and sanitation data. Development Index was generated for each ward giving appropriate ranking by considering parameters such as public toilets, population, built-up densities and NDVI for each ward. The results were used for estimating the adequacy of public toilets in each ward and deciding the best wards based on ranks obtained from weighted values of different developmental parameters

A Study to Analyze Different Patterns of Quid Usage among Subjects with Oral Submucous Fibrosis in Mangalore Population

Aim and Objectives. Oral submucous fibrosis (OSF) is a potentially malignant disorder associated with the usage of areca nut. Usage of processed forms of areca nut is popular among the youth and its carcinogenic effects are not well known. Due to large immigrant population, various patterns of areca nut usage are seen. The aim of this study is to assess the various quid chewing patterns and their association with severity of OSF. Materials and Methods. A cross-sectional study was carried out with 250 cases clinically and histologically diagnosed as having OSF lesion that were selected and subjected to a detailed habit history which was recorded through preformed questionnaire. The data obtained was statistically analyzed. Results. Among the 250 subjects, males were seen to be affected more than females within the age group of 26-35 years and were having clinical stage I OSF. A combination of processed areca nut and processed tobacco was used by the majority of the subjects with duration of 1 to 5 years, at a frequency of 3 to 5 quids per day. Conclusion. The present study confirms the association between oral submucous fibrosis and the quid containing processed areca nut and processed tobacco and also highlights the increasing youth population using the processed forms of areca nut