Differential expression of the brassinosteroid receptor-encoding BRI1 gene in Arabidopsis

Abstract Brassinosteroid (BR)-regulated growth and development in Arabidopsis depends on BRASSINOSTEROID INSENSITIVE 1 (BRI1), the BR receptor that is responsible for initiating the events of BR signalling. We analysed the temporal and spatial regulation of BRI1 expression using stable transgenic lines that carried BRI1 promoter:reporter fusions. In both seedlings and mature plants the tissues undergoing elongation or differentiation showed elevated BRI1 gene activity, and it could be demonstrated that in the hypocotyl this was accompanied by accumulation of the BRI1 transcript and its receptor protein product. In seedlings the BRI1 promoter was also found to be under diurnal regulation, determined primarily by light repression and a superimposed circadian control. To determine the functional importance of transcriptional regulation we complemented the severely BR insensitive bri1-101 mutant with a BRI1-luciferase fusion construct that was driven by promoters with contrasting specificities. Whereas the BRI1 promoter-driven transgene fully restored the wild phenotype, expression from the photosynthesisassociated CAB3 and the vasculature-specific SUC2 and ATHB8 promoters resulted in plants with varying morphogenic defects. Our results reveal complex differential regulation of BRI1 expression, and suggest that by influencing the distribution and abundance of the receptor this regulation can enhance or attenuate BR signalling

Explosion areas of flammable substances and their numerical approximation

CHEMSAFE® database [1], developed as a joint project between BAM, PTB and DECHEMA, contains safety characteristics of flammable liquids, gases, dusts and their mixtures. The BAM is responsible for evaluated data on gases and dusts. TRIANGLE software, originally developed by the BAM for the processing, quality assurance and visualisation of measured data describing the explosion area of ternary systems consisting of flammable, inert and oxidising gases. 2D figures in the program represent isothermal or isobaric level curves of the explosion areas. In order to better describe the limiting curve of the explosion range, by the BUTE (Budapest University of Technolcgy and Economics) the Akima- spline interpolation has been replaced by special comonoton Splines in TRIANGLE. For the 3D visualisation of the whole temperature- or pressure-dependent ternary explosion surfaces the TRIGON program of BUTE was extended

Esigenze abitative ed istanze di conservazione nel recupero dell'architettura esistente: il quartiere INA casa La Loggetta, Napoli, 1954-1994

Dottorato di ricerca in recupero edilizio e ambientale. 8. ciclo. A.a. 1992-95. Tutori G. Caterina e B. GabrielliConsiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - P.za Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal