On the influence of collinear surface waves on turbulence in smooth-bed open-channel flows

This work investigates how turbulence in open-channel flows is altered by the passage of surface waves by using experimental data collected with laboratory tests in a large-scale flume facility, wherein waves followed a current. Flow velocity data were measured with a laser Doppler anemometer and used to compute profiles of mean velocity and Reynolds stresses, and pre-multiplied spectra. The velocity signal containing contributions from the mean flow, wave motion and turbulence was decomposed using the empirical mode decomposition (EMD), which is considered a promising tool for the analysis of velocity time series measured in complex flows. A novel outer length scale h0 is proposed which separates the flow into two regions depending on the competition between the vertical velocities associated with the wave motion and the turbulent velocities imposed by the current. This outer length scale allows for the identification of a genuine overlap layer and an insightful scaling of turbulent statistics in the current-dominated flow region (i.e. y/h0 > 1), a new spectral signature associated with long turbulent structures (approximately 6 and 25 times the flow depth h). As the wave contribution to the vertical velocity increases, the pre-multiplied spectra reveal two intriguing features: (i) in the current-dominated flow region, the very large-scale motions (VLSMs) are progressively weakened but attached eddies are still present; and (ii) in the wave-dominated flow region (i.e. 1$]) appears. These longitudinal structures present in the wave-dominated flow region seem to share many features with Langumir-type cells

Optical mapping of neuronal activity during seizures in zebrafish

Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs. Recently, zebrafish has become an important model for studying epilepsy both in basic research and in drug discovery. Here, we employed a transgenic line with pan-neuronal expression of the genetically-encoded calcium indicator GCaMP6s to measure neuronal activity in zebrafish larvae during seizures induced by pentylenetretrazole (PTZ). With this approach, we mapped neuronal activity in different areas of the larval brain, demonstrating the high sensitivity of this method to different levels of alteration, as induced by increasing PTZ concentrations, and the rescuing effect of an anti-epileptic drug. We also present simultaneous measurements of brain and locomotor activity, as well as a high-throughput assay, demonstrating that GCaMP measurements can complement behavioural assays for the detection of subclinical epileptic seizures, thus enabling future investigations on human hypomorphic mutations and more effective drug screening methods. Notably, the methodology described here can be easily applied to the study of many human neuropathologies modelled in zebrafish, allowing a simple and yet detailed investigation of brain activity alterations associated with the pathological phenotype

Lyapunov Control on Quantum Open System in Decoherence-free Subspaces

A scheme to drive and manipulate a finite-dimensional quantum system in the decoherence-free subspaces(DFS) by Lyapunov control is proposed. Control fields are established by Lyapunov function. This proposal can drive the open quantum system into the DFS and manipulate it to any desired eigenstate of the free Hamiltonian. An example which consists of a four-level system with three long-lived states driven by two lasers is presented to exemplify the scheme. We have performed numerical simulations for the dynamics of the four-level system, which show that the scheme works good.Comment: 5 pages, 6 figure

Feedback control of spin systems

The feedback stabilization problem for ensembles of coupled spin 1/2 systems is discussed from a control theoretic perspective. The noninvasive nature of the bulk measurement allows for a fully unitary and deterministic closed loop. The Lyapunov-based feedback design presented does not require spins that are selectively addressable. With this method, it is possible to obtain control inputs also for difficult tasks, like suppressing undesired couplings in identical spin systems.Comment: 16 pages, 15 figure

Autosomal Dominant Tubulointerstitial Kidney Disease with Adult Onset due to a Novel Renin Mutation Mapping in the Mature Protein

Autosomal dominant tubulointerstitial kidney disease (ADTKD) is a genetically heterogeneous renal disorder leading to progressive loss of renal function. ADTKD-REN is due to rare mutations in renin, all localized in the protein leader peptide and affecting its co-translational insertion in the endoplasmic reticulum (ER). Through exome sequencing in an adult-onset ADTKD family we identified a new renin variant, p.L381P, mapping in the mature protein. To assess its pathogenicity, we combined genetic data, computational and predictive analysis and functional studies. The L381P substitution affects an evolutionary conserved residue, co-segregates with renal disease, is not found in population databases and is predicted to be deleterious by in silico tools and by structural modelling. Expression of the L381P variant leads to its ER retention and induction of the Unfolded Protein Response in cell models and to defective pronephros development in zebrafish. Our work shows that REN mutations outside of renin leader peptide can cause ADTKD and delineates an adult form of ADTKD-REN, a condition which has usually its onset in childhood. This has implications for the molecular diagnosis and the estimated prevalence of the disease and points at ER homeostasis as a common pathway affected in ADTKD-REN, and possibly more generally in ADTKD

Percutaneous ethanol injection of hepatic tumors : single-session therapy with general anesthesia

OBJECTIVE: We studied the feasibility and the effectiveness of percutaneous ethanol injection, performed with general anesthesia in a single session, for treating malignant hepatic lesions. SUBJECTS AND METHODS: We treated 30 patients with sonographically guided percutaneous injection of ethanol. Twenty had hepatocellular carcinoma and cirrhosis, and 10 had hepatic metastases, principally from colon cancer. The mean volume of ethanol injected was 57 ml (range, 6-165 ml). RESULTS: CT showed complete necrosis (up to 8.2 cm) in seven of 10 patients with encapsulated hepatocellular carcinoma and about 90% necrosis in the remaining three patients. In four of these patients, the alpha-fetoprotein level fell from more than 200 ng/ml to less than 20 ng/ml during treatment. In 10 patients with infiltrating hepatocellular carcinoma, about 70-90% necrosis was achieved; in six of these patients, the alpha-fetoprotein level, which had been more than 200 ng/ml, decreased during treatment. In the 10 patients with metastases, more than 50% necrosis was always achieved. Levels of carcinoembryonic antigen decreased after treatment in all patients. In three patients who had cirrhosis with superficial hepatocellular carcinoma, peritoneal hemorrhage occurred but did not require transfusion. CONCLUSION: Our results show that percutaneous injection of ethanol in a single session with general anesthesia is feasible and effective and has several advantages over multisession therapy. These include shorter treatment time and the ability to treat larger and more numerous lesions