7 research outputs found
Vaccination of African catfish with Vibrio anguillarum O2: II. Oral intubation with vaccine-containing pellets
The aim of this study was to investigate the success of oral vaccination application in African catfish using Vibrio anguillarum O2 bacterins. The antigen uptake was followed by competitive enzyme-linked immunosorbent assay (ELISA). Serum antibody response was measured using an indirect ELISA. Several in vivo administration methods were investigated. Intraperitoneal injection gave the highest absorption rate, with high antibody levels in the systemic circulation. Oral intubation of bacterin-layered pellets resulted in low antigen uptake and low antibody levels. The addition of absorption enhancers increased the serum antigen levels. An enteric coating applied on the pellets containing vaccine did not improve the immune response.status: publishe
Vaccination of African catfish with Vibrio anguillarum O2: I. ELISA development and response to IP and immersion vaccination
The aim of this study was to investigate African catfish (Clarias gariepinus) vaccination with Vibrio anguillarum O2 bacterins. To monitor the antigen uptake, a competitive enzyme-linked immunosorbent assay (ELISA) was developed. Serum antibody response was measured using an indirect ELISA. Sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblot data that characterize the immunoglobulin molecule of the African catfish are presented. The impact of acid conditions on the antigen proved to be stable above pH 4. A partition coefficient was calculated to determine the ability of transepithelial uptake. Two in vivo administration methods were investigated in the study. Intraperitoneal injection gave the highest absorption rate with high antibody levels in the systemic circulation, whereas immersion did not induce significant serum antibody levels.status: publishe
CΡΠ°Π½Π΄Π°ΡΡΡΠ·ΠΎΠ²Π°Π½Π½ΠΈΠΉ Π΅ΠΊΡΡΡΠ°ΠΊΡ Ρ ΠΌΠ΅Π»Ρ Π² ΠΏΠΎΠ»Π΅Π³ΡΠ΅Π½Π½Ρ ΡΠΈΠΌΠΏΡΠΎΠΌΡΠ² ΠΌΠ΅Π½ΠΎΠΏΠ°ΡΠ·ΠΈ
ΠΠ½ΡΠ΅ΡΠ΅Ρ ΠΊ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΈΡΠΎΡΡΡΡΠΎΠ³Π΅Π½ΠΎΠ² ΠΏΡΠΈ Π²Π΅Π΄Π΅Π½ΠΈΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΊΠ»ΠΈΠΌΠ°ΠΊΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΆΠ°Π»ΠΎΠ±Π°ΠΌΠΈ Ρ ΠΊΠ°ΠΆΠ΄ΡΠΌ Π³ΠΎΠ΄ΠΎΠΌ Π²ΠΎΠ·ΡΠ°ΡΡΠ°Π΅Ρ. Π€ΠΈΡΠΎΡΡΡΡΠΎΠ³Π΅Π½Ρ β ΡΡΠΎ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ Π½Π΅ΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΡΠ΅ ΠΏΠΎΠ»ΠΈΡΠ΅Π½ΠΎΠ»ΡΠ½ΡΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ, ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎ ΠΈΠΌΠΈΡΠΈΡΡΡΡΠΈΠ΅ Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΡΡΠΎΠ³Π΅Π½Π° ΠΈ 17Ξ²-ΡΡΡΡΠ°Π΄ΠΈΠΎΠ»Π°. Π Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π½Π°ΡΡΡΠ΅Π½Π½ΡΠΌ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠ°ΠΌ ΡΠΈΡΠΎΡΡΡΡΠΎΠ³Π΅Π½ΠΎΠ² ΠΎΡΠ½ΠΎΡΡΡ ΡΠΎΡ ΠΈ ΠΊΡΠ°ΡΠ½ΡΠΉ ΠΊΠ»Π΅Π²Π΅Ρ (ΠΈΠ·ΠΎΡΠ»Π°Π²ΠΎΠ½Ρ), ΡΠ΅ΠΌΠ΅Π½Π° Π»ΡΠ½Π° (Π»ΠΈΠ³Π½Π°Π½Ρ) ΠΈ Ρ
ΠΌΠ΅Π»Ρ (ΠΏΡΠ΅Π½ΠΈΠ»ΡΠ»Π°Π²ΠΎΠ½ΠΎΠΈΠ΄Ρ) (Cos et al., 2003)
Hydrophilic excipients modulate the time lag of time-controlled disintegrating press-coated tablets
An oral press-coated tablet was developed by means of direct compression to achieve the time-controlled disintegrating or rupturing function with a distinct predetermined lag time. This press-coated tablet containing sodium diclofenac in the inner core was formulated with an outer shell by different weight ratios of hydrophobic polymer of micronized ethylcellulose (EC) powder and hydrophilic excipients such as spray-dried lactose (SDL) or hydroxypropyl methylcellulose (HPMC). The effect of the formulation of an outer shell comprising both hydrophobic polymer and hydrophilic excipients on the time lag of drug release was investigated. The release profile of the press-coated tablet exhibited a time period without drug release (time lag) followed by a rapid and complete release phase, in which the outer shell ruptured or broke into 2 halves. The lag phase was markedly dependent on the weight ratios of EC/SDL or EC/HPMC in the outer shell. Different time lags of the press-coated tablets from 1.0 to 16.3 hours could be modulated by changing the type and amount of the excipients. A semilogarithmic plot of the time lag of the tablet against the weight ratios of EC/SDL or EC/HPMC in the outer shell demonstrated a good linear relationship, withr=0.976 andr=0.982, respectively. The predetermined time lag prior to the drug release from a press-coated tablet prepared by using a micronized EC as a retarding coating shell can be adequately scheduled with the addition of hydrophilic excipients according to the time or site requirements