Antioxidant Activity of Silica-Based Bioactive Glasses

Bioactive glasses are the materials of choice in the field of bone regeneration. Antioxidant properties of interest to limit inflammation and foreign body reactions have been conferred to bioactive glasses by the addition of appropriate ions (such as Ce or Sr). On the other hand, the antioxidant activity of bioactive glasses without specific ion/molecular doping has been occasionally cited in the literature but never investigated in depth. In the present study, three silica-based bioactive glasses have been developed and characterized for their surface properties (wettability, zeta potential, chemical composition, and reactivity) and radical scavenging activity in the presence/absence of cells. For the first time, the antioxidant activity of simple silica-based (SiO2-CaO-Na2O) bioactive glasses has been demonstrated

Development of Super‐Paramagnetic Iron Oxide Nanoparticle‐Coated Melt Electrowritten Scaffolds for Biomedical Applications

Polycaprolactone (PCL) is usually the material chosen for melt electrowriting (MEW) due to its biocompatibility, mechanical strength, and melt processability. This work first investigates the effect of different processing parameters to obtain optimum PCL‐MEW scaffolds. Secondly, to increase PCL`s hydrophilicity and cell affinity, and to enable coating with superparamagnetic iron oxide nanoparticles (SPIONs) and silica‐coated‐SPIONs (Si‐SPIONs), the scaffolds are modified with alkaline surface treatment. Finally, SPIONs and Si‐SPIONs are successfully coated on MEW scaffolds. Results show that reproducible scaffolds are fabricated. Additionally, the alkaline treatment does not change the three‐dimensional morphology of scaffolds while reducing fiber diameter. Furthermore, SEM images and ATR‐FTIR results confirmed that SPIONs and Si‐SPIONs‐were coated on scaffolds. A cytocompatibility assay showed a non‐toxic effect on MG‐63 osteoblast‐like cells in all scaffolds. Additionally, higher MC3T3‐E1 pre‐osteoblastic cell adhesion efficiency and proliferation are achieved for the alkaline‐treated scaffolds and SPIONs/Si‐SPIONs‐coated scaffolds. All samples demonstrated the ability to generate heat, useful for hyperthermia‐treatment, when subjected to an alternating magnetic field. Overall, the findings suggest that the strategy of coating PCL‐MEW scaffolds with SPIONs/Si‐SPIONs has the potential to improve scaffold performance for biomedical applications, especially for producing magnetically responsive MEW scaffolds.Polycaprolactone is used to fabricate superparamagnetic iron oxide nanoparticle (SPION) and silica‐coated‐SPION (Si‐SPION)‐coated scaffolds by melt electrowriting (MEW). The scaffolds’ hydrophilicity is modified by alkaline treatment. Scaffolds exhibit non‐toxicity. High pre‐osteoblastic (MC3T3‐E1) cell adhesion and proliferation are achieved, while all scaffolds show the ability to generate heat, useful for hyperthermia treatment when subjected to an alternating magnetic field. image </p

Electrophoretic deposition of composite coatings based on alginate matrix/45S5 bioactive glass particles doped with B, Zn or Sr

In this research work composite coatings made of alginate and 45S5 bioactive glass particles doped with B, Zn or Sr were synthesized by means of electrophoretic deposition and characterized from morphological, compositional, thermogravimetric, mechanical and electrochemical points of view. The developed coatings were also subjected to in vitro test in SBF solution to evaluate their ability to induce hydroxyapatite precipitation and cytocompatibility evaluation using human primary fibroblasts. The obtained results demonstrated a good homogeneity of the coatings, high adhesion and a protective behavior towards the substrate. The thermogravimetric analysis proved that the glass amount was constant before and after the deposition and all the investigated coatings promoted the deposition of hydroxyapatite but with different kinetics. Since the Zn containing coating showed the best bioactive behavior it was subjected to cytocompatibility test, which demonstrated, after an initial reduction of cell viability, a good cell proliferation and the production of collagen from the ECM. These findings suggest that the obtained coatings are promising materials to coat metallic prosthetic devices

Surface modification of silicate, borosilicate and phosphate bioactive glasses to improve/control protein adsorption : PART I

Bioactive glasses (BGs) are promising for bone tissue regeneration. BG composition can be tailored, according to the application of interest, and/or functionalized with organic molecules/biomolecules to improve their performances. However, despite the wide knowledge concerning BGs, their interaction with proteins, fundamental for controlling the fate of the implant, has not been deeply investigated yet. Controlling or predicting protein adsorption requires a full understanding of the materials surface physico-chemical properties. In this work, four different BGs (S53P4, B25, SCNB, PhGlass) were surface-modified by four different treatments: 72 h-soaking in TRIS, 72 h soaking in simulated body fluid, APTES grafting and quaternized APTES grafting. The surfaces were then characterized both untreated and after each treatment by contact angle, zeta potential analysis, X-ray photoelectron spectroscopy, Fourier Transform InfraRed–Attenuated Total Reflectance spectroscopy and Scanning Electron Microscopy and Energy Dispersive Spectroscopy. Inductively Coupled Plasma – Optical Emission Spectrometry was then performed to investigate the ion leaching. The aim of this study (Part I) is the physico-chemical characterization of BGs as a function of the implemented treatments, aiming to better understand how the superficial properties are successively affecting protein adsorption. Protein adsorption on untreated and treated BGs will be discussed in a following manuscript (Part II).Peer reviewe

Surface modification of silicate, borosilicate, and phosphate bioactive glasses to improve/control protein adsorption : PART II

Bioactive glasses (BGs) are characterized by high biocompatibility and bioactivity and are particularly promising for bone tissue regeneration. Once implanted, the BGs interact with the environment and adsorb chemical moieties and biomolecules. Proteins in body fluids are critical for the success of implants, because the adsorption of specific proteins can either promote or inhibit the adhesion of surrounding tissue or other factors such as bacteria. Controlling protein adsorption by tailoring the surface properties of implanted biomaterials is fundamental. This can determine the fate of the implant. In the current study, four BG compositions (two silicates, one borosilicate, and one phosphate glass) and three model proteins (fibronectin, chimeric avidin, and streptavidin) were considered. Each BG was surface pretreated, and the adsorption of fluorescently labeled fibronectin, chimeric avidin, or streptavidin was monitored. Untreated surfaces were used as controls. The amount and spatial distribution of each protein were estimated by confocal microscopy in fluorescence modality, followed by protein clustering analysis. Although streptavidin was not adsorbed efficiently on any of the considered substrates, BGs were successfully coated with fibronectin and chimeric avidin. Both proteins showed different affinities and surface distributions as functions of the implemented pretreatment on each substrate.publishedVersionPeer reviewe

Iron Oxide-Au Magneto-Plasmonic Heterostructures: Advances in Their Eco-Friendly Synthesis

In recent years, nanotechnologies have attracted considerable interest, especially in the biomedical field. Among the most investigated particles, magnetic based on iron oxides and Au nanoparticles gained huge interest for their magnetic and plasmonic properties, respectively. These nanoparticles are usually produced starting from processes and reagents that can be the cause of potential human health and environmental concerns. For this reason, there is a need to develop simple, green, low-cost, and non-toxic synthesis methods and reagents. This review aims at providing an overview of the most recently developed processes to produce iron oxide magnetic nanoparticles, Au nanoparticles, and their magneto-plasmonic heterostructures using eco-friendly approaches, focusing the attention on the microorganisms and plant-assisted syntheses and showing the first results of the development of magneto-plasmonic heterostructures