Efficient photochemical activity and strong dichroism of single crystals of reaction centers from Rhodopseudomonas viridis

Crystallized reaction centers from Rhodopseudomonas viridis (i) are photochemically active with electron transfer from the special pair to the quinones, (ii) show dichroism giving valuable information on the orientation of the different chromophores and (iii) allow chemical treatment in the crystalline phase

Correlation of structural and spectroscopic properties of a photosynthetic reaction center

Polarized spectra of absorption and light-induced absorbance changes are presented for the crystallized reaction centers of Rhodopseudomonas viridis. We find that a model based on extended dipole interaction between all six pigments is capable of interpreting detailed features such as the contributions from the individual pigments to the various absorption peaks. Even though the pigments are arranged in approximate C2 symmetry, the optical spectra together with the calculations reflect deviations from this symmetry, which may be important in understanding the electron pathway

Structural and Functional Hierarchy in Photosynthetic Energy Conversion—from Molecules to Nanostructures

Basic principles of structural and functional requirements of photosynthetic energy conversion in hierarchically organized machineries are reviewed. Blueprints of photosynthesis, the energetic basis of virtually all life on Earth, can serve the basis for constructing artificial light energy-converting molecular devices. In photosynthetic organisms, the conversion of light energy into chemical energy takes places in highly organized fine-tunable systems with structural and functional hierarchy. The incident photons are absorbed by light-harvesting complexes, which funnel the excitation energy into reaction centre (RC) protein complexes containing redox-active chlorophyll molecules; the primary charge separations in the RCs are followed by vectorial transport of charges (electrons and protons) in the photosynthetic membrane. RCs possess properties that make their use in solar energy-converting and integrated optoelectronic systems feasible. Therefore, there is a large interest in many laboratories and in the industry toward their use in molecular devices. RCs have been bound to different carrier matrices, with their photophysical and photochemical activities largely retained in the nano-systems and with electronic connection to conducting surfaces. We show examples of RCs bound to carbon-based materials (functionalized and non-functionalized single- and multiwalled carbon nanotubes), transitional metal oxides (ITO) and conducting polymers and porous silicon and characterize their photochemical activities. Recently, we adapted several physical and chemical methods for binding RCs to different nanomaterials. It is generally found that the P(+)(Q(A)Q(B))(−) charge pair, which is formed after single saturating light excitation is stabilized after the attachment of the RCs to the nanostructures, which is followed by slow reorganization of the protein structure. Measuring the electric conductivity in a direct contact mode or in electrochemical cell indicates that there is an electronic interaction between the protein and the inorganic carrier matrices. This can be a basis of sensing element of bio-hybrid device for biosensor and/or optoelectronic applications