VEB-1 Extended-Spectrum β-lactamase–producing Acinetobacter baumannii, France1

VEB-1 extended-spectrum β-lactamase–producing Acinetobacter baumannii was responsible for an outbreak in hospitals in France. A national alert was triggered in September 2003 when 4 hospitals reported clusters of A. baumannii infection with similar susceptibility profiles. Case definitions and laboratory guidelines were disseminated, and prospective surveillance was implemented; strains were sent to a single laboratory for characterization and typing. From April 2003 through June 2004, 53 hospitals reported 290 cases of A. baumannii infection or colonization; 275 isolates were blaVEB-1-positive and clonally related. Cases were first reported in 5 districts of northern France, then in 10 other districts in 4 regions. Within a region, interhospital spread was associated with patient transfer. In northern France, investigation and control measures led to a reduction of reported cases after January 2004. The national alert enabled early control of new clusters, demonstrating the usefulness of early warning about antimicrobial drug resistance

Deciphering the Theobroma cacao self-incompatibility system: from genomics to diagnostic markers for self-compatibility

Cocoa self-compatibility is an important yield factor and has been described as being controlled by a late gameto-sporophytic system expressed only at the level of the embryo sac. It results in gametic non-fusion and involves several loci. In this work, we identified two loci, located on chromosomes 1 and 4 (CH1 and CH4), involved in cocoa self-incompatibility by two different processes. Both loci are responsible for gametic selection, but only one (the CH4 locus) is involved in the main fruit drop. The CH1 locus acts prior to the gamete fusion step and independently of the CH4 locus. Using fine-mapping and genome-wide association studies, we focused analyses on restricted regions and identified candidate genes. Some of them showed a differential expression between incompatible and compatible reactions. Immunolocalization experiments provided evidence of CH1 candidate genes expressed in ovule and style tissues. Highly polymorphic simple sequence repeat (SSR) diagnostic markers were designed in the CH4 region that had been identified by fine-mapping. They are characterized by a strong linkage disequilibrium with incompatibility alleles, thus allowing the development of efficient diagnostic markers predicting self-compatibility and fruit setting according to the presence of specific alleles or genotypes. SSR alleles specific to self-compatible Amelonado and Criollo varieties were also identified, thus allowing screening for self-compatible plants in cocoa populations. (Résumé d'auteur

Cellular localisation of VvRops and VvRabA5e, small GTPases developmentally regulated in grape berries

Equipe AFEF ‘Architecture et Fonctionnement des Espèces fruitières’. Correspondance auteur: E-mail: [email protected] audienceVvRops, in particular VvRop9, and VvRabA5e are small GTPases which are developmentally regulated in grape berries. In an attempt to help elucidate the role of these proteins during fruit development and ripening, we investigated their localisation in the fruit by immunocytofluorescence. These proteins were observed at a perinuclear location, at cell periphery and around vesicles. In particular VvRops were found to be located in the nucleus and likely on the plasma membrane. VvRop9 and VvRabA5e cDNAs were introduced separately into S. cerevisiae mutants with RHO1 and YPT31/YPT32 defective genes respectively. Neither cDNAs could complement these temperature-sensitive mutants, suggesting that the functions of the VvRop9 and VvRabA5e genes in grapevine likely differ from the functions of RHO1 and YPT31/YPT32 genes in yeas

Concomitant cases of disseminated Geotrichum clavatum infections in patients with acute myeloid leukemia

International audienc

Encyclopédie des historiographies : Afriques, Amériques, Asies

Quels rapports les sociétés humaines entretiennent-elles avec leur passé et quels récits font-elles du temps révolu ? Pour ce premier volume de l’Encyclopédie des historiographies. Afriques, Amériques, Asies, 157 spécialistes représentant 88 institutions académiques en France et dans le monde explorent l’univers des productions humaines qui constituent des sources pour l’historien et déchiffrent les nombreuses modalités (« scientifiques », littéraires, artistiques, monumentales…) de l’écriture du passé. Évoquant tour à tour l’Afrique, l’Amérique latine, l’Asie, l’Océanie, les 216 notices de l’ouvrage présentent des matériaux historiques de toute nature, issus de toutes les époques, souvent méconnus, ainsi que l’histoire de leurs usages. L’entreprise collective qu’est l’Encyclopédie se veut novatrice : il s’agit de susciter une réflexion historiographique résolument non-occidentalo-centrée qui complète utilement les démarches épistémologiques traditionnelles. Nouvel outil de connaissance historique forgé à l’heure de la mondialisation, l’Encyclopédie des historiographies est aussi une véritable invitation au voyage.What are the different types of relations that non-Western societies upkeep with their past and how are narratives about the past produced by them? In this first volume of the Encyclopaedia of Historiography: Africa, America, Asia, 157 specialists from 88 international academic institutions explore the wealth of evidence that constitutes source material for historians. They also examine the immensely diverse modes or genres of narrated history: “scientific”, literary, artistic, architectural, etc. 216 entries dealing with Africa, Latin America, Oceania, and Asia, cover a large variety of sources, including many which are unfamiliar to the Western or non-Western reader, along with the history of how they have been exploited. By bringing together for the first time such an abundance of material the reader is offered the possibility of exploring continents and building meaningful connections across space and time. In addition to being a new tool for historical enquiry in an era of globalization, this encyclopaedia is also an invitation to travel the world