11 research outputs found

    Computed tomography of the coelomic cavity in healthy veiled chameleons (Chamaeleo calyptratus) and panther chameleons (Furcifer pardalis)

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    Veiled chameleon (Chamaeleo calyptratus) and panther chameleon (Furcifer pardalis) are two of the most popular pet chameleons, and consequently, these species are frequently evaluated in veterinary practices. According to our review of the literature, normal computed tomography (CT) anatomy of these lizards has not been previously described. The purposes of this prospective study were to describe the normal CT anatomy of the coelomic organs in healthy patients and to provide normal reference values in these species. Seventeen clinically healthy veiled chameleons and 15 clinically healthy panther chameleons were included. All CT studies were performed with the chameleons under light anesthesia and positioned in sternal recumbency. Studies were performed with a 16-slice helical CT scanner with a slice thickness of 0.625 mm. The authors recorded qualitative and quantitative CT characteristics of the coelomic structures. Macroscopic cross-sectional anatomy was performed for comparison of the CT findings. Heart, lungs, liver, including caudal vena cava, hepatic vessels, gallbladder, esophagus, stomach, intestines, gonads, fat bodies, kidneys, and urinary bladder could be visualized with CT. The spleen, pancreas, and adrenal glands could not be identified. This study provides a guide to the normal cross-sectional and computed tomographic anatomy of the coelomic cavity in veiled and panther chameleons. Our results could be used as a reference for future research studies or comparison of clinically ill patients

    Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year

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    Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4-CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.info:eu-repo/semantics/publishedVersio

    Immunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one year

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    Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4-CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.info:eu-repo/semantics/publishedVersio

    CITES : aplicació en fauna salvatge i clínica d'animals exótics

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    CITES: Convention on International Trade in Endangered Species of Wild Fauna and Flora = Conveni sobre el Comerç Internacional d'Espècies Amenaçades de Fauna i Flora Silvestre.Treball presentat a l'assignatura de Deontologia i Veterinària Legal (21223

    CITES : aplicació en fauna salvatge i clínica d'animals exótics /

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    CITES: Convention on International Trade in Endangered Species of Wild Fauna and Flora = Conveni sobre el Comerç Internacional d'Espècies Amenaçades de Fauna i Flora Silvestre.Treball presentat a l'assignatura de Deontologia i Veterinària Legal (21223

    Intracranial Granular Cell Tumours in Three Dogs: Atypical Magnetic Resonance Imaging Features and Immunohistochemical Study

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    Intracranial granular cell tumours (GCT) are uncommon neoplasms of uncertain cellular origin that are rarely reported in dogs. This case series describes three aged dogs that presented with neurological signs in which magnetic resonance (MR) imaging revealed plaquelike extra-axial lesions that were hypointense on T2-weighted (T2w) images. The surgical biopsy of the lesions and necropsies were followed by histochemical characterisation with periodic acid–Schiff (PAS) staining and immunohistochemistry with ubiquitin, S-100, and SOX-10 to elucidate the cellular origin. The immunohistochemical study indicated that these intracranial GCTs were not of Schwann cell origin. In conclusion, GCTs should be considered a differential diagnosis of intracranial, extra-axial hypointense brain lesions on T2w MR images

    Diagnosis and treatment of congenital hydrocephalus in a red fox (Vulpes vulpes) with seizures

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    Acord transformatiu CRUE-CSICA 2-year-old male red fox (Vulpes vulpes) was presented for generalised tonic-clonic seizures, blindness, compulsive circling and behavioural changes that occurred since the animal was 2 months old. Neurological examination indicated a diffuse forebrain lesion. On magnetic resonance imaging of the brain, a severe dilation of both lateral ventricles was identified. Congenital hydrocephalus was suspected and the fox was treated with methylprednisolone, omeprazole and phenobarbital. Sixteen months later, the neurological signs had improved: the frequency of seizures had decreased to one seizure every 3 months and compulsive circling was less pronounced

    Intracranial Granular Cell Tumours in Three Dogs : Atypical Magnetic Resonance Imaging Features and Immunohistochemical Study

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    Intracranial granular cell tumours are rare tumours of unknown cellular origin. In this case series. we describe three dogs with neurological signs that had magnetic resonance imaging of the brain, and were diagnosed with granular cell tumours through histopathological and histochemical studies. Magnetic resonance imaging characteristics were atypical, showing hypointense lesions on T2-weighted images in all cases. Immunohistochemical studies helped in characterising the tumours and excluded a Schwann cell origin. The findings of this case series indicate that granular cell tumours can be hypointense on T2-weighted magnetic resonance images, and that the cell of origin remains to be determined. Intracranial granular cell tumours (GCT) are uncommon neoplasms of uncertain cellular origin that are rarely reported in dogs. This case series describes three aged dogs that presented with neurological signs in which magnetic resonance (MR) imaging revealed plaquelike extra-axial lesions that were hypointense on T2-weighted (T2w) images. The surgical biopsy of the lesions and necropsies were followed by histochemical characterisation with periodic acid-Schiff (PAS) staining and immunohistochemistry with ubiquitin, S-100, and SOX-10 to elucidate the cellular origin. The immunohistochemical study indicated that these intracranial GCTs were not of Schwann cell origin. In conclusion, GCTs should be considered a differential diagnosis of intracranial, extra-axial hypointense brain lesions on T2w MR images

    Immunogenicity and Protection against Mycobacterium caprae Challenge in Goats Vaccinated with BCG and Revaccinated after One Year

    No full text
    Vaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4−CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae

    Glossari il·lustrat de morfologia botànica bàsica. Racons verds: aportació al coneixement morfològic dels espermatòfits

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    Els resultats s’emmarquen en el projecte d’innovació docent «Innovació en l’ambientalització curricular de la Botànica en el grau de Farmàcia: Jardins per a la Salut» (codi 2018PID-UB/034) del Grup d’Innovació Docent de Botànica Aplicada a les Ciències Farmacèutiques (GIBAF).Facultat de Farmàcia, Universitat de Barcelona. Ensenyament: Grau de Farmàcia. Assignatura: Botànica farmacèutica. Curs: 2017-2018. Coordinadors: Cèsar Blanché, Carles Benedí, Maria Bosc i Joan SimonProjecte: 2018PID-UB/034Es presenta a continuació un glossari il·lustrat realitzat per 35 estudiants de l’assignatura Botànica Farmacèutica del grau de Farmàcia. Els resultats s’emmarquen en el projecte d’innovació docent «Innovació en l’ambientalització curricular de la Botànica en el grau de Farmàcia: Jardins per a la Salut» (codi 2018PID-UB/034) del Grup d’Innovació Docent de Botànica Aplicada a les Ciències Farmacèutiques (GIBAF). El glossari s’ha il·lustrat a partir de fotografies dels estudiants sobre detalls morfològics de les espècies del jardí de la Facultat de Farmàcia que s’ha inclòs en els «Racons Verds de la UB». El resultat ha estat la descripció i il·lustració de 80 caràcters d’organografia vegetativa i reproductora i constitueixen un nou recurs docent en obert realitzat pels propis estudiants. El treball dut a terme s’ha realitzat de forma voluntària, tutoritzada i restringida en tres grups de teoria (M2, M3 i T3) de l'assignatura troncal de Botànica Fermacèutica del grau de Farmàcia. Els resultats hanrepercutit fins a 0,5 punts sobre la nota final un cop s’ha superat l’assignatura.Grup d'Innovació Docent en Botànica Aplicada a les Ciències Farmacèutiques (GIBAF
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