39 research outputs found
Effect of Different Functional Food Supplements on the Gut Microbiota of Prediabetic Indonesian Individuals during Weight Loss
The gut microbiota has been shown in recent years to be involved in the development
and severity of type 2 diabetes (T2D). The aim of the present study was to test the effect of a 2-
week functional food intervention on the gut microbiota composition in prediabetic individuals. A
randomized double-blind, cross-over trial was conducted on prediabetic subjects. Fifteen volunteers
were provided products made of: (i) 50% taro flour + 50% wheat flour; (ii) these products and the
probiotic L. plantarum IS-10506; or (iii) these products with beetroot adsorbed for a period of 2 weeks
with 2 weeks wash-out in between. Stool and blood samples were taken at each baseline and after
each of the interventions. The gut microbiota composition was evaluated by sequencing the V3–V4
region of the 16S rRNA gene and anthropometric measures were recorded. The total weight loss
over the entire period ranged from 0.5 to 11 kg. The next-generation sequencing showed a highly
personalized microbiota composition. In the principal coordinate analyses, the samples of each
individual clustered closer together than the samples of each treatment. For six individuals, the
samples clustered closely together, indicating a stable microbiota. For nine individuals, the microbiota
was less resilient and, depending on the intervention, the beta-diversity transiently differed greatly
only to return to the composition close to the baseline during the wash-out. The statistical analyses
showed that 202 of the total 304 taxa were significantly different between the participants. Only
Butyricimonas could be correlated with taro ingestion. The results of the study show that the highly
variable interindividual variation observed in the gut microbiota of the participants clouded any gut
microbiota modulation that might be present due to the functional food interventions.
Keywords: taro; probiotic; L. plantarum IS-10506; beetroot; gut microbiota; prediabete
HRMAS 1H NMR as a tool for the study of supramolecular gels
HRMAS 1H NMR is reported for the first time as a useful technique to gain insight into the dynamic properties of aggregates present in supramolecular gels. The study of several low molecular weight gelators with this technique in toluene and acetonitrile is described
Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats.
This study aims at assessing the conversion of exogenous D-[1-13C]fructose, D-[2-13C]fructose or D-[6-13C]-fructose (10 mM) to 13C-enriched and either hydrogenated or deuterated D-glucose, L-lactate and L-alanine released by rat liver cells prepared from Goto-Kakizaki rats and incubated for 120 min in the presence of unlabelled D-glucose (also 10 mM) and D2O. The results of this study are relevant to the relative contribution of fructokinase and hexokinase isoenzyme to the phosphorylation of D-fructose, the capacity of D-glucose to confer to glucokinase positive cooperativity towards D-fructose, the circulation of D-fructose 6-phosphate in the pentose phosphate pathway, the regulation of the cytosolic NADD/NADH ratio, the respective fate of D-fructose-derived D-glyceraldehyde and dihydroxyacetone phosphate, the deuteration of fructose-derived glycolytic intermediates at the phosphoglucoisomerase, phosphomannoisomerase, enolase, pyruvate kinase and glutamate-alanine transaminase levels, and the unequal generation of L-[1-13C]lactate by cells exposed to D-[1-13C]fructose or D-[6-13C]fructose versus D-[2-13C]-fructose.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe
Metabolism of D-[1-(13)C]fructose, D-[2-(13)C]fructose, and D-[6-(13)C]fructose in rat hepatocytes incubated in the presence of H(2)O or D(2)O.
Isolated hepatocytes from fed rats were exposed for 120 min to D-[1-(13)C]fructose, D-[2-(13)C]fructose, or D-[6-(13)C]fructose in the presence of H(2)O or D(2)O. The identification and quantification of (13)C-enriched metabolites (D-glucose, L-lactate) in the incubation medium and the measurement of their deuterated isotopomers indicated that the ketohexose was phosphorylated predominantly at the intervention of fructokinase and that the majority of the D-glyceraldehyde molecules generated from d-fructose 1-phosphate were further metabolized, e.g. after phosphorylation to D-glyceraldehyde 3-phosphate. It is proposed that the present procedure may help to further characterize the regulation of D-fructose metabolism in both hepatocytes and other cell types.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe
Delayed interconversion of D-glucose anomers in D2O.
SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Effects of D-glucose upon D-fructose metabolism in rat hepatocytes: A 13C NMR study.
Isolated hepatocytes from fed rats were exposed for 120 min to D-glucose (10 mM) and either D-[1-13C]fructose, D-[2-13C]fructose or D-[6-13C]fructose (also 10 mM) in the presence of D2O. The identification and quantification of 13C-enriched D-fructose and its metabolites (D-glucose, L-lactate, L-alanine) in the incubation medium and the measurement of their deuterated isotopomers indicated, by comparison with a prior study conducted in the absence of exogenous D-glucose, that the major effects of the aldohexose were to increase the recovery of 13C-enriched D-fructose, decrease the production of 13C-enriched D-glucose, restrict the deuteration of the 13C-enriched isotopomers of D-glucose to those generated by cells exposed to D-[2-13C]fructose, and to accentuate the lesser deuteration of the C, (as compared to C5) of 13C-enriched D-glucose derived from D-[2-13C]fructose. The ratio between C2-deuterated and C2-hydrogenated L-lactate, as well as the relative amounts of the CH3-, CH2D-, CHD, and CD3- isotopomers of 13C-enriched L-lactate were not significantly different, however, in the absence or presence of exogenous D-glucose. These findings indicate that exogenous D-glucose suppressed the deuteration of the C1 of D-[I-13C]glucose generated by hepatocytes exposed to D-[1-13C]fructose or D-[6-13C]fructose, as otherwise attributable, in part at least, to gluconeogenesis from fructose-derived [3-13C]pyruvate, and apparently favoured the phosphorylation of D-fructose by hexokinase isoenzymes, probably through stimulation of D-fructose phosphorylation by glucokinase.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe
Asymmetrical labeling of D-glucose generated from [3(-13)C]pyruvate in rat hepatocytes.
The generation of 13C-labeled D-glucose isotopomers by rat hepatocytes incubated for 30 or 120 min in the presence of 10 mM [3-(13)C]pyruvate was assessed by 13C NMR. The amount of C1-labeled D-glucose exceeded that of C2-labeled hexose, which was itself higher than that of C3-labeled D-glucose. A comparable hierarchy was observed in the C6-C5-C4 moiety of the hexose. The latter moiety of D-glucose was more efficiently labeled, however, than the C3-C2-C1 moiety. This finding is similar to that both previously reported and again observed in the present study when hepatocytes were exposed to [2(-13)C]pyruvate. These converging observations thus support the concept of enzyme-to-enzyme channeling of D-glyceraldehyde 3-phosphate between glyceraldehyde-3-phosphate dehydrogenase and phospho-fructoaldolase.In VitroJournal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe
D-glucose generation from [2-13C]pyruvate in rat hepatocytes: Implications in terms of enzyme-to-enzyme channelling
SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Enzyme-to-enzyme channelling of Krebs cycle metabolic intermediates in Caco-2 cells exposed to [2-13C]propionate
SCOPUS: ar.jinfo:eu-repo/semantics/publishe