Scaling the spectral beam combining channels in a multiplexed volume Bragg grating

In order to generate high power laser radiation it is often necessary to combine multiple lasers into a single beam. The recent advances in high power spectral beam combining using multiplexed volume Bragg gratings recorded in photo-thermo-refractive glass are presented. The focus is on using multiple gratings recorded within the same volume to lower the complexity of the combining system. Combining of 420 W with 96% efficiency using a monolithic, multiplexed double grating recorded in PTR glass is demonstrated. A multiplexed quadruple grating that maintains high efficiency and good beam quality is demonstrated to pave a way for further scaling of combining channels

A handbook of Nebraska grasses : with illustrated keys for their identification, together with a general account of their structure and economic importance

The true grasses belong to the one family Poaceae, often called Gramineae, and constitute a very well defined natural group of plants. This family comprises upward of 3,000 species distributed among almost 300 genera. From the economic point of view the family is of supreme importance thru the fact that here belong the cereals, which supply a large part of the food of man, and the forage grasses which are so important as feed for our domesticated animals. The genera and species are for the most part separated by artificial characters—a fact which renders their study and identification extremely difficult even for the expert. The experience of the authors has convinced them that much of this difficulty is to be credited to the keys found in our common manuals. Theoretically a key is intended to assist one in properly identifying a plant, but actually many of the keys, upon close inspection. are found to contain so many vague and contradictory statements that they can be depended upon only by the expert who, on account of his working knowledge, makes slight use of the keys. It occurred to the senior author some years ago that an illustrated key might solve many of these difficulties. An accurate illustration conveys a more definite idea of any particular structure and leaves less room for doubt than any number of words. In fact, the serious and annoying inaccuracies of most keys to the grasses became more and more apparent as the work of preparing the illustrations progressed. The authors are fully convinced that illustrated keys of the type here employed will prove useful in many other groups of plants. The nomenclature here employed is largely that of the seventh edition of Gray\u27s Manual—tho our purpose has not been to insist upon any particular name but to provide means whereby the student can with some certainty attach to any given plant some one name which has been properly authorized. The present key includes most of the species known to occur in Nebraska, tho a few of minor importance and very restricted distribution have been omitted. There are provided also some data on the economic value of certain of the most important species. The illustrations for the grass keys have been drawn expressly for this publication. They are derived in part from actual authentic specimens and in part redrawn in modified form from various manuals and monographs. T he authors have made free use of the manuals, monographs. etc., included in the Bibliography and take this opportunity to commend these publications to the attention of all who wish to learn more of our common grasses. It is hoped that the keys here presented will facilitate the study of the grass flora of Nebraska by farmers and students

Measurement of the Tau Lepton Polarisation at LEP2

A first measurement of the average polarisation P_tau of tau leptons produced in e+e- annihilation at energies significantly above the Z resonance is presented. The polarisation is determined from the kinematic spectra of tau hadronic decays. The measured value P_tau = -0.164 +/- 0.125 is consistent with the Standard Model prediction for the mean LEP energy of 197 GeV.A first measurement of the average polarisation Pτ of tau leptons produced in e + e − annihilation at energies significantly above the Z resonance is presented. The polarisation is determined from the kinematic spectra of tau hadronic decays. The measured value Pτ=−0.164±0.125 is consistent with the Standard Model prediction for the mean LEP energy of 197 GeV.A first measurement of the average polarisation P_tau of tau leptons produced in e+e- annihilation at energies significantly above the Z resonance is presented. The polarisation is determined from the kinematic spectra of tau hadronic decays. The measured value P_tau = -0.164 +/- 0.125 is consistent with the Standard Model prediction for the mean LEP energy of 197 GeV

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Spectral Stabilization Of High Efficiency Diodes And Diode Bars By External Bragg Resonator

Adjustable spectral and angular selectivity combined with low optical losses and high laser damage thresholds of volume Bragg gratings, which are recorded in photo-thermo-refractive (PTR) glass, enabled the use of those elements as high efficient selective feedback elements for spectral narrowing and stabilization. High efficiency diodes and diode bars developed by AlfaLight and nLight companies for DARPA/SHEDS program were studied in volume Bragg lasers with PTR holographic gratings used as output coupling elements. Reflection coefficients from the front facets of the bars were varied from 0.5 to 5%. Reflection coefficients of PTR Bragg gratings were varied from 3 to 50%. It was found that for any applicable pumping level the optimal parameters of the grating could be found which provide spectral narrowing and stabilization within 0.5nm for temperature variations within ±10 K. Total power of the locked bar exceeded 97% of that for the unlocked bar

Semiconductor 1.7 W Volume Bragg Laser With Divergence Close To A Diffraction Limit

Novel volume Bragg laser (VOBLA) with two photo-thermo-refractive volume Bragg gratings feedback is studied on the basis of 150-um-stripe LD. High-efficient, near diffraction-limited operation at power of 1.7W and spectral width of 250pm are demonstrated. © 2004 Optical Society of America

Beam Combining Of Lasers With High Spectral Density Using Volume Bragg Gratings

Incoherent combining of multiple laser beams with offset wavelengths into a single near-diffraction-limited beam is an effective solution to increasing energy brightness and scaling output power of high-power lasers. Volume Bragg gratings (VBGs) recorded in photo-thermo-refractive (PTR) glass allow spectral beam combining with a remarkably high spectral density of channels. Spectral beam combining (SBC) of five channels within 1-2 nm bandwidth around 1064 and 1550 nm into a single near-diffraction-limited beam with absolute efficiency 92-94% is demonstrated. Scaling of this technique to multi-kW power level is discussed. © 2009 Elsevier B.V

Spectral Beam Combining Of Fiber Lasers By Volume Bragg Gratings

Output power of 770 W from a system combining five fiber lasers with 91.7% efficiency is demonstrated with spectral separation between channels of 0.5 nm and no distortions in diffracted beams. © 2008 OSA