Safety assessment of the functional feed additive phenylcapsaicin in a commercial broiler diet

Introduction: Intestinal colonisation of Salmonella is a major concern in the poultry industry, and a low dose of the high-purity synthetic capsaicin analogue phenylcapsaicin (PheCap) has the potential to be a phytobiotic alternative to antibiotics in reducing floor Salmonella in commercial broiler chicken houses. In this study we present the first safety assessment of PheCap at doses relevant for the poultry industry. Methods: In a completely randomized block design, Ross 308 male broilers were offered feed containing 0, 10, 15, or 150 mg PheCap/kg. Growth rates, mortality, haematology, clinical chemistry, foot pad lesions, litter quality and gross pathological examination of organs and tissues were evaluated for signs of toxicity over a two-phase, 35-day growth period. Results: No differences in feed intake and broiler growth were found, with broilers in the control group having the highest mortality. There was a statistically significant increase in the European Production Efficiency Factor (EPEF) for the 10 (p = 0.02) and 15 mg PheCap/kg feed (p = 0.003) treatment doses. No dose dependent adverse effects were found for any of the treatment doses. The No Observed Adverse Effect Level (NOAEL) of PheCap is probably higher than that of the highest weekly averaged daily intake of 36.3 mg/kg BW/day observed in the present study. Conclusions: The inclusion of PheCap in broiler feed at doses relevant for the commercial poultry industry is assumed not have any negative effects on broiler health.publishedVersio

Introducing insect- or plant-based dinner meals to families in Denmark: study protocol for a randomized intervention trial

Background: Plant- and insect-based foods are promising alternative protein sources. Previous studies have shown that introducing plant-based foods to the diet can reduce meat intake, but no such intervention has explored the effects of insect-based foods. Methods: This study aims to integrate alternative proteins to main meals of 80 Danish families through a 6-week two-arm randomized intervention trial to investigate acceptance, consumption, and potential for meat replacement. The primary outcome is the replacement of dietary meat protein with plant- or insect-based protein from the intervention foods assessed through change in daily meat protein intake, proportion of meat protein to total protein intake, and counts of dinner meals with meat and intervention products. Conclusion: The results of this study will contribute to research in alternative proteins and explore the effects of long-term exposure of meat replacement. Trial registration: ClinicalTrials.gov: NCT05156853.info:eu-repo/semantics/publishedVersio

Do foot pad scores measure Turkey welfare

The main aim of the project was to assess the painfulness of different levels of foot pad dermatitis (FPD) in turkeys. Three different analgesics (butorphanol, carprofen and meloxicam) were used to assess their effect on behaviour. Video recordings were taken when the birds were treated with either saline or one of the analgesics in a crossover experiment, with the aim of investigating whether or not FPD is a painful condition. The results did not show significant pain in affected turkeys. Carprofen may have an effect on behavior, whereas butorphanol and meloxicam did not

Bioconversion of Digestate, Pig Manure and Vegetal Residue-Based Waste Operated by Black Soldier Fly Larvae, Hermetia illucens L. (Diptera: Stratiomyidae)

Insects can play an important role to upgrade waste streams into high-grade proteins and fats as food and feed ingredients or non-food products. The aim of this research was to assess the feasibility to use waste streams with a low value for direct application as animal feed as substrates to grow BSF larvae in terms of larval growth rate, waste reduction index, and efficiency of conversion of ingested feed. The growth of black soldier fly (BSF), Hermetia illucens larvae and conversion of biowaste was assessed in triplicate in biowaste substrates: chicken feed (CF; reference diet), pig manure solid (PMS), Betafert® solid (BTFS), swill (SW), olive pulp (OP), pig manure liquid mixed with chicken feed (PMLCF), and silage grass (SG). Per kilogram fresh substrate 2500 starter (8-days-old, second instar) larvae were incubated in 21 plastic containers (75 × 47 × 15 cm). The BSF larvae were fed according to a batch feeding system. Highest growth rate was found in larvae reared on SW (13.4 mg/d). Larval growth rate was even higher than in larvae reared on the reference substrate CF (7.2 mg/d). Growth rate in larvae reared on PMLCF (7.3 mg/d) did not differ from CF, whereas growth rate of larvae reared on PMS (3.2 mg/d) was lower than on CF. Growth rate of larvae reared on BTFS, OP and SG was very low (0.6, 0.2 and 0.7 mg/d, respectively). Waste Reduction Index (WRI) was highest on SW (11.3), followed by PMLCF (9.3), and both were higher than WRI on CF (8.5). Waste Reduction Index further decreased in descending order from PMS, SG, BTFS to OP (7.6, 4.0, 2.9 and 1.7, respectively). The Efficiency of Conversion of Ingested substrate (ECI) was highest on SW (0.31), followed in descending order by PMLCF, CF and PMS (0.25, 0.21 and 0.18, respectively). The substrates OP, BTFS and SG (0.16, 0.15 and 0.14, respectively) resulted in a lower ECI than other substrates. Highest CO2 and lowest NH3 concentrations were found above substrates with the highest larval growth performances. This study showed that BSF larvae can be reared on different biowaste substrates; the growth rate of the larvae was extremely high on SW. The effects of chemical composition and physical properties of the substrates on larval growth and gas emissions should be further considered

Do foot pad scores measure Turkey welfare

The main aim of the project was to assess the painfulness of different levels of foot pad dermatitis (FPD) in turkeys. Three different analgesics (butorphanol, carprofen and meloxicam) were used to assess their effect on behaviour. Video recordings were taken when the birds were treated with either saline or one of the analgesics in a crossover experiment, with the aim of investigating whether or not FPD is a painful condition. The results did not show significant pain in affected turkeys. Carprofen may have an effect on behavior, whereas butorphanol and meloxicam did not

Growth efficiency, intestinal biology, and nutrient utilization and requirements of black soldier fly (Hermetia illucens) larvae compared to monogastric livestock species : a review

In recent years, interest in the larvae of black soldier fly (BSF) (Hermetia illucens) as a sustainable protein resource for livestock feed has increased considerably. However, knowledge on the nutritional and physiological aspects of this insect, especially compared to other conventional farmed animals is scarce. This review presents a critical comparison of data on the growth potential and efficiency of the BSF larvae (BSFL) compared to conventional monogastric livestock species. Advantages of BSFL over other monogastric livestock species includes their high growth rate and their ability to convert low-grade organic waste into high-quality protein and fat-rich biomass suitable for use in animal feed. Calculations using literature data suggest that BSFL are more efficient than broilers, pigs and fish in terms of conversion of substrate protein into body mass, but less efficient than broilers and fish in utilization of substrate gross energy to gain body mass. BSFL growth efficiency varies greatly depending on the nutrient quality of their dietary substrates. This might be associated with the function of their gastrointestinal tract, including the activity of digestive enzymes, the substrate particle characteristics, and their intestinal microbial community. The conceived advantage of BSFL having an environmental footprint better than conventional livestock is only true if BSFL is produced on low-grade organic waste and its protein would directly be used for human consumption. Therefore, their potential role as a new species to better close nutrient cycles in agro-ecological systems needs to be reconsidered, and we conclude that BSFL is a complementary livestock species efficiently utilizing organic waste that cannot be utilized by other livestock. In addition, we provide comparative insight into morpho-functional aspects of the gut, characterization of digestive enzymes, gut microbiota and fiber digestion. Finally, current knowledge on the nutritional utilization and requirements of BSFL in terms of macro- and micro-nutrients is reviewed and found to be rather limited. In addition, the research methods to determine nutritional requirements of conventional livestock are not applicable for BSFL. Thus, there is a great need for research on the nutrient requirements of BSFL

Supplementary data to publication "Growth efficiency, intestinal biology, and nutrient utilization and requirements of black soldier fly (Hermetia illucens) larvae compared to monogastric livestock species: a review"

The datasets provided in the excel file were used to generate Table 2 and Fig. 2 in the publication entitled "Growth efficiency, intestinal biology, and nutrient utilization and requirements of black soldier fly (Hermetia illucens) larvae compared to monogastric livestock species: a review" by Seyedalmoosavi et al., 2022 published in Journal of Animal Science and Biotechnology. https://doi.org/10.1186/s40104-022-00682-