Protocolos hormonais para produção in vitro de embriões de zebuínos e taurinos

The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound‑guided follicular aspirations (n=237) were performed without pre‑treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick‑up and in vitro production of embryos in both cattle subspecies evaluated.O objetivo deste trabalho foi avaliar os efeitos de protocolos hormonais de sincronização, associados ou não com o estímulo ao desenvolvimento folicular, na recuperação de oócitos e na produção in vitro de embriões de Bos indicus e B. taurus, em diferentes estações. As aspirações foliculares guiadas por ultrassom (n=237) foram realizadas sem pré‑tratamento (G1, grupo controle) e após sincronização da onda folicular (G2), ou após sincronização da onda folicular e estímulo do desenvolvimento folicular (G3). Bos indicus produziu mais oócitos e embriões que B. taurus (18,7±0,9 vs. 11,9±0,6 oócitos e 4,8±0,3 vs. 2,1±0,2 embriões). Em média, as produções de oócitos e embriões foram maiores em G3 do que em G2, e ambas foram maiores que em G1, o que levou a uma maior conversão de oócitos em embriões nesses tratamentos. A estação quente ou fria não afetou os resultados de B. indicus, enquanto, em B. taurus, tanto a recuperação de oócitos quanto a produção de embriões foram maiores na estação fria. A sincronização da onda folicular melhora a aspiração folicular e a produção in vitro de embriões, em ambas as subespécies de bovinos avaliadas

Cloxacillin nanostructured formulation for the treatment of bovine keratoconjunctivitis.

Infectious bovine keratoconjunctivitis (IBK) is a widespread, contagious ocular disease that affects cattle, especially dairy breeds. The disease is caused by Gram-negative bacteria mainly Moraxella bovis, and its treatment consists of parenteral or topic antibiotic therapy. The topic treatment approach is used more commonly in lactating cows, to avoid milk disposal. However, treatment failures are common, because the antibiotic is removed during lacrimation. This study aimed to evaluate the susceptibility of commercial cloxacillin and evaluate the efficacy of nanostructured cloxacillin in clinical cases of IBK by Moraxella. The minimum inhibitory concentration (MIC) of nanoparticle cloxacillin nanocoated, the nanoparticle without the antibiotic and the commercial cloxacillin were determined in vitro with field samples of Moraxella ovis (5) and Moraxella bovis (5). The efficiency of nanoparticles was tested in three cows naturally infected that were treated with 1.0 mL (with 0.32 mg of nanostructured cloxacillin) for the ocular route. Moraxella bovis was isolated and identified by biochemical and molecular methods before the treatment. The animals were treated every 12 h for six days. The cure was considered by the absence of clinical symptoms and bacteria after treatment. The mucoadhesive nanoparticle-based formulation promoted clinical cure with a low number of doses of antibiotics, probably due to the maintenance of the MIC in the ocular mucosa for longer due to the mucoadhesive characteristics of the nanoparticle. The results indicate that the use of nanocoated cloxacillin is possible to control infectious bovine keratoconjunctivitis

Hormonal protocols for in vitro production of Zebu and taurine embryos

The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of oocytes to embryos in these treatments. The hot or the cold season did not affect the B. indicus outcomes, whereas, in B. taurus, both oocyte recovery and embryo production were higher in the cold season. Follicular wave synchronization improves ovum pick-up and in vitro production of embryos in both cattle subspecies evaluated

Zusatz von Serin-Protease-Inhibitoren zur Konservierung und Verdünnung des Hengstsamens während Abkühlung und Tiefgefrierung

Submitted by Nuzia Santos ([email protected]) on 2013-10-03T18:56:04Z No. of bitstreams: 1 129.2010.pdf: 143833 bytes, checksum: 173707f7286b82578ee41df3db059daf (MD5)Made available in DSpace on 2013-10-03T18:56:04Z (GMT). No. of bitstreams: 1 129.2010.pdf: 143833 bytes, checksum: 173707f7286b82578ee41df3db059daf (MD5) Previous issue date: 2010Universidade de Uberaba. Instituto de Estudos Avançados em Veterinária José Caetano Borges. Uberaba ,MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunológia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilThe objective of the present work was to examine the consequences of adding serine protease inhibitor and its synthetic analogs to stallion semen during cryopreservation in order to reduce cryocapacitation during cooling and freezing. Semen from six stallions was collected, and samples were cooled to 5ºC following the addition of serine proteases inhibitors a) Benzamidine (5mM, 7.5mM, and 9mM); b) Berenil (1mM, 2.5mM, and 5mM); c) purified inhibitor (4mM) in final concentrations, and other samples were frozen with INRA 82 freezing extender in liquid nitrogen, following the addition of serine proteases inhibitors. Sperm progressive motility was evaluated under phase contrast microscopy, and membrane functional integrity with the hyposmotic swelling test (HOST). Sperm membrane integrity was assessed with propidium iodide stain and acrosome integrity (AI) with FITC-PNA stain, using a flow cytometer, and induction of acrosome reaction with calcium ionophore A23187 (5μM). In the control and treated groups, respectively, the mean values of progressive motility (47.5 % and 48.7 %) and membrane integrity (68.0% and 63.8%) after cooling and of progressive motility (10.8% and 11.8%), membrane integrity (21.4% and 23.4%), HOST+ (24.7% and 25.9%) and acrosome reaction (3.8% and 3.4%) after thawing, did not differ (p>0.05). After the induction of acrosome reaction, no difference (p>0.05) was observed in acrosome reacted sperm between the control (12.7%) and treated groups (16.8%). The tested serine protease inhibitors could not reduce stallion sperm membrane scrambling which occurs during cooling and freezing