Results of a collaborative study on DNA identification of aged bone samples

AimA collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. MethodsEighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. ResultsAlthough the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. ConclusionThe laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized

Transition, Integration and Convergence. The Case of Romania

This volume comprises several studies and papers published in the last decades. They have been selected and ranged so that to provide a minimum of coherence concerning the phases which Romania has crossed in her way to the advanced socio-economic system of European type: transition to the market economy, accession to the EU, the economic convergence in the three fundamental domains: institutions, real economy, and nominal economy. The readers may find in this volume a description of debates, difficulties and solutions adopted for building-up the market economy by a state being in a profound transformation from weak transition institutions towards hard democratic institutions. Because the transition to the market economy and the association of Romania with the EU and then the integration presenting strategic political decisions, I have included in this work two studies devoted to the political forces state and political parties that elaborated and applied these strategic decisions underlining their structure, role and function and their transformation. Integration into the EU of a country like Romania, which emerged from a different system comparing with the West-European one, has proved to be difficult and lasting many years because of the structural transformations. In five chapters I am referring to the essential characteristics of the integration process, such as: market liberalization, competitiveness of the local (national) firms on the national and EU markets, institutional reforms so that the institutions of candidate countries have to become compatible with those of the EU and finally the perspective assessment to find out the real and nominal convergence. Putting into practice the EU competitivity and cohesion principles, Romania has good prospects to close, in a reasonable time, the economic gap and to be admitted into the Euro Zone. Although the real convergence of Romania with the EU requires higher growth rates for the former, a new approach is compulsory to take into consideration the environment quality, the natural resources and the equity between the present and the future generations as natural resource consumers. Just these problems have determined me to include in this volume the last two chapters which, on the one hand, try to prove the necessity of the economy growth harmonization with the environment evolution as well as the saving of the energy resources, and, on the other hand, to point out the main ways to be followed and instruments to be used

Duplications of noncoding elements 5' of SOX9 are associated with brachydactyly-anonychia

status: publishe

Conjugation of haloalkanes by bacterial and mammalian glutathione transferases: Mono- and dihalomethanes

A primary route of metabolism of dihalomethanes occurs via glutathione (GSH) transferase-catalyzed conjugation. Mammalian theta class GSH transferases and a group of bacterial dichloromethane dehalogenases are able to catalyze the hydrolytic dehalogenation of dihalomethanes via GSH conjugation and subsequent formation of HCHO. Dihalomethanes have been shown to induce revertants in Salmonella typhimurium TA 1535 expressing theta class GSH transferases. Two mammalian theta class GSH transferases (rat GST 5-5 and human GST T1) and the bacterial dehalogenase DM11 were compared in the in vitro conjugation of CH3Cl and using in vitro assays (HCHO formation) and the S. typhimurium mutagenesis assay with the dihalomethanes CH2Cl2, CH2Br2, CH2BrCl, CH2ICl, CH2I2, and CH2ClF. GSTs 5-5 and TI had similar characteristics and exhibited first-order rather than Michaelis-Menten kinetics for HCHO formation over the range of dihalomethane concentrations tested. In contrast, the DM11 enzyme displayed typical hyperbolic Michaelis-Menten kinetics for all of the compounds tested. A similar pattern was observed for the conjugation of CH3Cl The reversion tests with S. typhimurium expressing DM11 or GST 5-5 showed a concentration-dependent increase in revertants for most of the dihalomethanes, and DM11 produced revertants at dihalomethane concentrations lower than GST 5-5. Collectively, the results indicate that rates of conversion of dihalomethanes to HCHO are not correlated with mutagenicity and that GSH conjugates are genotoxic. The results are compared with the conjugation and genotoxicity of haloethanes in the preceding paper in this issue [Wheeler, J. B., Stourman, N. V., Armstrong, R. N., and Guengerich, F. P. (2001) Chem. Res. Toxicol. 14, 1107-1117]. The halide order appears most important in the dihalomethane conjugation reactions catalyzed by GST 5-5 and less so in GST T1 and DM11, probably due to changes in the rate-limiting steps

Results of an Aeroelastically Tailored Wing on the FLEXOP Demonstrator Aircraft

The idea of the EU funded FLEXOP project is to raise efficiency of a currently existing wing by derivative solution with higher aspect ratio at no excess structural weight. In order to enable such a resulting highly flexible wing the project goal is to develop methods for active suppression of flutter and passive load alleviation. The developed methods will be tested and validated with a UAV flutter demonstrator. The demonstrator is a 7m wingspan, 65kg MTOW UAV equipped with a jet engine. It features three different wing pairs. The first wing is a stiff design reference case, which is flown to get the baseline measurements for comparison. The second one is a wing designed very flexible specifically for active flutter control. The third wing is aeroelastically tailored for gust load alleviation. The paper describes the results of the aeroelastically tailored wing compared to the baseline reference wing

Ground Testing of the FLEXOP Demonstrator Aircraft

Ground testing campaign conducted on the FLEXOP demonstrator aircraft is presented in this paper. The conducted tests are grouped in structural, flight system and integration tests. Along with the description of the test setup and test execution, the main findings and conclusions are also given. The structural tests comprise the static, ground vibration and the airworthiness test. The static and the ground vibration tests were used for structural characterisation of the manufactured wings and airframe as a whole. Assessment and calibration of the Fibre Brag strain sensing system for wing shape and load reconstruction is also presented within this context. The airworthiness test is used to demonstrate the structural integrity of the manufactured wings under specified limit loads. Within the context of the flight system tests, the main components of the on-board autopilot hardware-software system are briefly introduced including the signal data flow from the RC transmitter to the aircraft controls, the functionality of the baseline autopilot software and the communication with the ground station. All of these components are integrated into the hardware-in-the-loop environment, which is also briefly introduced along with the servo motor identification and the hardware delay measurements. The measured hardware delay was considered in the design of the baseline and flutter controllers. The flutter controllers were tested together with the baseline controller in the software-in-the-loop environment. System integration tests are presented last. In this context the airbrake, the engine, the compatibility of electronic components, the range and the taxi tests are presented.Aerospace Structures & Computational Mechanic