7 research outputs found

    Micro-quantitative method for analysis of sterol levels in honeybees and their pollen loads

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    peer reviewedA Gas Chromatography-Flame Ionization Detector method for characterization and micro-quantification of sterols was developed. The applicability of this method was evaluated for determining sterolic compounds in pollen loads, larvae, and adults of the honeybee. After a multi-step procedure to extract and purify sterols, the compounds were identified on the basis of their retention data and quantified on the basis of peak areas from analyses. Quantifications were feasible with a limited amount of samples, allowing single specimen analyses and estimation of inter-individual variation. The reliability of this microquantitative method for sterol analysis was also investigated
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