PERLINDUNGAN HUKUM DATA PRIBADI PENGGUNA LAYANAN OVER THE TOP COMMUNICATION SERVICES DI INDONESIA

Layanan Aplikasi Over The Top (OTT) sekarang ini tidak dapat lepas dari kehidupan setiap orang. Teknologi yang semakin canggih sehingga dapat mempermudah komunikasi jarak jauh membuat salah satu jenis OTT, yaitu layanan OTT communication services, seperti WhatsApp, digunakan masyarakat tanpa mengkhawatirkan risiko yang ada, yaitu penyalahgunaan data pribadi. Penyalahgunaan data pribadi merupakan bentuk pelanggaran atas hak privasi yang dapat dipertanggungjawabkan secara langsung kepada penyedia layanan OTT communication services sebagai pihak pengumpul data pribadi penggunanya. Namun, belum disahkannya rancangan undang-undang yang secara khusus mengatur mengenai perlindungan data pribadi di Indonesia menunjukkan bahwa pengguna OTT communication services belum mendapatkan perlindungan hukum yang memadai atas data pribadinya. Sedangkan, prinsip-prinsip penyelenggaraan data pribadi tidak dapat dijamin hanya oleh syarat dan ketentuan maupun kebijakan privasi layanan OTT communication services. Kata Kunci: Over The Top Communication Services, Data Pribadi, Hak Privasi, RUU Perlindungan Data Pribadi

L‐proline and betaine inhibit extracellular enzymes mediated abeta 1‐42 aggregation, oxidative stress, and toxicity

Extracellular amyloid plaques are characteristics of Alzheimer’s disease (AD). The amyloid plaques mainly consist of amyloid‐β (abeta) fragments. Extracellular biomolecules play a key role in abeta aggregation and subsequent plaque formation during AD. Among them, extracellular enzymes are known to induce abeta aggregation by catalyzing the crosslinking of abeta proteins. Although several therapeutic strategies have been studied to combat AD, studies to treat extracellular enzymes mediated abeta aggregation and toxicity is not explored in detail yet. Here we study the potential of osmolyte molecules l‐proline and betaine to inhibit the abeta aggregation and toxicity promoted by extracellular enzymes transglutaminase and lysyl oxidase in vitro. Results reveal that l‐proline and betaine have the potential to inhibit extracellular matrix enzymes mediated abeta aggregation/fibril formation and toxicity and oxidative stress in brain endothelial cells. Hence osmolytes could have the potential to modulate the effects of extracellular molecules during AD.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147177/1/pep224093_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/147177/2/pep224093.pd

GC-380 DineNGo Application

This project aims to upgrade the DineNGo application, a software tool for restaurant management, with new features specified by the client, The Driven Software Solutions. The upgraded version includes enhancements to users\u27 roles, such as implementing Role-Based Access Control (RBAC), and adding a payment required feature before order placement. Additionally, the new features aim to improve payment processing, reduce trips back and forth to the POS terminal, and provide a secure payment experience for customers. This document provides an overview of the project\u27s scope, objectives, and system overview, along with the operational policies and constraints

Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer

BACKGROUND: Several cell lines and primary cultures benefit from the use of positively charged extracellular matrix proteins or polymers that enhance their ability to attach to culture plates. Polyethyleneimine is a positively charged polymer that has gained recent attention as a transfection reagent. A less known use of this cationic polymer as an attachment factor was explored with several cell lines. RESULTS: Polyethyleneimine compared favorably to traditional attachment factors such as collagen and polylysine. PC-12 and HEK-293 cells plated on dishes coated with polyethyleneimine showed a homogeneous distribution of cells in the plate, demonstrating strong cell adhesion that survived washing procedures. The polymer could also be used to enhance the adherence and allow axonal outgrowth from zebrafish retinal explants. The effects of this coating agent on the transfection of loosely attaching cell lines were studied. Pre-coating with polyethyleneimine had the effect of enhancing the transfection yield in procedures using lipofection reagents. CONCLUSION: Polyethyleneimine is an effective attachment factor for weakly anchoring cell lines and primary cells. Its use in lipofection protocols makes the procedures more reliable and increases the yield of expressed products with commonly used cell lines such as PC-12 and HEK-293 cells

Characterisation, development and validation of UV Spectrophotometric technique for determining Diosmetin in bulk and nanoformulations

Background: From ancient times, India and many other countries have utilised natural products to manage various illnesses. Because they are abundantly available and have fewer adverse effects than synthetic medications, flavonoids are secondary metabolites with diverse biological activities. But till now, no formulations are developed by utilizing Diosmetin and no technique was formulated by utilizing self-biosynthesized Diosmetin (pure). It is a flavone (subclass of flavonoids) isolated from multiple medicinal plants but, in large amounts, is found in citrus plants. They have a variety of biological and pharmacological properties. They also improve lymphatic drainage by raising the duration and severity of lymphatic compression and the exact number of fully operational lymphatic capillaries. Methodology: We have procured Diosmetin from Otto Kemi Pvt. Ltd. and other chemicals from SIGMA chemicals. Furthermore, the characterisation of selected flavone has been performed through solubility studies and method development for development and validation by the UV method for nano-formulation. Result: Diosmetin is pure and soluble in various solvents and has a melting point between 259.14 and 261.84°Celsius, and is more soluble in Acetone, Chloroform, and DMSO, but we have utilised Diosmetin for Cancer studies, so we have taken it in 5% DMSO for further studies. Diosmetin is stable in DMSO till 20 mg/ml at room temperature

Review on Nanoparticles and Nanostructured Materials: Bioimaging, Biosensing, Drug Delivery, Tissue Engineering, Antimicrobial, and Agro-Food Applications

In the last few decades, the vast potential of nanomaterials for biomedical and healthcare applications has been extensively investigated. Several case studies demonstrated that nanomaterials can offer solutions to the current challenges of raw materials in the biomedical and healthcare fields. This review describes the different nanoparticles and nanostructured material synthesis approaches and presents some emerging biomedical, healthcare, and agro-food applications. This review focuses on various nanomaterial types (e.g., spherical, nanorods, nanotubes, nanosheets, nanofibers, core-shell, and mesoporous) that can be synthesized from different raw materials and their emerging applications in bioimaging, biosensing, drug delivery, tissue engineering, antimicrobial, and agro-foods. Depending on their morphology (e.g., size, aspect ratio, geometry, porosity), nanomaterials can be used as formulation modifiers, moisturizers, nanofillers, additives, membranes, and films. As toxicological assessment depends on sizes and morphologies, stringent regulation is needed from the testing of efficient nanomaterials dosages. The challenges and perspectives for an industrial breakthrough of nanomaterials are related to the optimization of production and processing conditions

Transfection yields were determined by β-galactosidase assays and normalized to the yields obtained with PEI coating of the culture dish (to which it was assigned an arbitrary value of 100

<p><b>Copyright information:</b></p><p>Taken from "Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer"</p><p>BMC Biotechnology 2004;4():23-23.</p><p>Published online 15 Oct 2004</p><p>PMCID:PMC526208.</p><p>Copyright © 2004 Vancha et al; licensee BioMed Central Ltd.</p>0%). PEI and other attachment factors enhanced the transfection of loosely attaching cell lines such as PC-12 cells (graph A) and HEK-293 cells (graph B). No significant effect was observed with tightly attaching cells such as MYS fibroblasts (graph C). The bars show the average ± standard deviation of triplicate assays, which are representative of at least two independent experiments (* significantly higher than untreated control, p < 0.01)