Transfection yields were determined by β-galactosidase assays and normalized to the yields obtained with PEI coating of the culture dish (to which it was assigned an arbitrary value of 100

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer"</p><p>BMC Biotechnology 2004;4():23-23.</p><p>Published online 15 Oct 2004</p><p>PMCID:PMC526208.</p><p>Copyright © 2004 Vancha et al; licensee BioMed Central Ltd.</p>0%). PEI and other attachment factors enhanced the transfection of loosely attaching cell lines such as PC-12 cells (graph A) and HEK-293 cells (graph B). No significant effect was observed with tightly attaching cells such as MYS fibroblasts (graph C). The bars show the average ± standard deviation of triplicate assays, which are representative of at least two independent experiments (* significantly higher than untreated control, p < 0.01)