EFFECTS OF MECHANICALLY GENERATED SLASH PARTICLE SIZE ON PRESCRIBED FIRE BEHAVIOR AND SUBSEQUENT VEGETATION EFFECTS

Forest managers have begun to restore ecosystem structure and function in fire-prone ecosystems that have experienced fire exclusion, commodity based resource extraction, and extensive grazing during much of the 20th century. Mechanical thinning and prescribed burning are the primary tools for thinning dense stands and restoring pre-settlement forest structure, reducing the likelihood of devastating crown fires. Mechanical thinning can be costly when trees are nonmerchantable and prescribed burning can be risky unless fuel loadings are first reduced. Furthermore, stands that remain dense after commercial thinning can produce undesirable wildland fire- or even prescribed fire- effects on vegetation and soils. Land managers are interested in using mastication equipment (Fig. 1) for thinning nonmerchantable trees as a means of restoring structure and function to dry forest ecosystems. However, it is unknown how the addition of mechanically derived slash influences potential fire behavior and fire effects. The objectives of this project were to test the effectiveness of mastication effort (defined as time needed to break fuels into smaller pieces) to 1) thin dense stands of dry coniferous forest within historically frequent, low-severity fire regimes (Fig. 1) and 2) create surface fuel beds that produce prescribed fire behavior with positive effects on residual trees, understory vegetation, and soils. Specifically, we asked the following questions: (1) How does slash particle size and fuel bed depth affect fire intensity and severity? (2) How do different mastication efforts and subsequent prescribed fire affect overstory vegetation? (3) Does soil heating change from burning different types of masticated slash? and (4) What are the differences in production costs among levels of mastication effort

Fungi Unearthed: Transcripts Encoding Lignocellulolytic and Chitinolytic Enzymes in Forest Soil

BACKGROUND: Fungi are the main organisms responsible for the degradation of biopolymers such as lignin, cellulose, hemicellulose, and chitin in forest ecosystems. Soil surveys largely target fungal diversity, paying less attention to fungal activity. METHODOLOGY/PRINCIPAL FINDINGS: Here we have focused on the organic horizon of a hardwood forest dominated by sugar maple that spreads widely across Eastern North America. The sampling site included three plots receiving normal atmospheric nitrogen deposition and three that received an extra 3 g nitrogen m(2) y(1) in form of sodium nitrate pellets since 1994, which led to increased accumulation of organic matter in the soil. Our aim was to assess, in samples taken from all six plots, transcript-level expression of fungal genes encoding lignocellulolytic and chitinolytic enzymes. For this we collected RNA from the forest soil, reverse-transcribed it, and amplified cDNAs of interest, using both published primer pairs as well as 23 newly developed ones. We thus detected transcript-level expression of 234 genes putatively encoding 26 different groups of fungal enzymes, notably major ligninolytic and diverse aromatic-oxidizing enzymes, various cellulose- and hemicellulose-degrading glycoside hydrolases and carbohydrate esterases, enzymes involved in chitin breakdown, N-acetylglucosamine metabolism, and cell wall degradation. Among the genes identified, 125 are homologous to known ascomycete genes and 105 to basidiomycete genes. Transcripts corresponding to all 26 enzyme groups were detected in both control and nitrogen-supplemented plots. CONCLUSIONS/SIGNIFICANCE: Many of these enzyme groups are known to be important in soil turnover processes, but the contribution of some is probably underestimated. Our data highlight the importance of ascomycetes, as well as basidiomycetes, in important biogeochemical cycles. In the nitrogen-supplemented plots, we have detected no transcript-level gap likely to explain the observed increased carbon storage, which is more likely due to community changes and perhaps transcriptional and/or post-transcriptional down-regulation of relevant genes

Inter- and intracisternal elements of the Golgi apparatus: A system of membrane-to-membrane cross-links

Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes of Clivia and Lilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternae as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes of Canna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex "zone of exclusion" which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types