Feasibility of restoring the Louisiana black bear (Ursus americanus luteolus) to portions of their former range

Black bears (Ursus americanus) historically occurred throughout much of North America’s forested landscapes, but loss of critical habitat and overharvest significantly reduced abundance and distribution. In the southeastern United States, black bear conservation has become a high priority and restoration has been suggested y to recolonize suitable habitat. My study focused on evaluating restoration of the Louisiana black bear (U. americanus luteolus) to the Red River Complex (RRC) in east-central Louisiana. This involved translocating female bears with new born cubs from source populations within Louisiana and monitoring their movements, habitat use, and social acceptance of the restoration program to determine if restoration attempts should be continued. Females relocated using this method had restricted home ranges and movements during the initial 30-days following release, and established home ranges at the release site within 7 months. Females continued to den in the area they established home ranges through the following 2 winters. Vegetation measurements at used locations suggest that habitat suitability in the RRC similar to other areas considered highly suitable for bears in the Southeast. A survey of hunters within the RRC indicates that support for the project was high (\u3e 70%) but knowledge about the restoration was low (\u3c 60%), although public meetings were held prior to the release of bears to the area. This data indicates that restoration of the Louisiana black bear to the RRC is feasible and should be continued in an effort to establish a new breeding sub-population of bears in the region

Serum Chemistry Values in Wild Black Vultures in Mississippi, USA

Vultures (Cathartidae and Accipitridae) play an important role in ecosystem balance by rapidly disposing animal carcasses and thus preventing the potential spread of pathogens. Blood chemistry values provide a means of assessing the health of wildlife and wild animal populations; however, there are significant differences in chemistries among species and when comparing captive and free-living New and Old World vultures. In 2007, we collected blood serum from 30 female and 14 male wild, healthy black vultures (Coragyps atratus) live-trapped by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services from a power substation in Lowndes County, Mississippi, USA. We analyzed the blood serum to provide serum chemistry base values for use in clinical pathology. The chemical analytes we measured included sodium, chloride, potassium, carbon dioxide, anion gap, glucose, creatinine, calcium, phosphorus, total protein, albumin, globulin, and aspartate aminotransferase. In general, blood chemistry values of black vultures were similar to those found in New and Old World vultures and raptor species. Average chemistry values for males were lower than females for sodium, chloride, creatinine, calcium, total protein, albumin, and globulin. The serum chemistry values we describe in this paper can be important indicators of avian health by gender for the black vulture. Our study provided important blood chemistry values from a large sample size, which is rarely available in free-ranging black vultures. These values could be used by scientists, veterinary pathologists, wildlife rehabilitation centers, and other researchers for baseline data for wild and free-ranging birds. Furthermore, the use of such parameters in assessing population health may enable conservationists to further research environmental conditions affecting species reproduction and survival

Serologic Evidence of Various Arboviruses Detected in White Tailed Deer (\u3ci\u3eOdocoileus virginianus\u3c/i\u3e) in the United States

White-tailed deer (Odocoileus virginianus) are an abundant mammal with a wide geographic distribution in the United States, which make them good sentinels for monitoring arboviral activity across the country. Exposure to various arboviruses has been detected in white-tailed deer, typically in conjunction with another diagnostic finding. To better assess the exposure of white-tailed deer to seven arboviruses, wetested 1,508 sera collected from 2010 to 2016 for antibodies to eastern equine encephalitis (2.5%), Powassan (4.2%), St. Louis encephalitis, (3.7%), West Nile (6.0%), Maguari (19.4%), La Crosse (30.3%), and bluetongue (7.8%) viruses. At least one arbovirus was detected in 51.3%, and exposure to more than one arbovirus was identified in 17.6% of the white-tailed deer sampled

Chapter 13: Development of a National Early Detection System for Highly Pathogenic Avian Influenza in Wild Birds in the United States of America

It is generally recognized that countries conducting comprehensive disease surveillance in wildlife populations are more likely to understand the epidemiology of specific infectious pathogens and zoonotic disease outbreaks (1, 2). These countries are better equipped and prepared to develop solutions that will protect humans, agriculture, and wildlife. Consequently, active surveillance for diseases of animal or public health concern in wildlife is particularly beneficial to national and international interests. The World Organization for Animal Health (OIE) encourages all countries to develop and maintain wildlife disease surveillance systems, which complement and support human health and agricultural animal disease programs. These systems should be capable of detecting unusual disease events, involve proficient diagnostic laboratories and international reference laboratories, use molecular characterization tools, and have communication protocols in place for rep0l1ing results (3). Disease surveillance systems should be designed to systematically collect relevant ecological and health-related data on the species of concern, efficiently assimilate and analyze data, and disseminate results of the analysis in an appropriate time frame to decision makers so that management actions can be rapidly implemented. It is this latter characteristic that distinguishes surveillance systems from the more commonly implemented monitoring systems in the study of wildlife diseases. Monitoring systems are developed to assess the health and disease status of a population and often are used to increase understanding of pathogens and their epidemiology in the environment. Alternatively, disease surveillance systems are more active by definition and incorporate thresholds for disease prevalence or incidence, above which, predefined management actions will be implemented (4, 5). Effective monitoring and surveillance systems (MOSS) form the foundation of all animal health programs and the most important component in strategies for preventing emerging infectious diseases

The Effect of Goose Management on Water Quality

Canada geese are causing a growing concern regarding their impact on public health and safety risks. In Pennsylvania, USDA Wildlife Services manages geese in problematic areas. The purpose of goose management is to reduce damage to agricultural, urban, and natural resources, as well as reducing threats to public health. For this study, three impoundments were monitored bi-weekly from May to September along with a single sampling date in both October and November 2009. Two of the impoundments were managed by the USDA, while the third was an unmanaged control site. The objective of the study was to compare water chemistry and fecal coliform counts from the three sites. Dissolved oxygen, pH, and water temperature were measured, along with fecal and total coliforms, to monitor water quality from the nesting to migration seasons. Results from fecal coliform testing show strong evidence for the benefits of management, with coliform levels up to 3 times higher in the unmanaged impoundment. Based on these findings, we conclude that USDA’s methods of management are effective in reducing health threats as well as improving water quality

Subtype-specific influenza A virus antibodies in Canada geese (\u3ci\u3eBranta canadensis\u3c/i\u3e)

Historically, surveillance for influenza A viruses (IAVs) in wild birds has relied on viral detection assays. This was largely due to poor performance of serological assays in wild birds; however, recently developed commercial serological assays have improved the ability to detect IAV antibodies in wild birds. Serological surveillance for IAV antibodies in Canada geese (Branta canadensis) has shown that, despite a low prevalence of virus isolations, Canada geese are frequently exposed to IAVs and that exposure increases with latitude, which follows virus isolation prevalence patterns observed in dabbling ducks. The objectives of this study were to further evaluate IAV antibodies in Canada geese using a subtype-specific serological assay to determine if Canada geese are exposed to subtypes that commonly circulate in dabbling ducks. We collected serum samples from Canada geese in Minnesota, New Jersey, Pennsylvania, and Wisconsin and tested for antibodies to IAVs using a blocking ELISA. Positive samples were further tested by hemagglutination inhibition for 10 hemagglutinin IAV subtypes (H1–H10). Overall, we detected antibodies to NP in 24% (714/2919) of geese. Antibodies to H3, H4, H5, and H6 subtypes predominated, with H5 being detected most frequently. A decrease in H5 HI antibody prevalence and titers was observed from 2009 to 2012. We also detected similar exposure pattern in Canada geese from New Jersey, Minnesota, Washington and Wisconsin. Based on the published literature, H3, H4, and H6 viruses are the most commonly reported IAVs from dabbling ducks. These results indicate that Canada geese also are frequently exposed to viruses of the same HA subtypes; however, the high prevalence of antibodies to H5 viruses was not expected as H5 IAVs are generally not well represented in reported isolates from ducks

Toxoplasma gondii prevalence in carnivorous wild birds in the eastern United States

Toxoplasma gondii is an important zoonotic protozoan parasite that can infect all warm-blooded animals including mammals and birds. Raptors can be intermediate hosts for T. gondii and the infection may be dependent on their feeding habits. In this study, we investigated the seroprevalence of T. gondii in ten raptor species from Florida, Pennsylvania, and Tennessee followed by a parasite bioassay on select seropositive samples. From a total of 155 raptors, we detected T. gondii antibodies using a modified agglutination test (cutoff 1:25) in 32 (20.6%) birds. The T. gondii seroprevalence was 44.8% in Falconiformes (13/29), 75% in Strigiformes (15/20), and 3.8% in Ciconiiformes (4/106). All Ciconiiformes samples (hearts and sera) were collected from Pennsylvania during nuisance wildlife removal projects and all birds were apparently healthy. Falconiform and Strigiform samples were collected from an exotics clinic in Tennessee and a rehabilitation center in Florida. All sampled birds were dead or euthanatized due to failure of rehabilitation or treatment. There was no statistically significant difference in T. gondii seroprevalence between Tennessee and Florida in the tested raptors. There was also no statistically significant difference in T. gondii exposure between males and females or adults and subadults. Mice bioassay attempts using fresh brain and/or heart tissue were performed on four seropositive birds. We isolated viable T. gondii tachyzoites from one red-shouldered hawk (Buteo lineatus) and genotyped the isolate using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of ten genetic markers. The isolated strain was designated as TgHawkFL1, which is ToxoDB PCR-RFLP genotype #28. Further research is needed to investigate the prevalence of T. gondii in raptors in the United States to obtain a better understanding of the life cycle, wildlife population impacts, and transmission dynamics of the parasite

Serologic Evidence of Various Arboviruses Detected in White Tailed Deer (\u3ci\u3eOdocoileus virginianus\u3c/i\u3e) in the United States

White-tailed deer (Odocoileus virginianus) are an abundant mammal with a wide geographic distribution in the United States, which make them good sentinels for monitoring arboviral activity across the country. Exposure to various arboviruses has been detected in white-tailed deer, typically in conjunction with another diagnostic finding. To better assess the exposure of white-tailed deer to seven arboviruses, wetested 1,508 sera collected from 2010 to 2016 for antibodies to eastern equine encephalitis (2.5%), Powassan (4.2%), St. Louis encephalitis, (3.7%), West Nile (6.0%), Maguari (19.4%), La Crosse (30.3%), and bluetongue (7.8%) viruses. At least one arbovirus was detected in 51.3%, and exposure to more than one arbovirus was identified in 17.6% of the white-tailed deer sampled

Antibodies to Avian Influenza Viruses in Canada Geese (\u3ci\u3eBranta canadensis\u3c/i\u3e): A Potential Surveillance Tool?

Traditionally, the epidemiology of avian influenza viruses (AIVs) in wild birds has been defined by detection of virus or viral RNA through virus isolation or reverse-transcription polymerase chain reaction. Our goals were to estimate AIV antibody prevalence in Canada geese (Branta canadensis) and measure effects of age and location on these estimates. We collected 3,205 samples from nine states during June and July 2008 and 2009: Georgia, Massachusetts, Minnesota, Mississippi, New Jersey, North Carolina, Pennsylvania, Washington, and West Virginia. Serum samples were tested for AIV antibodies with the use of a commercial blocking enzyme-linked immunosorbent assay. Overall, 483 (15%) Canada geese had detectable antibodies to AIV. Significantly higher prevalence’s were detected in geese collected from northeastern and upper midwestern states compared with southeastern states. This trend is consistent with results from virus isolation studies reporting AIV prevalence in North American dabbling ducks. Within Pennsylvania, significantly higher antibody prevalence’s were detected in goose flocks sampled in urban locations compared to flocks sampled in rural areas. Antibody prevalence was significantly higher in after-hatch-year geese compared to hatch-year geese. No significant differences in prevalence were detected from 10 locations sampled during both years. Results indicate that Canada geese are frequently exposed to AIVs and, with resident populations, may potentially be useful as sentinels to confirm regional AIV transmission within wild bird populations