Is genetic selection for skin nodule traits of ostriches feasible?

Preliminary genetic parameters for nodule traits of ostrich skins were estimated to examine whether genetic improvement of skin quality is feasible. Average nodule size and density per dmยฒ were determined on five localities on each of 439 ostrich skins. An animal model with random animal and skin permanent environmental effects was fitted for the traits considered. Fixed effects were locality on skin, age, year of hatch and gender. Locality significantly influenced nodule size and nodule density. Nodule size increased with an increase in slaughter age, while nodule density decreased. Estimates for heritability were low at 0.10 for both traits, but still differed significantly from zero. The genetic correlation between nodule density and average nodule size was very high at -0.72. The skin permanent environmental correlation was -0.33, the environmental correlation -0.17 and the phenotypic correlation was -0.28. More research into genetic variation within localities and correlations with other easily measurable traits is indicated for genetic improvement of ostrich leather quality. South African Journal of Animal Science Supp 2 2004: 29-3

Genetic variation in nodule size at different sites on the skins of slaughter ostriches

Nodule size is an important indicator of leather quality in the ostrich leather trade. The present study investigated genetic variation in nodule size at five sites on the skin, namely the neck, back, upper leg, flank and butt. Nodule size increased with an increased chronological age at all sites. Estimates of h² for nodule size ranged from 0.09 ± 0.07 on the flank region to 0.24 ± 0.10 on the upper leg region. Genetic correlations between nodule sizes measured at different sites were generally lower than expected, linked to high standard errors and, mostly not significant. These preliminary results seem to suggest that nodule size on different locations of the skin is not necessarily the same genetic trait. Apart from the limitations evident from these results, the objective measurement of nodules on ostrich skins is tedious when done manually, with little prospect for automation. The number of nodules per dm² (nodule density) was considered within skin sites as an indirect criterion for the improvement of nodule size. However, genetic correlations between nodule density and nodule size were negative, variable in size and generally not significantly different from zero or unity. Based on these preliminary results, alternative strategies for the genetic improvement of ostrich skin nodule size should be considered. South African Journal of Animal Science Vol. 36(3) 2006: 160-16

The influence of stocking rate and male:female ratio on the production of breeding ostriches (Struthio camelus spp.) under commercial farming conditions

The study provides information on the reproductive performance of ostriches maintained at different stocking rates and male:female (M:F) ratios under intensive commercial conditions in the Little Karoo, South Africa. Breeding ostriches are concentrated on relatively small areas and through trampling have a most significant impact on the vegetation in the Little Karoo. Reproductive performance, as influenced by stocking rate and M:F ratio, was investigated. Stocking rates for the large flocks ranged from 114 to 210 birds/ha, and stocking rates for smaller flocks ranged between 9 to 13 birds in 0.13 ha and 0.30 ha camps, respectively. The different M:F ratios investigated, were 1M:1F (pairs), 1M:2F (trios) and 1M:3F (quads), for breeding systems maintained in 0.06 ha camps. In almost all breeding systems total and average egg production, fertility and hatchability were compromised when stocking rate was increased. High stocking rates were detrimental to the reproductive performance and reproductive behaviour of the flocks. Increasing the number of females per male had no negative influence on the reproduction traits, with a significantly higher production observed for breeding quads. Our findings indicated that ostrich breeding flocks can be maintained at stocking rates higher than those presently used on commercial ostrich farms. Breeding pairs, trios and quads can also be maintained on smaller areas, with acceptable production levels. Increasing stocking densities will have a possible inhibitory effect on the establishment of territories and use of space, thereby impacting on the reproductive behaviour of ostrich females and males in large flocks, respectively. This has important implications in terms of the intensification of ostrich farming especially in areas that are characterised by vegetation that is exposed to the trampling effect of ostriches. South African Journal of Animal Science Vol.34(2) 2004: 87-9

Characterization of the commercially-available fluorescent chloroquine-BODIPY conjugate, LynxTag-CQGREEN, as a marker for chloroquine resistance and uptake in a 96-well plate assay

Chloroquine was a cheap, extremely effective drug against Plasmodium falciparum until resistance arose. One approach to reversing resistance is the inhibition of chloroquine efflux from its site of action, the parasite digestive vacuole. Chloroquine accumulation studies have traditionally relied on radiolabelled chloroquine, which poses several challenges. There is a need for development of a safe and biologically relevant substitute. We report here a commercially-available green fluorescent chloroquine-BODIPY conjugate, LynxTag-CQGREEN, as a proxy for chloroquine accumulation. This compound localized to the digestive vacuole of the parasite as observed under confocal microscopy, and inhibited growth of chloroquine-sensitive strain 3D7 more extensively than in the resistant strains 7G8 and K1. Microplate reader measurements indicated suppression of LynxTag-CQGREEN efflux after pretreatment of parasites with known reversal agents. Microsomes carrying either sensitive or resistant-type PfCRT were assayed for uptake; resistant-type PfCRT exhibited increased accumulation of LynxTag-CQGREEN, which was suppressed by pretreatment with known chemosensitizers. Eight laboratory strains and twelve clinical isolates were sequenced for PfCRT and Pgh1 haplotypes previously reported to contribute to drug resistance, and pfmdr1 copy number and chloroquine IC50s were determined. These data were compared with LynxTag-CQGREEN uptake/fluorescence by multiple linear regression to identify genetic correlates of uptake. Uptake of the compound correlated with the logIC50 of chloroquine and, more weakly, a mutation in Pgh1, F1226Y

Pervasive and opposing effects of Unpredictable Chronic Mild Stress (UCMS) on hippocampal gene expression in BALB/cJ and C57BL/6J mouse strains

Background: BALB/cJ is a strain susceptible to stress and extremely susceptible to a defective hedonic impact in response to chronic stressors. The strain offers much promise as an animal model for the study of stress related disorders. We present a comparative hippocampal gene expression study on the effects of unpredictable chronic mild stress on BALB/cJ and C57BL/6J mice. Affymetrix MOE 430 was used to measure hippocampal gene expression from 16 animals of two different strains (BALB/cJ and C57BL/6J) of both sexes and subjected to either unpredictable chronic mild stress (UCMS) or no stress. Differences were statistically evaluated through supervised and unsupervised linear modelling and using Weighted Gene Coexpression Network Analysis (WGCNA). In order to gain further understanding into mechanisms related to stress response, we cross-validated our results with a parallel study from the GENDEP project using WGCNA in a meta-analysis design. Results: The effects of UCMS are visible through Principal Component Analysis which highlights the stress sensitivity of the BALB/cJ strain. A number of genes and gene networks related to stress response were uncovered including the Creb1 gene. WGCNA and pathway analysis revealed a gene network centered on Nfkb1. Results from the meta-analysis revealed a highly significant gene pathway centred on the Ubiquitin C (Ubc) gene. All pathways uncovered are associated with inflammation and immune response. Conclusions: The study investigated the molecular mechanisms underlying the response to adverse environment in an animal model using a GxE design. Stress-related differences were visible at the genomic level through PCA analysis highlighting the high sensitivity of BALB/cJ animals to environmental stressors. Several candidate genes and gene networks reported are associated with inflammation and neurogenesis and could serve to inform candidate gene selection in human studies and provide additional insight into the pathology of Major Depressive Disorder

Evaluation of subjectively assessed nodule traits of ostrich skins as influenced by slaughter age

No Abstract. South African Journal of Animal Science Vol. 35 (1) 2005: pp.48-5

The effect of temperature on the hatching performance of ostrich chicks, and its implications for artificial incubation in forced draught wooden incubators

No Abstract

Is genetic selection for skin nodule traits of ostriches feasible?

AgriwetenskappeVeekundige WetenskappePlease help us populate SUNScholar with the post print version of this article. It can be e-mailed to: [email protected]

Effects of age on leather and skin traits of slaughter ostriches.

AgriwetenskappeVeekundige WetenskappePlease help us populate SUNScholar with the post print version of this article. It can be e-mailed to: [email protected]