634 research outputs found
Expression and characterization of the product of a human immune interferon cDNA gene in Chinese hamster ovary cells.
X-ray spectral imaging and Doppler mapping of Cassiopeia A
A detailed X-ray spectral analysis of Cas A using a deep exposure from the
EPIC-MOS cameras on-board XMM-Newton is presented. Spectral fitting was
performed on a 15x15 grid of 20"x20" pixels using a two component
non-equilibrium ionisation model (NEI) giving maps of ionisation age,
temperature, interstellar column density, abundances and Doppler velocities.
The abundances of Si, S, Ar and Ca are strongly correlated. The abundance
ratios are consistent with the nucleosynthesis yield from the collapse of a 12
Msun progenitor. The abundance ratios Ne/Si, Mg/Si, Fe/Si and Ni/Si are very
variable and distinctly different from S/Si, Ar/Si and Ca/Si, in line with the
current explosive nucleosynthesis models. The ionisation age and temperature of
both NEI components varies considerably over the remnant. Accurate
determination of these parameters yield reliable Doppler velocities for both
components. The data are consistent with a plasma velocity of 2600 km/s at the
shock radius of 153" implying a primary shock velocity of 4000+/-500 km/s. The
Si-K and S-K line emission from the cool component is confined to a relatively
narrow shell with radius 100-150". This component is almost certainly ejecta
material which has been heated by a combination of the reverse shock and
heating of ejecta clumps as they plough through the medium which has been
pre-heated by the primary shock. The Fe-K line emission is expanding faster and
spans a radius range 110-170". The bulk of the Fe emission is confined to two
large clumps and it is likely that these too are the result of ablation from
ejecta bullets rather swept up circumstellar medium.Comment: 10 pages, 11 figures, submitted to Astronomy and Astrophysic
Measuring the cosmic ray acceleration efficiency of a supernova remnant
Cosmic rays are the most energetic particles arriving at earth. Although most
of them are thought to be accelerated by supernova remnants, the details of the
acceleration process and its efficiency are not well determined. Here we show
that the pressure induced by cosmic rays exceeds the thermal pressure behind
the northeast shock of the supernova remnant RCW 86, where the X-ray emission
is dominated by synchrotron radiation from ultra-relativistic electrons. We
determined the cosmic-ray content from the thermal Doppler broadening measured
with optical spectroscopy, combined with a proper-motion study in X- rays. The
measured post-shock proton temperature in combination with the shock velocity
does not agree with standard shock heating, implying that >50% of the
post-shock pressure is produced by cosmic rays.Comment: Published in Science express, 10 pages, 5 figures and 2 table
The mass and energy budget of Cassiopeia A
Further analysis of X-ray spectroscopy results recently obtained from the MOS
CCD cameras on-board XMM-Newton provides a detailed description of the hot and
cool X-ray emitting plasma in Cas A. Measurement of the Doppler broadening of
the X-ray lines is consistent with the expected ion velocities, ~1500 km/s
along the line of sight, in the post shock plasma. Assuming a constant total
pressure throughout the remnant we estimate the total remnant mass as 10 Msun
and the total thermal energy as 7E43 J. We derive the differential mass
distribution as a function of ionisation age for both X-ray emitting
components. This distribution is consistent with a hot component dominated by
swept up mass heated by the primary shock and a cool component which are
ablated clumpy ejecta material which were and are still being heated by
interaction with the preheated swept up material. We calculate a balanced mass
and energy budget for the supernova explosion giving 1E44 J in ejected mass;
approximately 0.4 Msun of the ejecta were diffuse with an initial rms velocity
of 15000 km/s while the remaining ~1.8 Msun were clumpy with an initial rms
velocity of ~2400 km/s. Using the Doppler velocity measurements of the X-ray
spectral lines we can project the mass into spherical coordinates about the
remnant. This provides quantitative evidence for mass and energy beaming in the
supernova explosion. The mass and energy occupy less than 4.5 sr (<40 % of the
available solid angle) around the remnant and 64 % of the mass occurs in two
jets within 45 degrees of a jet axis. We calculate a swept up mass of 7.9 Msun
in the emitting plasma and estimate that the total mass lost from the
progenitor prior to the explosion could be as high as ~20 Msun.Comment: 8 pages, 7 figures, submitted to Astronomy & Astrophysic
Experimental Mapping of the Canine KCNJ2 and KCNJ12 Gene Structures and Functional Analysis of the Canine KIR2.2 ion Channel
For many model organisms traditionally in use for cardiac electrophysiological studies, characterization of ion channel genes is lacking. We focused here on two genes encoding the inward rectifier current, KCNJ2 and KCNJ12, in the dog heart. A combination of RT-PCR, 5′-RACE, and 3′-RACE demonstrated the status of KCNJ2 as a two exon gene. The complete open reading frame (ORF) was located on the second exon. One transcription initiation site was mapped. Four differential transcription termination sites were found downstream of two consensus polyadenylation signals. The canine KCNJ12 gene was found to consist of three exons, with its ORF located on the third exon. One transcription initiation and one termination site were found. No alternative splicing was observed in right ventricle or brain cortex. The gene structure of canine KCNJ2 and KCNJ12 was conserved amongst other vertebrates, while current GenBank gene annotation was determined as incomplete. In silico translation of KCN12 revealed a non-conserved glycine rich stretch located near the carboxy-terminus of the KIR2.2 protein. However, no differences were observed when comparing dog with human KIR2.2 protein upon ectopic expression in COS-7 or HEK293 cells with respect to subcellular localization or electrophysiological properties
Dog KCNJ2 and KCNJ12 genes
For many model organisms traditionally in use for cardiac electrophysiological studies, characterization of ion channel genes is lacking. We focused here on two genes encoding the inward rectifier current, KCNJ2 and KCNJ12, in the dog heart. A combination of RT-PCR, 5′-RACE, and 3′-RACE demonstrated the status of KCNJ2 as a two exon gene. The complete open reading frame (ORF) was located on the second exon. One transcription initiation site was mapped. Four differential transcription termination sites were found downstream of two consensus polyadenylation signals. The canine KCNJ12 gene was found to consist of three exons, with its ORF located on the third exon. One transcription initiation and one termination site were found. No alternative splicing was observed in right ventricle or brain cortex. The gene structure of canine KCNJ2 and KCNJ12 was conserved amongst other vertebrates, while current GenBank gene annotation was determined as incomplete. In silico translation of KCN12 revealed a non-conserved glycine rich stretch located near the carboxy-terminus of the KIR2.2 protein. However, no differences were observed when comparing dog with human KIR2.2 protein upon ectopic expression in COS-7 or HEK293 cells with respect to subcellular localization or electrophysiological properties
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