Species identification of freshwater crab in Jajroud River

Jajroud river is one of the most important aquatic ecosystem in Iran (east of Tehran) and consist of variety of different aquatics, of which is a freshwater crab. The crab sample were collected from 5 stations of Taraghion, Khojeer, Latian, Daroee plants and Mamloo dam. The collected samples were studied from point of reproduction and feeding behaviour; and also, carapace length, carapace width and body weight were measured. The most important objective of this survey was to find out the precise species identification. In this regard, sample were transported to the Natural History Museum in Netherland and ultimately, it was certified that all specimens belong to Potamidae family and species of Potamon persicum

Natural feeding of johns snapper (Lutjanus johnii) in the Persian Gulf (Hormuzgan Province waters) Iran

Since a little information is available in relation with feeding of Lutjanus johni, this study was conducted to determine its food diversity. 613 specimens were studied from May 1996 to July 1997 and their dietary regim was defined. In this study, capacity vacuous index (CV) and food preference (Fp) calculated for this fish. The study of stomachs showed that stomach of 44 fish were full, 27 semifull and 524 were empty. CV was obtained 88.4 and Fp for crustacean (55.6), fishes (44.3) and molusca (1.4). In particularly study, Fp calculated for crab (54.0), squilla (23.2), shrimp (20.2) and lobster (2.5). Ultimately, in this survey was found that this fish possibly is fast feeder and crustacean to be omposed their main food. Among crustacean, crab is main food

Morphometric study on Sepia pharaonis in the Persian Gulf and Oman Sea

The pharaoh cuttlefish (Sepia pharaonis) is the most aboundant species in the Persian Gulf and Oman Sea. The stock patterns of this species were studied concerning biological and morphological aspects. In addition to measuring the biological patterns, 20 quantitative factors were measured. The results of LSD test showed that tentacle organ is indicative factor which shows significant difference between male and female specimen of Bushehr and Balouchestan regions. Regarding to length and weight frequencies data the results indicated that males are always bigger than females and also, the cuttlefishes of the Gulf of Oman are bigger than Persian Gulf samples. The results of cluster analysis for both sexes showed that the cuttlefishes of both studied region belong to separate stocks. Taking into consideration the findings of present study including: (1) difference in spawning season, (2) results of dendrograms, (3) observed significant differences in one-way analysis of variance (ANOVA) for morphometric measurements, (4) differences in body length and weight, (5) as well as ecological variations of the Persian Gulf and "the pharaoh cuttlefishes of Bushehr and Oman Sea, have indicated that Balouchestan belong to two separate stocks

Study on fauna and flora community of the Iranian coastal zones

The report provides comprehensive information and exclusive native species and their status in terms of protection, browse and view Resorts® reserve forest vegetation and coastal areas of the Persian Gulf and Oman Sea and Caspian Sea is mentioned. In a series of studies including field trips, collect and identify plants in Iran, the list has been presented since 1362 to date in the form of plans and programs identify plants is planned. It should be noted that all identified studies and knowledge of plants and notes as well as an identification key based on the information contained in Flora Iranica valuable book written K.H. Rechinger plays a key role and is essential to identify the plants. along with scientific names, synonyms and names mentioned Farsi and English with the geographic distribution is presented according to two area Shmnaly and south. Based on existing resources, conservation status of species under national legislation and international and native status (Endemic) and biological values and biodiversity of geographically referenced

Estimation of MSY on six species of commercially important demersal fishes in the Persian Gulf & Oman Sea (Hormuzgan province)

Today, calculation of MSY is one of the necessary fisheries management in control and prevention of the fish population reduction and is obtained with different methods. This study has focused on six species of commercial fish, including Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot, Yellowfin seabream and Silver pomfret. The study was done monthly, from January 2007 to March 2008, in three fish landing regions including: Bandar Lengeh, Bandar Abbas and Qeshm Island (Slakh, Basydu and Chahoshrqy). Total 5163 Silver pomfret (Pompus argenteus), 1766 Javelin grunter (Pomadasys kaakan), 2151 John`s snapper (Lutjanus johnii), 3280 Tiger-toothed croaker (Otolithes ruber), 1628 Indian spiny turbot (Psettodes erumei) and the number of 759 Yellowfin seabream (Acnthopagrus latus) were assessed and length biometry has been done, monthly. In this study, two methods were used to determine the maximum sustainable yield (MSY): 1- virtual population analysis (Cohort analysis) 2- use of statistics and information that was estimated with two method, catch prediction and biomass (Standing stock). The results showed that in 2007, MSY value was estimated through catch prediction for Silver pomfret, Tigertoothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 1354, 1116, 1099.6, 1045.5, 914.5 and 529.5 tons, respectively. Moreover, this estimation have been done through standing stock for Silver pomfret, Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 1215, 633, 1304, 878, 1095 and 441 tons, respectively; and through VPA for Silver pomfret, Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 1100, 850, 920, 732.5, 1002.3 and 403 tons, respectively. Amount of biomass (Standing Stock) was estimated for Silver pomfret, Tiger-toothed croaker, Javelin grunter, John`s snapper, Indian spiny turbot and Yellowfin seabream 2530, 1172, 1738, 1689, 1470 and 1110 tons, respectively. In general, by assessing the obtained results for the studied species, except the fishing prediction results of the Javelin grunter and Tiger-toothed croaker species, which is less valuable due to the low correlation coefficient, it can be stated that except fishing pressure on John`s snapper and Tiger-toothed croaker, in other species studied, fishing conditions are in optimal situation

Gene bank preparation from the endemic shrimps and crustaceans of Persian Gulf and Oman Sea

Genetic knowledge and Gene bank preparation can help to protect biodiversity and detect , species identify , fishing offenses , genetic classification and also identification the faliure cross hybridizations of marine animals. In this study, sampling was performed from Jask, guatr and Hormuz areas, which is the most important habitats for the species studied using bottom trawl. Total DNA extraction was performed using phenol- chloroform method. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program. Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA and COI gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 48 and 54 Celsius degrees respectively. The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA and COI, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 5 species of shrimp (P. semisulcatus، P. indicus، P. merguiensis ، P. monodon، M. affinis) in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank. Molecular investigation of mitochondria DNA (mtDNA) using partial sequences of 16S rRNA gene showed relatively low genetic differences between the P. semisulcatus morphotypes. These sequences were able to distinguish between the two morphotypes, and separated them into two distinct clades. Also genetic divergence detected by COI gene analysis was consistently higher. High genetic divergence for COI was observed between the two morphotypes of P.semeisulcatus which emphesise that the gene bank preparation should be perform for this morphotype of this species. This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. In this case, different management in broodstocking programs should be performed for two morphotypes of P. semisulcatus which were detected in Persian Gulf. The results of this study show that two Morphotype of P.semisulcatus can be considered as two separate species from genetic aspects. In this regard, it can be assumed that the genetic composition of the studied species is very close together and we've no seen a huge difference in the species except in the green tiger species

Population genetic study on croaker species Atrobucca nibe in Persian Gulf and Oman Sea using molecular methods

Black Mouth Croaker (Atrobucca nibe) is of significant value from the economics point of view. In the following study, the genetic diversity of Atrobucca nibe has been investigated using the PCR-sequencing method from three different regions of the Persian gulf and Oman Sea, namely Chabahar in the Sistan and Baluchestan and Jask in Hormozgan Province of Iran and Fujairah in the Fujairah Emirate of the United Arab of Emirates. The purpose of the following exploration has essentially beencoined to further the study of Atrobucca nibe genetic diversity and its population structure in the Persian Gulf and Oman Sea. For this purpose, a number of 54 fish have been collected from the aforementioned regions. The DNA of the fish had been extracted through the Ammonium Acetate method and the quality of the DNAs were analyzed via the spectrophotometry and furthermore the quantity of the extracted DNA were assessed viaelectrophoresis. Polymerase Chain Reaction (PCR) was conducted on the targeted DNAs and thenceforth DNA sequencing was carried out. Subsequently, through sequencing it was discovered that the D-loop region in the mitochondrial DNA (mtDNA) of Black Mouth Croaker,contained 270 base pairs (bp). For the purpose of analyzing the genetic diversity of Atrobucca nibe and its population structure in the Persian Gulf and Oman Sea, the following softwares have been conducted, namely theBioEdit, Arlequin, Dnasp and Mega5 software. Furthermore, the Kimura 2-parameter was used for the purpose of genetic distance analysis. Through observations of average levels of FST between the regions, it can be summed up that genetic differences do exist among the present populations. On the grounds of the obtained results from the Persian Gulf and Oman Sea, it can be concluded that there currently are three different populations of Atrobucca nibe residing in the waters of Sistan & Baluchestan, Hormozgan and Fujairah

Study forming of growth rings in the Caspian bony fishes (Step 1: Common carp Cyprinus carpio Linnaeus, 1758)

The ichthyology studies at fish tagging was referred to more than 100 years ago. Tagging is utilized for the estimation of stock amount, population frequency and project or calculating of survival and growth value. There are many various methods for fish tagging are used for juvenile to adult fish. The current study was carried out at Ghare-su research station during 2011-2013. 250 of carp fingerlings (Cyprinus carpio) were tagged by bath with tetracycline manner (pH=6, 500mg/l). The fork length and weight of fish were 10-12 mm and 2-4 g, respectively. Also, fish were fed twice a day. The second and third tagging stages were performed at November 2012 and July 2014, respectively. After otolith was extracted, it was washed water and dry at lab room air. The tagged rings or loops (annuali) were observed by lab loupe with 10X and 40 X magnifications. The results showed that a narrow band was formed after the first tagging of carp fingerlings, as it was seen at the end of otolith edge. It was demonstrated that tetracycline was absorbed and the tagged ring or loop (annuli) was newly formed at external or out of othlith edge. There was not observed any fish death, when carp fingerlings were tagged by OTC bath manner. It said that the first OTC band will be seen, the first sign of the formed OTC band was observed at external margin of otoilith after 6th month , of course , from 4th month, this sign was formed at the most external of otoilth edge but with added the following region, it can be hardly distinguished. The formed band in relative to second tagging was similar to the first band but there was no discrepancy or difference with together