Identification of Novel Methane-, Ethane-, and Propane-Oxidizing Bacteria at Marine Hydrocarbon Seeps by Stable Isotope Probing

Marine hydrocarbon seeps supply oil and gas to microorganisms in sediments and overlying water. We used stable isotope probing (SIP) to identify aerobic bacteria oxidizing gaseous hydrocarbons in surface sediment from the Coal Oil Point seep field located offshore of Santa Barbara, California. After incubating sediment with ^(13)C-labeled methane, ethane, or propane, we confirmed the incorporation of ^(13)C into fatty acids and DNA. Terminal restriction fragment length polymorphism (T-RFLP) analysis and sequencing of the 16S rRNA and particulate methane monooxygenase (pmoA) genes in ^(13)C-DNA revealed groups of microbes not previously thought to contribute to methane, ethane, or propane oxidation. First, ^(13)C methane was primarily assimilated by Gammaproteobacteria species from the family Methylococcaceae, Gammaproteobacteria related to Methylophaga, and Betaproteobacteria from the family Methylophilaceae. Species of the latter two genera have not been previously shown to oxidize methane and may have been cross-feeding on methanol, but species of both genera were heavily labeled after just 3 days. pmoA sequences were affiliated with species of Methylococcaceae, but most were not closely related to cultured methanotrophs. Second, ^(13)C ethane was consumed by members of a novel group of Methylococcaceae. Growth with ethane as the major carbon source has not previously been observed in members of the Methylococcaceae; a highly divergent pmoA-like gene detected in the ^(13)C-labeled DNA may encode an ethane monooxygenase. Third, ^(13)C propane was consumed by members of a group of unclassified Gammaproteobacteria species not previously linked to propane oxidation. This study identifies several bacterial lineages as participants in the oxidation of gaseous hydrocarbons in marine seeps and supports the idea of an alternate function for some pmoA-like genes

Food safety

Illness induced by unsafe food is a problem of great public health significance. This study relates exclusively to the occurrence of chemical agents which will result in food unsafe for human consumption since the matter of food safety is of paramount importance in the mission and operation of the manned spacecraft program of the National Aeronautics and Space Administration

Application of cabin atmosphere monitors to rapid screening of breath samples for the early detection of disease states

Analysis of human breath is a nonintrusive method to monitor both endogenous and exogenous chemicals found in the body. Several technologies were investigated and developed which are applicable to monitoring some organic molecules important in both physiological and pathological states. Two methods were developed for enriching the organic molecules exhaled in the breath of humans. One device is based on a respiratory face mask fitted with a polyethylene foam wafer; while the other device is a cryogenic trap utilizing an organic solvent. Using laboratory workers as controls, two organic molecules which occurred in the enriched breath of all subjects were tentatively identified as lactic acid and contisol. Both of these substances occurred in breath in sufficient amounts that the conventional method of gas-liquid chromatography was adequate for detection and quantification. To detect and quantitate trace amounts of chemicals in breath, another type of technology was developed in which analysis was conducted using high pressure liquid chromatography and mass spectrometry

Oceanographic data from the R.R.S. Discovery II. : International Geophysical Year cruise 3, 1958

Originally issued as Reference No. 59-54In August and September 1958 a number of oceanographic sections were made in the R.R.S. DISCOVERY II, Captain James Gray, under the scientific leadership of H. F. P. Herdman. These sections comprise part of a joint program undertaken by the National Institute of Oceanography and the Woods Hole Oceanographic Institution for the International Geophysical Year.The United States participation in this program was supported by the Office of Naval Research Contract Nonr 2196(00) with the Woods Hole Oceanographic Institution

Examining Bridges Between Informal and Formal Learning Environments: A Sequential Mixed Method Design

The purpose of this sequential mixed method study was to identify schools implementing a technology-based engineering design intervention in a way that connects or bridges formal learning environments of the school-day to informal learning environments such as afterschool programs. Further, this study investigated educators’ decisions that enabled or facilitated bridging between formal and informal learning environments. This cooperation and/or linking between informal and formal learning time is bridging. Participants included public schools (n=16) in Eastern Nebraska that incorporated the Nebraska Wearables Technology (WearTec) program at their school, club or Out-of-School-Time program during the 2015-2016 school year. Three of the schools bridged formal and informal environments. For this study descriptive statistics were used to analyze the implementation of the WearTec curriculum and as a means to select schools which bridged the formal and informal learning environments. Interviews with a priori codes and thematic analysis were analyzed in a matching/exploratory case study of the schools that bridged formal and informal learning environments (n=3). Thematic and descriptive analysis of interviews suggests a pair of educators can create a bridge due to the WearTec curriculum, state standards, and interpersonal communication. Also, a single formal day teacher can create a bridge by creating informal learning opportunities in out-of-school time. Advisor: Bradley Barke

Interview with Barry Valentine by Andrea L’Hommedieu

Biographical NoteBarry Lee Valentine was born September 12, 1943, in Emporia Kansas. He grew up in York Harbor, Maine, attended York High School, and received a degree in engineering from Rensselaer Polytechnic Institute. During college he took part in ROTC and after graduation joined the Air Force, serving as a pilot in Vietnam. He left the Air Force in 1972 and returned to Maine, where he helped run an airfield and became involved in politics because a neighbor ran for a seat in the Maine state legislature. He was the York county coordinator for the Maine public power campaign and then joined George Mitchell’s 1974 gubernatorial primary campaign, serving as a scheduler and driver. That fall he ran unsuccessfully for state Senate. He worked in Augusta, Maine, as a staffer for the state House majority leader. In 1976 he made a successful bid for a seat in the state legislature. In 1979 he became a district manager for the 1980 census. He worked in aviation, managing the Portland Jet Port, serving as the regional vice chairman of the National Association of State Aviation Officials, and as chairman of the Airports Committee. Then in 1992 he began work as a staffer for Senate Majority Leader Mitchell on the Senate Select Committee on POW/MIA Affairs. After the committee completed its work, Valentine became an administrator at the FAA during the Clinton administration. At the time of this interview he was semi-retired. SummaryInterview includes discussion of: family and educational background; Valentine’s love of flying; Air Force and Vietnam; working on Neil Rolde’s 1972 campaign; the Maine public power referendum; working for George Mitchell in the 1974 gubernatorial campaign; an anecdote about a mill worker who wanted to know what Mitchell would ‘do about them women’ who were getting jobs on the mill floor; Barry’s insights as scheduler/driver; Mitchell’s position on two Democratic planks – gay rights and amnesty for those who avoided the draft – and an anecdote explaining his position to four truckers at a Bangor truck stop; Barry’s running for state senate in 1974 and winning election to the state legislature in 1976; the 1980 census; Mitchell’s 1982 campaign; working on the Senate Select Committee on POW/MIA Affairs; the 1992 presidential election; and Mitchell’s commitment to his beliefs even when they were politically unpopular

Examining Bridges Between Informal and Formal Learning Environments: A Sequential Mixed Method Design

The purpose of this sequential mixed method study was to identify schools implementing a technology-based engineering design intervention in a way that connects or bridges formal learning environments of the school-day to informal learning environments such as afterschool programs. Further, this study investigated educators’ decisions that enabled or facilitated bridging between formal and informal learning environments. This cooperation and/or linking between informal and formal learning time is bridging. Participants included public schools (n=16) in Eastern Nebraska that incorporated the Nebraska Wearables Technology (WearTec) program at their school, club or Out-of-School-Time program during the 2015-2016 school year. Three of the schools bridged formal and informal environments. For this study descriptive statistics were used to analyze the implementation of the WearTec curriculum and as a means to select schools which bridged the formal and informal learning environments. Interviews with a priori codes and thematic analysis were analyzed in a matching/exploratory case study of the schools that bridged formal and informal learning environments (n=3). Thematic and descriptive analysis of interviews suggests a pair of educators can create a bridge due to the WearTec curriculum, state standards, and interpersonal communication. Also, a single formal day teacher can create a bridge by creating informal learning opportunities in out-of-school time. Advisor: Bradley Barke

Diversity and N2O Production Potential of Fungi in an Oceanic Oxygen Minimum Zone

Fungi in terrestrial environments are known to play a key role in carbon and nitrogen biogeochemistry and exhibit high diversity. In contrast, the diversity and function of fungi in the ocean has remained underexplored and largely neglected. In the eastern tropical North Pacific oxygen minimum zone, we examined the fungal diversity by sequencing the internal transcribed spacer region 2 (ITS2) and mining a metagenome dataset collected from the same region. Additionally, we coupled 15N-tracer experiments with a selective inhibition method to determine the potential contribution of marine fungi to nitrous oxide (N2O) production. Fungal communities evaluated by ITS2 sequencing were dominated by the phyla Basidiomycota and Ascomycota at most depths. However, the metagenome dataset showed that about one third of the fungal community belong to early-diverging phyla. Fungal N2O production rates peaked at the oxic–anoxic interface of the water column, and when integrated from the oxycline to the top of the anoxic depths, fungi accounted for 18–22% of total N2O production. Our findings highlight the limitation of ITS-based methods typically used to investigate terrestrial fungal diversity and indicate that fungi may play an active role in marine nitrogen cycling

Predicting Mortality in Children With Pediatric Acute Respiratory Distress Syndrome: A Pediatric Acute Respiratory Distress Syndrome Incidence and Epidemiology Study

OBJECTIVES: Pediatric acute respiratory distress syndrome is heterogeneous, with a paucity of risk stratification tools to assist with trial design. We aimed to develop and validate mortality prediction models for patients with pediatric acute respiratory distress syndrome. DESIGN: Leveraging additional data collection from a preplanned ancillary study (Version 1) of the multinational Pediatric Acute Respiratory Distress syndrome Incidence and Epidemiology study, we identified predictors of mortality. Separate models were built for the entire Version 1 cohort, for the cohort excluding neurologic deaths, for intubated subjects, and for intubated subjects excluding neurologic deaths. Models were externally validated in a cohort of intubated pediatric acute respiratory distress syndrome patients from the Children\u27s Hospital of Philadelphia. SETTING: The derivation cohort represented 100 centers worldwide; the validation cohort was from Children\u27s Hospital of Philadelphia. PATIENTS: There were 624 and 640 subjects in the derivation and validation cohorts, respectively. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: The model for the full cohort included immunocompromised status, Pediatric Logistic Organ Dysfunction 2 score, day 0 vasopressor-inotrope score and fluid balance, and PaO2/FIO2 6 hours after pediatric acute respiratory distress syndrome onset. This model had good discrimination (area under the receiver operating characteristic curve 0.82), calibration, and internal validation. Models excluding neurologic deaths, for intubated subjects, and for intubated subjects excluding neurologic deaths also demonstrated good discrimination (all area under the receiver operating characteristic curve \u3e /= 0.84) and calibration. In the validation cohort, models for intubated pediatric acute respiratory distress syndrome (including and excluding neurologic deaths) had excellent discrimination (both area under the receiver operating characteristic curve \u3e /= 0.85), but poor calibration. After revision, the model for all intubated subjects remained miscalibrated, whereas the model excluding neurologic deaths showed perfect calibration. Mortality models also stratified ventilator-free days at 28 days in both derivation and validation cohorts. CONCLUSIONS: We describe predictive models for mortality in pediatric acute respiratory distress syndrome using readily available variables from day 0 of pediatric acute respiratory distress syndrome which outperform severity of illness scores and which demonstrate utility for composite outcomes such as ventilator-free days. Models can assist with risk stratification for clinical trials