Atividade inseticida de óleos essenciais de Pelargonium graveolens l'Herit e Lippia alba (Mill) N. E. Brown sobre Spodoptera frugiperda (J. E. Smith)

Insecticidal activity of essential oils of Pelargonium graveolens, Lippia alba and compounds geraniol, linalool, 1,8-cineole, limonene, carvone, citral and Azamax® were evaluated against Spodoptera frugiperda. Topical application assay showed essential oil of P. graveolens has acute toxicity against Spodoptera frugiperda larvae (third instar) with LD50 1.13 µg/mg per insect and LD90 2.56 µg/mg per insect. Three essential oils of L. alba also exhibited insecticidal activity with LD50 ranging from 1.20 to 1.56 µg/mg per insect and LD90 from 2.60 to 3.75 µg/mg per insect. Geraniol, linalool, carvone and citral caused significant mortality of 30, 90, 84 and 64% respectively, compared to negative control. The bioinsecticide, Azamax®, caused lower mortality than the compounds of the essential oils

Cytotoxic effect of leaf essential oil of Lippia gracilis Schauer (Verbenaceae).

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-10-08T18:38:47Z No. of bitstreams: 1 Ferraz RPC Cytotoxic effect....pdf: 518077 bytes, checksum: 7e9ab563e64c540459b870f256a3843a (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2014-10-08T18:38:57Z (GMT) No. of bitstreams: 1 Ferraz RPC Cytotoxic effect....pdf: 518077 bytes, checksum: 7e9ab563e64c540459b870f256a3843a (MD5)Made available in DSpace on 2014-10-08T18:53:10Z (GMT). No. of bitstreams: 1 Ferraz RPC Cytotoxic effect....pdf: 518077 bytes, checksum: 7e9ab563e64c540459b870f256a3843a (MD5) Previous issue date: 2013Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Physiology. São Cristóvão, SE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Biology. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Biology. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Chemistry. São Cristóvão, SE, BrasilFederal University of Sergipe. Department of Physiology. São Cristóvão, SE, BrasilMedicinal plants are one of the most important sources of drugs used in the pharmaceutical industry. Among traditional medicinal plants, Lippia gracilis Schauer (Verbenaceae) had been used for several medicinal purposes in Brazilian northeastern. In this study, leaf essential oil (EO) of L. gracilis was prepared using hydrodistillation. Followed by GC–MS analysis, its composition was characterized by the presence of thymol (55.50%), as major constituent. The effects of EO on cell proliferation and apoptosis induction were investigated in HepG2 cells. Furthermore, mice bearing Sarcoma 180 tumor cells were used to confirm its in vivo effectiveness. EO and its constituents (thymol, p-cymene, -terpinene and myrcene) displayed cytotoxicity to different tumor cell lines. EO treatment caused G1 arrest in HepG2 cells accompanied by the induction of DNA fragmentation without affecting cell membrane integrity. Cell morphology consistent with apoptosis and a remarkable activation of caspase-3 were also observed, suggesting induction of caspase-dependent apoptotic cell death. In vivo antitumor study showed tumor growth inhibition rates of 38.5–41.9%. In conclusion, the tested essential oil of L. gracilis leaves, which has thymol as its major constituent, possesses significant in vitro and in vivo antitumor activity. These data suggest that leaf essential oil of L. gracilis is a potential medicinal resource

Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill.) N. E. Brown and Biological Activities

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-01-25T13:21:01Z No. of bitstreams: 1 JESUS, R.A. Application of LC-DAD...2018.pdf: 745151 bytes, checksum: b1f9c5870976b802ff921800ba639cf7 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-01-25T13:33:24Z (GMT) No. of bitstreams: 1 JESUS, R.A. Application of LC-DAD...2018.pdf: 745151 bytes, checksum: b1f9c5870976b802ff921800ba639cf7 (MD5)Made available in DSpace on 2019-01-25T13:33:24Z (GMT). No. of bitstreams: 1 JESUS, R.A. Application of LC-DAD...2018.pdf: 745151 bytes, checksum: b1f9c5870976b802ff921800ba639cf7 (MD5) Previous issue date: 2018Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES), Finance Code 001. The authors thank the Brazilian funding agencies CNPq, FAPESB and FAPESP (PROEM 2014/50299-5, research grant 2013/01710-1 and Table 2. Inhibition percentage of the activity of the AChE-ICER Sample AChE-ICER inhibition activity / % Sample AChE-ICER inhibition activity / % LA01IS 6.70 ± 0.8 LA01HS 32.7 ± 3.3 LA02IS 14.6 ± 2.4 LA02HS 27.4 ± 0.7 LA24IS 18.0 ± 1.3 LA24HS 30.3 ± 4.1 LA32IS 13.4 ± 0.9 LA32HS 6.70 ± 0.5 LA39IS 17.1 ± 1.7 LA39HS 18.5 ± 4.0 LA54IS 16.1 ± 1.1 LA54HS 24.5 ± 0.7 LA01IW 10.3 ± 4.7 LA01HW 28.4 ± 0.2 LA02IW 7.60 ± 3.8 LA02HW 27.2 ± 0.3 LA24IW 15.0 ± 1.1 LA24HW 24.4 ± 3.2 LA32IW 11.5 ± 1.1 LA32HW 22.2 ± 0.8 LA39IW 23.3 ± 1.6 LA39HW 23.2 ± 1.6 LA54IW 14.4 ± 0.1 LA54HW 29.3 ± 4.5 Galanthaminea 87.4 ± 2.2 aPositive control of acetylcholinesterase (AchE; 100 μM). AChE-ICER: AChE immobilized enzyme reactor; LA01-LA54: leaf samples of L. alba accessions; IS: infusion summer; HS: hydroalcoholic summer; IW: infusion winter; HW: hydroalcoholic winter. PD grant 2014/11640-3) for their financial support and scholarships. A. F. B., E. R. P. F., D. P. B., C. L. C. and P. C. L. N. are grateful to CNPq for research fellowships.Universidade Federal de Sergipe. Departamento de Química. Cristovão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Química. Cristovão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Engenharia Agronômica. Cristovão, SE, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Sergipe. Departamento de Química. Cristovão, SE, Brasil.Universidade Federal de Sergipe. Departamento de Química. Cristovão, SE, Brasil.Universidade Federal de São Carlos. Departamento de Química. São Carlos-SP, Brasil.Universidade Federal de Sergipe. Departamento de Engenharia Agronômica. Cristovão, SE, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Química. Ribeirão Preto, SP, Brasil.Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Química. Ribeirão Preto, SP, Brasil.Universidade Federal de Sergipe. Departamento de Química. Cristovão, SE, Brasil.The methodology developed in this study through the acquisition of fingerprint chromatograms by liquid chromatography-diode array detector (LC-DAD), aided by principal component analysis, made it possible to assess, in a rational way, the chemical differences between six accessions of Lippia alba and to verify the influence of the method of extraction, the seasonality and the individuality of each accession on chemical composition of its extracts. Among all extracts analyzed against cancer cell lines, eight of them showed to be more promising against a human leukemia cell line (HL-60), displaying cell growth inhibition percentage ranging between 40.0 and 52.0%. In the inhibitory activity assays against acetylcholinesterase enzyme, all extracts showed weak inhibitory effect, with highlight only for six of them, which displayed inhibition ranging between 27.0 and 32.0%

NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics

Xenarthrans—anteaters, sloths, and armadillos—have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records (n = 5,941), and Cyclopes sp. have the fewest (n = 240). The armadillo species with the most data is Dasypus novemcinctus (n = 11,588), and the fewest data are recorded for Calyptophractus retusus (n = 33). With regard to sloth species, Bradypus variegatus has the most records (n = 962), and Bradypus pygmaeus has the fewest (n = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans data set. Please cite this data paper when using its data in publications. We also request that researchers and teachers inform us of how they are using these data