Asaia lannensis bacteremia in a 'needle freak' patient

The genus Asaia has gained much interest lately owing to constant new species discoveries and its role as a potential opportunistic pathogen to humans. Here we describe a transient bacteremia due to Asaia lannensis in a patient with a psychiatric disorder (compulsive self-injection of different substances). Common phenotypic methods of identification failed to identify this organism, and only restriction fragment lenght polymorphism of PCR-amplified 16S rRNA gene allowed for proper identification. The isolate was highly resistant to most antibiotics. The paper also discusses the currently available medical literature, acknowledges the potential problems linked to the isolation of these strains and proposes an approach to species identification that can be applied in a clinical microbiology laboratory

Pokušaji lociranja i uzorkovanja velike bijele psine, Carcharodon carcharias (Lamniformes: Lamnidae), uz talijansku obalu u Sredozemnom moru

Described in the present report are documented attempts to tag and sample the white shark, Carcharodon carcharias (Linnaeus, 1758), along Italian coasts in the Mediterranean Sea, which took place near the Lampedusa Island in the lower Tyrrhenian Channel of Sicily, off the coast of Rimini in the Adriatic Sea, and of Civitavecchia in the central Tyrrhenian Sea. The project, activated in 2015, was aimed at tagging and sampling specimens of the white shark in order to collect data useful to implement conservation strategies in the Mediterranean Sea. Despite four tagging attempts made in 2017, 2018, 2021, and 2022 with 288 total hours of baiting activity and the use of 1030 kg of chum, no white sharks or any other shark species were sighted.U radu su dokumentirani pokušaji označavanja i uzorkovanja velike bijele psine Carcharodon carcharias (Linnaeus, 1758) uz talijansku obalu u Sredozemnom moru. Istraživanje je obavljeno na području oko otoka Lampeduze, u sicilijanskom kanalu u južnom Tirenskom moru, ispred Riminija u Jadranskom moru te kod Civitavecchije u centralnom Tirenskom moru. Projekt, koji je započeo 2015. godine, bio je usmjeren na označavanje i uzorkovanje primjeraka velike bijele psine s ciljem prikupljanja podataka važnih za implementaciju mjera za očuvanje populacije ove vrste u Sredozemnom moru. Unatoč četiri pokušaja označavanja obavljena 2017., 2018., 2021. i 2022. godine, u ukupnom trajanju od 288 sati primamljivanja uz pomoć mamca te 1030 kg mamca, ni jedan primjerak velike bijele psine, ni drugih vrsta morskih pasa, nije primijećen

Full-mouth adhesive rehabilitation in case of severe dental erosion, a minimally invasive approach following the 3-step technique

A full-mouth adhesive rehabilitation in case of severe dental erosion may present a challenge for both the clinician and the laboratory technician, not only for the multiple teeth to be restored, but also for their time schedule, difficult to be included in a busy agenda of a private practice. Thanks to the simplicity of the 3-step technique, full-mouth rehabilitations become easier to handle. In this article the treatment of a very compromised case of dental erosion (ACE class V) is illustrated, implementing only adhesive techniques. The very pleasing clinical outcome was the result of the esthetic, mechanic and most of all biological success achieved, confirming that minimally invasive dentistry should always be the driving motor of any rehabilitation, especially in patients who have already suffered from conspicuous tooth destruction

Evaluation of antiseptic disinfectant activity with static light scattering technology

Background and aims. Healthcare-associated infections (HAI) are an increasingly important issue, for this reason disinfection and antisepsis practices acquire importance. The use of products with antiseptic activity and reports of resistance to these molecules, pose the need to test, in the local area, the sensitivity pattern. The aim of this study is to verify the ability of the analytical system Alfred 60AST (Alifax Spa Isola dell’Abbà, Polverara - PD - Italy) in evaluating the antimicrobial effectiveness in vitro of different molecules in comparison to the membrane filtration reference method, in accordance with the procedure NF T72- 152 proposed by the Association Française de Normalisation (AFNOR). Materials and Methods. We used four antiseptic-disinfectant substances, commonly used in hospital practic: Iodopovidone, Ethanol, Chlorhexidine and DECS. ATCC strains were assessed both with clinical isolates. The eventual development occurs by means of microbial ALFRED AST60 was carried out in progressive times (30, 60 and 120 minutes) with different dilutions for each of the disinfectant molecules tested. Results and Conclusions. Comparison tests carried out between membrane filtration method and instrumentation Alfred 60AST gave results almost totally concordant. The analyzer Alfred 60AST can then be appropriately adapted to the in vitro evaluation of antiseptics, representing a valuable aid in the periodic monitoring of their activities and the prior assessment of sensitivity for therapeutic use. Though preliminary, the study confirms the existence of bacteria resistant to alcohols and biguanides, and it emphasizes the opportunity to verify the in vitro sensitivity profile

Adhesively restored anterior maxillary dentitions affected by severe erosion: up to 6-year results of a prospective clinical study

In case of severe dental erosion, the maxillary anterior teeth are often particularly affected. Restoring such teeth conventionally (ie, crowns) would frequently involve elective endodontic therapy and major additional loss of tooth structure. A novel, minimally invasive approach to restore eroded teeth has been developed and is currently being tested in the form of a prospective clinical trial, termed The Geneva Erosion Study. To avoid crowns, two separate veneers with different paths of insertion have been used to restore the affected anterior maxillary teeth, regardless of clinical crown length and amount of remaining enamel. This treatment is called The Sandwich Approac

Biological outcomes and patient-reported outcome measures (PROMs) of minimally invasive full-mouth rehabilitations of patients with erosions and/or abrasions by means of the "3-step technique"...

To evaluate biologic and esthetic outcomes, as well as the patient-reported outcome measures (PROMs), of full-mouth rehabilitations in patients suffering from generalized erosive and/or abrasive tooth wear following the 3-step technique

Retrospective clinical study of minimally invasive full-mouth rehabilitations of patients with erosions and/or abrasions following the "3-step technique". part 1: 6-year survival rates and technical outcomes of the restorations

To evaluate the survival rates and technical outcomes of minimally invasive full-mouth rehabilitations in patients affected by dental erosion and attrition