Medieval Nemea: Building a Public Digital Resource

This site presents medieval ceramics from the excavations at the site of Nemea, in southern Greece. We have created a digital resource with results and artifacts from archaeological excavations conducted in the 1970s and 1980s, which have remained unpublished. The website incorporates a variety of materials, including excavation notebooks, maps, data bases, photographs, and 3D models of ceramics. Omeka was selected as the software for this project for several reasons. Omeka offers the Dublin Core metadata as a way to standardize and organize digital data, allowing its users access to a well-developed platform. Omeka is also an open source software allowing anyone to build and access digital resources, as well as the opportunity to share cultural heritage resources worldwide. Lastly, plugins are useful tools which allow users to broaden the fundamental functionality of Omeka. As digital technologies progress, so will our abilities to enhance this digital resource. Although Omeka was created for a 2D environment, we anticipate that soon it will offer tools that will allow the incorporation of 3D data. In the near future, we plan to create an open forum and invite comments and suggestions from users for improvements of this digital resource. Another addition will be to create a ‘How-to E-book’ page that will explain the process and the steps that we have followed, and make the addition of new content easy and user-friendly. Finally, resources such as this strengthen the connection between research, heritage and preservation, by broadening participation and making accessible material that previously had been available only to specialists

The neuronal guidance protein netrin-1 reduces alveolar inflammation in a porcine model of acute lung injury

Introduction: Acute lung injury (ALI) is an inflammatory disorder of pulmonary or extrapulmonary origin. We have previously demonstrated that netrin-1 dampens murine ALI, and in an attempt to advance this finding into future clinical practice we evaluated whether netrin-1 would reduce alveolar inflammation during porcine ALI. Methods: This was a controlled in vivo experimental study in pigs. We induced ALI through lipoploysaccharide (LPS) infusion (50 micro g/kg) for 2 hours. Following this, we exposed animals to either vehicle, intravenous netrin-1 (netrin-1 i.v.) or inhaled netrin-1 (netrin-1 inh.). Serum samples and bronchoalveolar lavage (BAL) were obtained to determine levels of tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, interleukin-6 and interleukin-8 at baseline and 6 hours following treatment. Myeloperoxidase activity (MPO) and protein levels were determined in the BAL, and tissue samples were obtained for histological evaluation. Finally, animals were scanned with spiral CT. Results: Following LPS infusion, animals developed acute pulmonary injury. Serum levels of TNF-alpha and IL-6 were significantly reduced in the netrin-1 i.v. group. BAL demonstrated significantly reduced cytokine levels 6 hours post-netrin-1 treatment (TNF-alpha: vehicle 633 ± 172 pg/ml, netrin-1 i.v. 84 ± 5 pg/ml, netrin-1 inh. 168 ± 74 pg/ml; both P < 0.05). MPO activity and protein content were significantly reduced in BAL samples from netrin-1-treated animals. Histological sections confirmed reduced inflammatory changes in the netrin-1-treated animals. Computed tomography corroborated reduced pulmonary damage in both netrin-1-treated groups. Conclusions: We conclude that treatment with the endogenous anti-inflammatory protein netrin-1 reduces pulmonary inflammation during the initial stages of ALI and should be pursued as a future therapeutic option