Types of reservoirs of the Ufa stage of the Republic of Tatarstan. Genesis and reservoir properties

In this paper, we studied core material from well No. 15 of the extra-viscous oil field, geographically located within the western border of the South Tatar arch. Under laboratory conditions, reservoir properties (porosity, permeability), oil saturation and particle size distribution of Sheshmin sandstones were measured. It was established that the terrigenous reservoir belongs to class I and II according to the classification of A.A. Hanin, with high permeability. In addition, in well No. 15 , a downward trend was identified in reservoir properties downstream of the section, the reason for which is probably the migration of underlying formation waters from carbonate sediments of Sakmar age. By analyzing the grain size data distribution, the reservoir is represented by well-sorted fine-grained sandstone with a dominant fraction of 0.1-0.25 mm (about 65% of the entire sample); paleodynamic analysis was carried out using the Passega diagram; It was established that the formation of the reservoir took place under conditions of gradation suspension (P-Q-R area in the diagram), in the lower parts of fast river flows, directly at the bottom. The findings are consistent with data from previous researchers. According to the study of the cores of other wells, maps of changes in reservoir properties have also been constructed, which highlighted reservoir zones with high reservoir properties – the central parts of the North and South Uplifts

TECHNOLOGICAL PROPERTIES OF MILK OF COWS WITH DIFFERENT GENOTYPES OF KAPPA-CASEIN AND BETA-LACTOGLOBULIN

The presence of the desirable alleles and genotypes of casein and whey protein genes in the genome of cows affects the milk protein content, quality and technological properties of their milk. Two important properties of milk its producibility is judged on are cheeseability and heat resistance. The present studies aimed at estimating the technological properties of milk of black-motley × Holstein and Kholmogorskaya breeds cows of the Tatarstan type with different kappa-casein (CSN3) and beta-lactoglobulin (BLG) genotypes. The study was carried out using a sampling of the first-calf cows of 5 cattle-breeding farms of the Republic of Tatarstan. In animals, the CSN3 and BLG genotypes have been determined by a PCR-RFLP analysis. The cheeseability, heat resistance and thermostability of milk have been estimated using standard methods. The studies have established that the CSN3 and BLG genotypes of cows affected the condition of a casein clot and duration of milk clotting time. The best cheese-making properties of milk were inherent in the animals with the BB and AB genotypes of the CSN3 and BLG genes. They were superior to the coevals with the AA genotype in terms of the highest yield of the desired dense casein clot and the shortest duration of milk clotting time. The first-calf cows, which are the carriers of an A allele of the CSN3 gene, were superior to the animals with the BB genotype of the CSN3 gene on the thermostability of milk including that on the proportion of animals with this milk characteristic. The BLG genotype of the studied animals did not significantly affect the thermostability of milk. Moreover, the highest thermostability of milk was characteristic of black-motley × Holstein cows with the AA genotype

Phylotyping and genotyping of Escherichia coli isolates obtained from patients with colorectal cancer

© 2020, Advanced Scientific Research. All rights reserved. Objective of the research was to phylotype the E. coli isolates from the malignant and adjacent normal epithelium of the rectal mucosa of patients diagnosed with colorectal cancer, identifying and discriminating phylogroups by a set of genes, and also genotyping them by the fimH-gene locus by identifying fimH types. Samples of bacterial cultures isolated from paired biopsy specimens of patients diagnosed with colorectal cancer were subjected to molecular genetic research for species identification, as well as E. coli phylo- and genotyping. By sequencing the 16S rRNA locus, bacterial cultures are identified both as isolates of monocultures of E. coli and as mixed cultures of E. coli and K. pneumonia. The procedure for the phylotyping of E. coli isolates by identifying and discriminating phylogroups by a set of genes established the affiliation of samples of bacterial cultures with phylogroups A and B2. The genotyping procedure for E. coli isolates at the fimH gene locus by identifying the fimH types characterized the studied samples by signs of the third (f-3), fourth (f-4), and seventh (f-7) types, respectively. The strategy for identifying fimH-types during genotyping of E. coli at the fimH-gene locus has been improved, based on the analysis of SNP sequenced DNA sequences, and includes 52 fimH-types, three of which are justified in this paper