9 research outputs found
Insecticidal and genotoxic activity of Psoralea corylifolia Linn. (Fabaceae) against Culex quinquefasciatus Say, 1823
Gas chromatography mass spectrometry analysis and larvicidal activity of leaf essential oil extract of Leucas aspera against dengue, malaria and filariasis vectors
Water extracts of Brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L.
This study assessed the toxicity of seed water extracts of 15 leguminous species upon Aedes aegypti larvae. A partial chemical and biochemical characterization of water extracts, as well as the assessment of their acute toxicity in mice, were performed. The extracts of Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum and Piptadenia moniliformis caused 100% of mortalit y after 1 to 3 h of exposure. They showed LC50 and LC90 values ranging from 0.43 ± 0.01 to 9.06 ± 0.12 mg/mL and from 0.71 ± 0.02 to 13.03 ± 0.15 mg/mL, respectively. Among the secondary metabolite constituents, the seed water extracts showed tannins, phenols, flavones, favonols, xanthones, saponins and alkaloids. The extracts also showed high soluble proteins content (0.98 to 7.71 mg/mL), lectin (32 to 256 HU/mL) and trypsin inhibitory activity (3.64 = 0.43 to 26.19 = 0.05 gIT/kg of flour) The electrophoretic profiles showed a great diversity of protein bands, many of which already described as insecticide proteins. The extracts showed low toxicity to mice (LD50 > 0.15 = 0.01 g/kg body weight), but despite these promising results, further studies are necessary to understand the toxicity of these extracts and their constituentsfrom primary and secondary metabolism upon Ae. aegypti.<br>Este trabalho objetivou avaliar a toxicidade dos extratos aquosos de sementes de 15 espécies de leguminosas contra larvas de Aedes aegypti. Foi realizada uma caracterização química e bioquímica parcial dos extratos aquosos e a avaliação da toxicidade aguda em camundongos. Os extratos de Amburana cearensis, Anadenanthera macrocarpa, Dioclea megacarpa, Enterolobium contortisiliquum e Piptadenia moniliformis causaram 100% de mortalidade depois de 1 a 3 h de exposição e mostraram valores de CL50 e CL90 entre 0,43 = 0,01 e 9,06 ± 0,12 e entre 0,71 = 0,02 e 13,03 = 0,15 mg/mL, respectivamente. Dentre os constituintes do metabolismo secundário, os extratos das sementes apresentaram taninos, fenóis, flavonas, flavonóis, xantonas, saponinas e alcalóides. Os extratos apresentaram alto teor de proteínas solúveis (0,98 to 7,71 mg/mL), lectina (32 to 256 UH/mL) e inibidor de tripsina (3,64 ± 0,43 to 26,19 = 0,05 gIT/kg de farinha). O perfil eletroforéticomostrou uma grande diversidade de proteínas, muitas dasquais já descritas como inseticidas. Os extratos mostraram baixa toxicidade ao camundongo (DL50 > 0,15 = 0,01 g/kg peso corporal), porém apesar desses resultados promissores, estudos posteriores são necessários para compreender a toxicidade desses extratos e de seus constituintes do metabolismo primário e secundário sobre Ae. aegypti
Eugenol, α-pinene and β-caryophyllene from Plectranthus barbatus essential oil as eco-friendly larvicides against malaria, dengue and Japanese encephalitis mosquito vectors
Mosquito-borne diseases represent a deadly threat for millions of people worldwide. Eco-friendly mosquitocides are a priority. In Ayurvedic medicine, Plectranthus species have been used to treat heart disease, convulsions, spasmodic pain and painful urination. In this research, we evaluated the acute toxicity of essential oil from Plectranthus barbatus and its major constituents, against larvae of the malaria vector Anopheles subpictus, the dengue vector Aedes albopictus and the Japanese encephalitis vector Culex tritaeniorhynchus. The chemical composition of P. barbatus essential oil was analyzed by gas chromatography–mass spectroscopy. Nineteen components were identified. Major constituents were eugenol (31.12 %), α-pinene (19.38 %) and β-caryophyllene (18.42 %). Acute toxicity against early third-instar larvae of An. subpictus, Ae. albopictus and Cx. tritaeniorhynchus was investigated. The essential oil had a significant toxic effect against larvae of An. subpictus, Ae. albopictus and Cx. tritaeniorhynchus, with 50 % lethal concentration (LC50) values of 84.20, 87.25 and 94.34 μg/ml and 90 % lethal concentration (LC90) values of 165.25, 170.56 and 179.58 μg/ml, respectively. Concerning major constituents, eugenol, α-pinene and β-caryophyllene appeared to be most effective against An. subpictus (LC50 = 25.45, 32.09 and 41.66 μg/ml, respectively), followed by Ae. albopictus (LC50 = 28.14, 34.09 and 44.77 μg/ml, respectively) and Cx. tritaeniorhynchus (LC50 = 30.80, 36.75 and 48.17 μg/ml, respectively). Overall, the chance to use metabolites from P. barbatus essential oil against mosquito vectors seems promising, since they are effective at low doses and could be an advantageous alternative to build newer and safer mosquito control tools
Eugenol, α-pinene and β-caryophyllene from Plectranthus barbatus essential oil as eco-friendly larvicides against malaria, dengue and Japanese encephalitis mosquito vectors
High efficacy of (Z)-γ-Bisabolene from the essential oil of Galinsoga parviflora (Asteraceae) as larvicide and oviposition deterrent against six mosquito vectors
The eco-friendly control of mosquitoes with novel and effective larvicides and oviposition deterrents is a crucial challenge to prevent outbreaks of mosquito-borne diseases. However, most of the herbal formulations tested in these years showed LC50 values higher of 10 ppm, and oviposition deterrent activity only when tested at relatively higher doses (>50 µg/ml). Here, we focused on the chemical composition of the essential oil (EO) of Galinsoga parviflora, an annual herb native to South America and naturalized all over the world, testing its larvicidal and oviposition deterrent action against 6 mosquito species. Totally 37 compounds were identified in the EO of G. parviflora by GC and GC-MS analyses. The major constituent was (Z)-γ-bisabolene (38.9%). The G. parviflora EO and (Z)-γ-bisabolene showed acute toxicity on An. stephensi (LC50=31.04 and 2.04 µg/ml), Ae. aegypti (LC50=34.22 and 2.26 µg/ml), Cx. quinquefasciatus (LC50=37.10 and 2.47 µg/ml), An. subpictus (LC50=40.97 and 4.09µg/ml), Ae. albopictus (LC50=45.55 and 4.50µg/ml) and Cx. tritaeniorhynchus (LC50=49.56 and 4.87 µg/ml) larvae. Furthermore, the oviposition deterrent potential of the G. parviflora EO and (Z)-γ-bisabolene was studied on six mosquito vectors, showing that 25 µg/ml of (Z)-γ-bisabolene led to Oviposition Activity Index lower of -0.79 in all tested mosquito vectors. Overall, all larvicidal LC50 values estimated for (Z)-γ-bisabolene were lower than 5 µg/ml, this result far encompass current evidences of toxicity reported for the large majority of botanical products currently tested against mosquito young instars, allowing us to propose this compound as mosquito larvicide and oviposition deterrent
High efficacy of (Z)-γ-bisabolene from the essential oil of Galinsoga parviflora (Asteraceae) as larvicide and oviposition deterrent against six mosquito vectors
Essential Oils from Aromatic and Medicinal Plants as Effective Weapons Against Mosquito Vectors of Public Health Importance
The fight against mosquito-borne diseases has recently seen the failure of control programmes based on synthetic chemical treatments to combat larvae and adults of mosquito vectors. This has led to several problems linked to residual substances causing a detrimental impact on environment and human health and to the development of resistance in mosquitoes. In this scenario, new eco-friendly and alternative strategies for the management of mosquito-borne diseases come from the use of plant essential oils (EOs). These are complex mixtures of small, volatile and lipophilic compounds, mostly belonging to monoterpenoids, sesquiterpenoids and phenylpropanoids, produced by aromatic plants belonging to several botanical families such as Apiaceae, Asteraceae, Geraniaceae, Lamiaceae, Lauraceae, Myrtaceae, Poaceae, Rutaceae, Verbenaceae and Zingiberaceae. An important ecological role played by EOs is defending plants from several enemies such as bacterial and fungal pathogens, viruses, insects and parasites. EOs represent ideal candidate ingredients to be incorporated in insecticidal formulations since scientific evidences have documented their efficacy against larvae and adults of several mosquitoes (e.g. Anopheles, Aedes and Culex) even at low doses (<50 ppm), the multiple mode of action and wide spectrum of efficacy, the low toxicity on nontarget organisms and environment and the unlikely capacity to induce insect resistance. In this chapter, we gave an overview of the most important EOs obtained from commercially important botanical families with documented efficacy against mosquito vectors. Particular attention has been paid to highlight their strengths and weakness and the future challenges leading to the replacement of conventional insecticides by agrochemical companies