Reação de linhagens de feijoeiro de oito ciclos de seleção recorrente visando a resistência à Pseudocercospora griseola a dois isolados do patógeno.

A mancha angular incitada pelo fungo Pseudocercospora griseola é um dos principais problemas da cultura do feijoeiro em algumas regiões do Brasil. Visando a obter linhagens resistentes a esse patógeno, um programa de seleção recorrente vem sendo conduzido há alguns anos pela Universidade Federal de Lavras (UFLA) e Embrapa Arroz e Feijão. Esse trabalho visa avaliar o desempenho de linhagens obtidas nos diferentes ciclos seletivos, sob condições de casa de vegetação, com relação a dois isolados de P griseola

Risk Factors for Recurrent Wheezing in Infants

AbstractIntroduction: wheezing is one of the most common respiratory symptoms in childhood. Regardless of the cause, it is a reason to seek medical care in emergency rooms, especially if there is recurrence of episodes. Very common in childhood, recurrent wheezing has its first episodes in the first year of life. We sought to examine the risk factors for recurrent wheezing in infants in the first year of life. Methods: this is a cross- sectional quantitative study in which a standardized questionnaire of the International Study of Wheezing in Infants, translated and validated in Brazil, consisting of objective questions, applied 40 mothers were enrolled in two Family Health units. Results: the risk factors found were: smoking during pregnancy, family history of asthma, rhinitis and allergic dermatitis, the presence of at least one pet in the home at the time of birth and age at first cold less than or equal to three months of life. No significant relationships were found between males and wheezing, exclusive breastfeeding or numbers of colds in the first year of life. Conclusion: our findings are different from those reported in the literature.ResumoIntrodução: a sibilância é um dos sintomas respiratórios mais comuns na infância. Independentemente da causa, é motivo de procura por atendimento médico em serviços de urgência, sobretudo se há recorrência dos episódios. Muito frequente na infância, a sibilância de repetição tem seus primeiros episódios no primeiro ano de vida. Objetivo: verificar os fatores de risco para sibilância recorrente em lactentes no primeiro ano de vida. Método: pesquisa de campo do tipo exploratória, transversal com abordagem quantitativa, onde foi aplicado um questionário padronizado do Estudo Internacional de Sibilância em Lactentes, traduzido e validado no Brasil, constituído por questões objetivas. Foram questionadas 40 mães cadastradas em duas Unidades de Saúde da Família. Resultados: os fatores de risco encontrados: tabagismo durante a gestação, histórico familiar de asma, rinite e dermatite alérgica, presença de pelo menos um animal doméstico em domicílio na época do nascimento e idade do primeiro resfriado menor ou igual a três meses de vida. Não foram encontradas relações significativas entre sibilância e gênero masculino, amamentação exclusiva e quantidade de resfriados no primeiro ano de vida. Conclusão: mesmo utilizando um instrumento de pesquisa padronizado, é notória a discrepância nos resultados encontrados

Marker-assisted elimination of drought-susceptible accessions in upland rice breeding.

Breeding for water-deficit tolerance is fundamental to guarantee the sustainability of upland rice production, mainly due to the possibility of an increased frequency of drought episodes due to climate change. This work aimed to identify single nucleotide polymorphism (SNP) markers, derived from RNA sequencing (RNA-Seq), genome-wide association study (GWAS) and candidate genes from Arabidopsis, with potential for use in marker-assisted selection (MAS) for drought tolerance. RNA-Seq and GWAS were efficient in identifying useful SNP markers from the data obtained from three years of field experiments for 175 upland rice accessions, which were sequenced using 32 genes by Capture-Seq. Three genes were equally able to generate SNP markers that discriminated 95% of the 20 most drought susceptible accessions in the joint analysis of the experiments. The elimination of the genotypes with the unfavourable SNP allele reduced the initial number of accessions to one third, and transferring this result in a breeding routine, would enable to conduct smaller experiments per target location, increasing the precision and reducing the cost of the drought phenotyping

Evaluation of the maize (Zea mays L.) diversity on the Archipelago of Madeira

The variability of 43 open-pollinated populations of maize (Zea mays L.), representing a wide range of ecological conditions on the Archipelago of Madeira, was evaluated based on the morphological and reproductive traits. Individual data of 41 traits related to earliness, plant and tassel structure and the shape of the ear and grain were analysed using multivariate analysis. The populations belonging to two major maize varieties were grouped into four groups by their degree of dissimilarity, based on discriminant analysis. The dissimilarity of these groups was confirmed by the values of the Tukey test. The racial rank of these groups was proposed and a brief description of the maize landraces was presented. This work represents the first morphological characterization and analysis of diversity of maize germplasm for the Archipelago of Madeira where the traditional agricultural practices are still keeping this Portuguese region free from corn hybrids. The description of the Madeiran corn landraces allows us to preserve the existing corn biodiversity and could be used for their registration as conservation landraces or for conservation and breeding proposes worldwide.Portuguese Foundation for the Science and Technology (FCT, Fundac¸a˜o para a Cieˆncia e Tecnologia) has sponsored this work, through the Centre of Macaronesian Studies (CEM) and the project POCTI no35003/AGR/2001. The Authors are grateful to the Madeiran farmers who assisted with collection of maize samples.info:eu-repo/semantics/publishedVersio

AUTOMATIC METHOD FOR GLAUCOMA CLASSIFICATION USING TEXTURE ANALYSIS, XGBOOST AND GRID SEARCH

Glaucoma is an irreversible pathology, generated by increased intraocular pressure. Early detection is critical and can pre- vent total vision loss. Clinical examinations are commonly used to detect the disease. Still, the time and cost of identi- fication is quite high. This paper presents a computational methodology that aims to assist specialists in the discov- ery of glaucoma through Computer Vision techniques. The proposed methodology consists in the application of several texture descriptors combined with a parameter optimiza- tion done through Grid search with the XGBoost classifier. A result was obtained with accuracy of 82.37% and ROC of 82.08%

Valor de cultivo e uso de linhagens de feijoeiro de grãos tipo carioca em Minas Gerais no período de 2010 a 2012.

Nesse trabalho são apresentados os resultados dos ensaios de VCU de feijão tipo carioca conduzidos em Minas Gerais pela UFLA, UFV, Embrapa e Epamig nos anos de 2010 a 2012, visando a indicação de nova cultivar com esse tipo de grão para o estado. Foram avaliadas 20 novas linhagens de feijoeiro juntamente com as testemunhas Pérola, BRSMG Talismã, BRSMG Majestoso e BRSMG Madrepérola, já registradas para o estado de Minas Gerais, e a cultivar BRS Notável, registrada para cultivo em outros estados

Valor de cultivo e uso de linhagens de feijoeiro de grãos tipo carioca em Minas Gerais no período de 2010 a 2012.

Nesse trabalho são apresentados os resultados dos ensaios de VCU de feijão tipo carioca conduzidos em Minas Gerais pela UFLA, UFV, Embrapa e Epamig nos anos de 2010 a 2012, visando a indicação de nova cultivar com esse tipo de grão para o estado. Foram avaliadas 20 novas linhagens de feijoeiro juntamente com as testemunhas Pérola, BRSMG Talismã, BRSMG Majestoso e BRSMG Madrepérola, já registradas para o estado de Minas Gerais, e a cultivar BRS Notável, registrada para cultivo em outros estados

A novel leptospiral protein increases ICAM-1 and E-selectin expression in human umbilical vein endothelial cells

It has been reported previously that activation of vascular endothelium by outer membrane proteins of the spirochetes Borrelia sp. and Treponema sp. resulted in enhanced expression of endothelial cell adhesion molecules. To investigate the role of leptospiral proteins in this process, a predicted lipoprotein encoded by the gene LIC10365 was selected, which belongs to a paralogous family that presents a domain of unknown function, DUF1565. The LIC10365 gene was cloned and the protein expressed in Escherichia coli C43 (DE3) strain using the vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and was used to assess its ability to activate cultured human umbilical vein endothelial cells. The rLIC10365 activated endothelium in such a manner that E-selectin and intercellular adhesion molecule 1 (ICAM-1) became upregulated in a dose-dependent fashion. The LIC10365-encoded protein was identified in vivo in the renal tubules of animal during experimental infection with Leptospira interrogans. Collectively, these results implicate the LIC10365-coding protein of L. interrogans as a potential effector molecule in the promotion of a host inflammatory response. This is the first report of a leptospiral protein capable of up-regulating the expression of endothelial cell adhesion molecules ICAM-1 and E-selectin.Instituto de Biotecnologia y Biologia Molecula

Estabilidade de linhagens de feijoeiro comum no ensaio intermediário da Embrapa, ciclo 2007.

O objetivo deste trabalho foi avaliar a estabilidade e a adaptabilidade das linhagens do feijoeiro comum do grupo comercial carioca no Ensaio Intermediário da Embrapa, visando futura indicação de novas linhagens para diferentes regiões produtoras