Determinants of Web 2.0 technologies for knowledge sharing in SMEs.

ABSTRACT: Web 2.0 technologies are increasingly being used within all knowledge management processes. This paper extends previous studies on the use of internet technologies and knowledge management by analyzing factors affecting knowledge sharing through Web 2.0 technologies within small and medium-sized enterprises (SMEs). Drawing upon the technology-organization-environment framework, a model is developed to examine how distinct contextual factors influence the use of Web 2.0 technologies for knowledge sharing. A data set of Spanish SMEs is used to test the conceptual model and hypotheses. Results show that knowledge sharing through Web 2.0 technologies emerges from internal organizational and technological resources rather than from external pressure

Click-EM for imaging metabolically tagged nonprotein biomolecules

EM has long been the main technique for imaging cell structures with nanometer resolution but has lagged behind light microscopy in the crucial ability to make specific molecules stand out. Here we introduce click-EM, a labeling technique for correlative light microscopy and EM imaging of nonprotein biomolecules. In this approach, metabolic labeling substrates containing bioorthogonal functional groups are provided to cells for incorporation into biopolymers by endogenous biosynthetic machinery. The unique chemical functionality of these analogs is exploited for selective attachment of singlet oxygen-generating fluorescent dyes via bioorthogonal 'click chemistry' ligations. Illumination of dye-labeled structures generates singlet oxygen to locally catalyze the polymerization of diaminobenzidine into an osmiophilic reaction product that is readily imaged by EM. We describe the application of click-EM in imaging metabolically tagged DNA, RNA and lipids in cultured cells and neurons and highlight its use in tracking peptidoglycan synthesis in the Gram-positive bacterium Listeria monocytogenes