13 research outputs found
Front Immunol
HIV-2 infection is characterized by low viremia and slow disease progression as compared to HIV-1 infection. Circulating CD14++CD16+ monocytes were found to accumulate and CD11c+ conventional dendritic cells (cDC) to be depleted in a Portuguese cohort of people living with HIV-2 (PLWHIV-2), compared to blood bank healthy donors (HD). We studied more precisely classical monocytes; CD16+ inflammatory (intermediate, non-classical and slan+ monocytes, known to accumulate during viremic HIV-1 infection); cDC1, important for cross-presentation, and cDC2, both depleted during HIV-1 infection. We analyzed by flow cytometry these PBMC subsets from Paris area residents: 29 asymptomatic, untreated PLWHIV-2 from the IMMUNOVIR-2 study, part of the ANRS-CO5 HIV-2 cohort: 19 long-term non-progressors (LTNP; infection â„8 years, undetectable viral load, stable CD4 countsâ„500/ÎŒL; 17 of West-African origin -WA), and 10 non-LTNP (P; progressive infection; 9 WA); and 30 age-and sex-matched controls: 16 blood bank HD with unknown geographical origin, and 10 HD of WA origin (GeoHD). We measured plasma bacterial translocation markers by ELISA. Non-classical monocyte counts were higher in GeoHD than in HD (54 vs. 32 cells/ÎŒL, p = 0.0002). Slan+ monocyte counts were twice as high in GeoHD than in HD (WA: 28 vs. 13 cells/ÎŒL, p = 0.0002). Thus cell counts were compared only between participants of WA origin. They were similar in LTNP, P and GeoHD, indicating that there were no HIV-2 related differences. cDC counts did not show major differences between the groups. Interestingly, inflammatory monocyte counts correlated with plasma sCD14 and LBP only in PLWHIV-2, especially LTNP, and not in GeoHD. In conclusion, in LTNP PLWHIV-2, inflammatory monocyte counts correlated with LBP or sCD14 plasma levels, indicating a potential innate immune response to subclinical bacterial translocation. As GeoHD had higher inflammatory monocyte counts than HD, our data also show that specific controls are important to refine innate immunity studies
High-performance liquid chromatographic investigation of the enantioselectivity and mechanism of chiral recognition of new cholic acid-based stationary phases
International audienc
Mechanistic investigation of enantiomeric separation on new chiral stationary phases prepared from cholic acid and deoxycholic acid derivatives
International audienc
Synthesis, characterization and enantioselectivity of cholic acid-bonded phases for high performance liquid chromatography
International audienc
COMPARISON OF GRAFTING MODES FOR THE PREPARATION OF CHOLIC ACID-BASED STATIONARY PHASES. INFLUENCE ON ENANTIOMER SEPARATIONS IN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
International audienc
Preliminary results on the binary system : (3-phenylcarbamate) methyl-cholanoate ester with toluene
International audienc