Conversion of red fluorescent protein into a bright blue probe

We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. The strategy was applied to red fluorescent proteins of the different genetic backgrounds, such as TagRFP, mCherry, HcRed1, M355NA, and mKeima, which all were converted into blue probes. Further improvement of the blue variant of TagRFP by random mutagenesis resulted in an enhanced monomeric protein, mTagBFP, characterized by the substantially higher brightness, the faster chromophore maturation, and the higher pH stability than blue fluorescent proteins with a histidine in the chromophore. The detailed biochemical and photochemical analysis indicates that mTagBFP is the true monomeric protein tag for multicolor and lifetime imaging, as well as the outstanding donor for green fluorescent proteins in Forster resonance energy transfer applications

Voskresensky New Jerusalem Monastery: Place of Prince A.A. Chelakaev (Chelokaev) in the Family Necropolis of the Buturlins – Priklonskys – Volynskys – Wolfs – Sukhovo-Kobylins

Our research of necropolis of New Jerusalem Monastery has brought a new name. It is prince A.A. Chelakaev. He was buried on the unpaved section of the monastery cemetery. Historic burial place of one of the oldest princely family of Georgia in a noble part of monastery necropolis let us attribute prince A.A. Chelakaev to related families – the Buturlins – Priklonskys – Volynskis – Wolfs – Sukhovo-Kobylins (25/10/1778 – 04/11/1873), including 11 graves. Besides, that extends investigations based on the “monument / burial” principle. Genealogical research that affected the person buried in the monastery of Prince Alexander Anofrievich Chelakaev allowed to make up for the genealogy of Chelokashvili princes, one of the most noble families of Georgia so far very poorly studied. We used the sources associated with burials in Moscow the Georgian elite tombs of St. Nicholas Greek and the Donskoy Monastery. Georgian prince, buried in the Voskresensky New Jerusalem Monastery, was not an unauthorized person to a host of representatives of persons belonging to the aristocracy reigning House of Bagration. We note that the research material at our disposal expanded the results of archaeological discoveries of 2009-2013. Due to this facxt, we updated the list of monuments involving research field associated with the study of the necropolis of the New Jerusalem Monastery in the genealogical, heraldic and epigraphic monuments from 1656 to 1919, as we attributed several previously unknown and inaccessible tombstones before hidden in the ground. In particular, the findings of two new graves and tombstones proved to be especially valuable