Effects of salt stress on volatile compounds, total phenolic content and antioxidant activities of Salvia mirzayanii

AbstractSalvia mirzayanii is a medicinal and aromatic plant belonging to the Lamiaceae family, which is an endemic plant in Iran. In this study, the effects of different salt concentrations on total phenolic content, antioxidant activities and volatile components of the aerial parts of S. mirzayanii were studied. The results showed that total phenolic content increased with the increase in salt concentration. The increase was more pronounced under moderate salinity (3.8mg GAEg−1 DW at 6.8dSm−1 NaCl). Plants grown at 6.8dSm−1 NaCl displayed the highest DPPH˚ scavenging activity with the lowest IC50 value (2.13mgml−1) compared to the control. The volatile components were identified and analyzed by HS (headspace)-GC–MS using the Combi PAL System technique. The main components of control plants were α-terpinyl acetate, 1,8-cineole and bicyclogermacrene. The proportions of these main compounds were differently affected by salinity stress. The results showed that the synthesis of both total phenolic and some important volatile components was induced by moderate salinity

The role of peptides in bone healing and regeneration: A systematic review

Background: Bone tissue engineering and the research surrounding peptides has expanded significantly over the last few decades. Several peptides have been shown to support and stimulate the bone healing response and have been proposed as therapeutic vehicles for clinical use. The aim of this comprehensive review is to present the clinical and experimental studies analysing the potential role of peptides for bone healing and bone regeneration. Methods: A systematic review according to PRISMA guidelines was conducted. Articles presenting peptides capable of exerting an upregulatory effect on osteoprogenitor cells and bone healing were included in the study. Results: Based on the available literature, a significant amount of experimental in vitro and in vivo evidence exists. Several peptides were found to upregulate the bone healing response in experimental models and could act as potential candidates for future clinical applications. However, from the available peptides that reached the level of clinical trials, the presented results are limited. Conclusion: Further research is desirable to shed more light into the processes governing the osteoprogenitor cellular responses. With further advances in the field of biomimetic materials and scaffolds, new treatment modalities for bone repair will emerge

Skład chemiczny olejku eterycznego Scaligeria meifolia Boiss. rosnącego w Iranie

Scaligeria meifolia Boiss., belonging to the Apiaceae family, grows wild in Iran. The essential oil from aerial parts of S. meifolia were obtained by hydrodistillation and analyzed by GC and GC/MS. Thirty-one constituents representing 99.98% of total oil components were identified. The main constituents of the essential oil were germacrene–D, (24.2%), germacrene-B (14.8%), limonene (14.2%), γ-elemene (11.6 %) and β-elemene (5.2%).Scaligeria meifolia Boiss. należąca do rodziny Apiaceae, rośnie dziko w Iranie. Olejek eteryczny z nadziemnych części S. meifolia uzyskano i analizowano za pomocą GC i GC/MS. Zidentyfikowano 31 składników, co stanowi 99,98% wszystkich składników olejku. Najważniejszymi składnikami olejku eterycznego były: germakren-D (24,2%), germakren- B (14,8%), limonen (14,2%), γ-elemen (11,6%) i β-elemen (5,2%)

An Investigation into the Effect of Gamma Irradiation on Microbial Load of Medicinal Plant of Peppermint, Iranian Thyme, Summer Savory and Lemon Balm

Gamma irradiation can be used to increase post harvest quality of medicinal plants by decreasing microbial load and decontamination. Microbiological changes associated with food irradiation include the reduction or elimination of spoilage and pathogenic microorganisms, insect disinfestation and parasite disinfection. This factorial experiment was conducted on the basis of randomized complete block design with three replications. The effects of gamma irradiation on decreasing the microbial load of some medicinal plants including (Mentha piperita), lemon balm (Melissa officinalis), Summer savory (Satureja hortensis) and (Zataria multiflora) were investigated. Leaves of Lemon Balm, summer savory, pepper minit and thyme were irradiated at dosages of 3, 7, 10 and 15 KGy by a Co60 source, then total count, total Coliform and mold and yeast of the samples were studied. Gamma irradiation decreased microbial load of medicinal plant samples. The microbial load was detected in samples treated with 15 KGy radiation and control. Also, the result showed the highest and the lowest microbial load in peppermint and Iranian thyme, respectively. Results of this study show that gamma irradiation can be used as an important decontamination method to reduce the microbial load of medicinal plants

Evaluation of the Effect of Storage Temperature, Atmosphere and Packaging Materials on Some Properties of Carum copticum Fruits during Nine Months Storage

Introduction: The aim of medicinal plant storage is to preserve qualitative and quantitative properties of active substance. Carum copticum fruits (Zenyan in Persian) were used for its therapeutic effects. Seed storage condition after harvest till to extraction time is not suitable in our country and the major part of seed quality deteriorates during the storage period. So, the loss of seed qualitative and quantitative characteristics will increase during unsuitable storage condition. Appropriate storage conditions and management preserve seed active substance, seed viability and vigor for long periods by reducing the rate of seed deterioration. Optimal seed storage can be achieved by modifying the environment around the seeds. Numerous storage systems have evolved over the years for post harvest preservation of crop seeds. The aim of this study is to evaluate the effect of various storage conditions and storage time on essential oil percentage and germination factors in C. copticum seeds during the storage period. The results of this research will be used for optimum storage of these seeds to better preserve their quality. Materials and Methods: In order to investigate the effects of storage conditions (packaging materials and temperature) and storage time on quality of C. copticum stored seeds, a split-plot factorial arranged in a randomized complete block design with three replications is performed in Faculty of Agriculture at Ferdowsi University of Mashhad during 2013 and 2014.Tukey's range test was performed to determine the significant difference between treatments. The calculations were conducted by JMP 8 software. Temperature) at two levels: 20±3°C and 30±3°C( as main plots and packaging materials (at six levels: paper, polyethylene, aluminum foil under vacuum condition, Polyethylene-polyamide packages under vacuum condition, Polyethylene-polyamide packages with a gas composition of [98%N2 + 2%O2] and [90%N2 +10%O2] ) and storage periods (at four levels: 0, 3, 6 and 9 months) as sub plots were considered. Seed samples were taken randomly from each package at four times period. Essential oil percentage, seed weight, gas composition in packages with modified atmosphere and seed germination factors (seed germination percentage (SGP), germination rate (GR), mean germination time (MGT), and germination Index (GI)) were evaluated during this nine months Storage. Results and Discussion: The highest weight loss was 2/43% in the paper bags stored at 30°C at the fourth to sixth months especially in the fifth month. Aluminum foil package under vacuum condition stored at 30°C had the lowest weight and minimum weight changes during nine months of storage, so it was the best packages compared to others. The results show that Polyethylene-polyamide packages and Aluminum foil packages under vacuum condition are almost impermeable to air and moisture. Increasing weight at 20°C may be due to accumulation of water vapor from the respiration during early period of storage. Seed germination test provides an indication about seedling vigor as well as performance of seed in the field. In most cases, performance relates to the ability of seeds to germinate and produce a seedling that will emerge from the soil and development into a healthy vigorous plant. Packages with different combinations of gas (2% and 10% oxygen) at 30°C,aluminum foil under vacuum condition and Polyethylene-polyamide packages with a gas composition of [98%N2 + 2%O2] at 20°C were packages with higher germination percentage after nine months storage. Based on this results, it appears that packaging materials and storage temperature did not show any significant difference on essential oil percentage and further changes in the amount of oil related to duration of storage. Kumar et al. (2013) showed that the essential oil content and composition were affected by harvest time and storage conditions. Kazaz et al. (2009) investigated the effect of different storage temperatures (0◦C and 3◦C) and durations (7, 14, 21 and 28 days) on oil yield and essential oil components of oil rose (Rosa damascena Mill.). Their results showed that the effect of storage temperatures on oil content were not significant whereas the effect of storage duration was significant and it was similar to our results. Conclusion: Essential oil percentage as the most important property of Carum copticum and germination percentage decreased significantly with increasing of storage period. Finally, results show that Polyethylene-polyamide packages with a gas composition of [98%N2 + 2%O2] and [90%N2 +10%O2] in 9 months storage time and 30±3°C storage temperature preserve qualitative properties better than other conditions. Whereas, aluminum foil package under vacuum condition and 20±3°C storage temperature are recommended for 6 month storage time

Chemical composition and antibacterial activity of methanolic extract and essential oil of Iranian <i>Teucrium polium</i> against some of phytobacteria

<div><p>The antibacterial activity of essential oil and methanolic extract of <i>Teucrium polium</i> was determined against <i>Pseudomonas aeruginosa</i>, <i>Pantoea agglomerans</i>, <i>Brenneria nigrifluens</i>, <i>Rhizobium radiobacter</i>, <i>Rhizobium vitis</i>, <i>Streptomyces scabies</i>, <i>Ralstonia solanacearum</i>, <i>Xanthomonas campestris</i> and <i>Pectobacterium cartovorum</i> by disc diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were determined by using the serial dilution method. Chemical composition of essential oil and methanolic extract was determined by GC-MS and HPLC. α-Pinene (25.769%) and myrcene (12.507) were of the highest percentage in <i>T. polium</i> essential oil, and sinapic acid (15.553 mg/g) and eugenol (6.805 mg/g) were the major compounds in the methanolic extract. Our results indicate that both methanolic extract and essential oil did not show antibacterial activity against <i>P. aeruginosa</i>. Also the essential oil did not show antibacterial activity against <i>P. cartovorum</i>. In general, both methanolic extract and essential oil showed the same antibacterial activity against <i>R. solanacearum</i>, <i>P. agglomerans</i>, <i>B. nigrifluens</i> and <i>S. scabies</i>.</p></div