Chemical composition and antibacterial activity of methanolic extract and essential oil of Iranian <i>Teucrium polium</i> against some of phytobacteria

Abstract

<div><p>The antibacterial activity of essential oil and methanolic extract of <i>Teucrium polium</i> was determined against <i>Pseudomonas aeruginosa</i>, <i>Pantoea agglomerans</i>, <i>Brenneria nigrifluens</i>, <i>Rhizobium radiobacter</i>, <i>Rhizobium vitis</i>, <i>Streptomyces scabies</i>, <i>Ralstonia solanacearum</i>, <i>Xanthomonas campestris</i> and <i>Pectobacterium cartovorum</i> by disc diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were determined by using the serial dilution method. Chemical composition of essential oil and methanolic extract was determined by GC-MS and HPLC. α-Pinene (25.769%) and myrcene (12.507) were of the highest percentage in <i>T. polium</i> essential oil, and sinapic acid (15.553 mg/g) and eugenol (6.805 mg/g) were the major compounds in the methanolic extract. Our results indicate that both methanolic extract and essential oil did not show antibacterial activity against <i>P. aeruginosa</i>. Also the essential oil did not show antibacterial activity against <i>P. cartovorum</i>. In general, both methanolic extract and essential oil showed the same antibacterial activity against <i>R. solanacearum</i>, <i>P. agglomerans</i>, <i>B. nigrifluens</i> and <i>S. scabies</i>.</p></div