Features of Polymeric Structures By Surface—Selective Laser Sintering of Polymer Particles Using Water as Sensitizer

The development of scaffolds with strictly specific properties is a key aspect of functional tissue regeneration, and it still remains one of the greatest challenges for tissue engineering. This study is aimed to determine the possibility of producing three-dimensional polylactide (PLA) scaffolds using the method of surface-selectiv  laser sintering (SSLS) for bone tissue regeneration. In this work, the authors also improved PLA scaffold adhesion properties, which are crucial for successful cellular growth and expansion. Thus, SSLS method proved to be effective in designing threedimensional porous scaffolds with differentiated mechanical properties. Keywords: regenerative medicine, scaffolds, polylactide, surface – selective laser . sintering, tissue engeneering

Laser Printing of Gel Microdrops with Living Cells and Microorganisms

We report the results of experiments on laser printing (wavelength λ=1064 nm) with gel microdrops acting as carriers of living microbial and cellular objects. The dynamics of transport processes with the help of high-speed optical video was studied, which allows to determine characteristics of the formed gel jets and to optimize the operating mode of the laser. It is shown that laser pulses of 4 to 20 ns duration and energy E ≤ 20 μJ should be used to minimize the negative effect on living systems. The results can be used to optimize the technologies of cellular printing and laser engineering of microbial systems (LEMS). LEMS technology is used to isolate hard-cultivated and non-cultivated by classical methods of microorganisms that can act as producers of new biologically active substances and antibiotics. Keywords: laser printing, gel, microdrop, living cell, microbia

Influence of tetrafunctional polylactide morphology on regeneration of bone tissue defects

Определено влияние длины разветвленных цепей сшитых тетрафункциональных полилкатидов на микроморфологию поверхности. Оценены механические свойства структурированных скаффолдов в режиме PeakForce QNM (MultiMode 8, ruker, США). Подобран оптимальный по своим механическим и поверхностным характеристикам тип полилактидов для полной регенерации созданного костного дефекта ткани мышиThe influence of the length of branched chains of cross-linked tetrafunctional polyclatides on the surface micromorphology is determined. The mechanical properties of structured scaffolds are estimated in the PeakForce QNM mode (MultiMode 8, Bruker, USA). Optimal type of polylactide for its mechanical and surface characteristics was selected for complete regeneration of the created bone defect in mouse tissue