ОСОБЛИВОСТІ ЗМІН КАРДІОГЕМОДИНАМІКИ У ПАЦІЄНТІВ ІЗ СТАБІЛЬНОЮ СТЕНОКАРДІЄЮ ТА ГІПОФУНКЦІЄЮ ЩИТОПОДІБНОЇ ЗАЛОЗИ

In patients with angina iodine deficiency can often change metabolism, hemodynamics and autonomic regulation. To find out the features of left ventricular diastolic function in patients with stable angina in combination with subclinical hypothyroidism 2 groups of patients were observed. Among them: 23 patients with coronary artery disease: stable angina FC II-III and 21 patients with stable angina in combination with subclinical hypothyroidism. In all observed patients main indicators of  left ventricular diastolic function were made.In patients with stable angina pectoris in conjunction with subclinical hypothyroidism, comparing to patients with isolated stable angina of corresponding FC, detected more pronounced violation of left ventricular diastolic dysfunction, which more often was manifested by pseudonormal type.У хворих на стенокардію навантаження за умов йододефіциту часто порушуються процеси обміну, вегетативної регуляції та гемодинаміки. Щоб з’ясувати особливості змін діастолічної функції лівого шлуночка у хворих на стабільну стенокардію навантаження у поєднанні з субклінічним гіпотиреозом, обстежено дві ґрупи пацієнтів: 23 – хворих на ішемічну хворобу серця: стабільну стенокардію навантаження ІІ–ІІІ функціонального класу та 21 – на стенокардію у поєднанні з субклінічним гіпотиреозом. Визначали основні показники діастолічної функції лівого шлуночка.Порівняно з пацієнтами з ізольованою стабільною стенокардією навантаження відповідного функціонального класу у хворих на стабільну стенокардію навантаження у поєднанні з субклінічним гіпотиреозом зафіксовано більш виражені прояви діастолічної дисфункції лівого шлуночка, із частішим виявленням псевдонормального типу

ЗМІНИ ВЕГЕТАТИВНОЇ РЕГУЛЯЦІЇ У ХВОРИХ НА СТАБІЛЬНУ СТЕНОКАРДІЮ ТА СУБКЛІНІЧНИЙ ГІПОТИРЕОЗ

In patients with stable angina and subclinical hypothyroidism an imbalance of vegetative regulation can be often developed. 44 patients with stable angina and normal or reduced thyroid gland function were included in the study. The main aim of the study was heart rate variability assessment.In patients with stable angina and subclinical hypothyroidism, TP was 2325.95±49.83 ms2, and the sympatho-vagal balance increased to 2.29±0.04, indicating a decrease of heart rate variability and hypersympathicotonia. In patients with stable angina and normal function of the thyroid gland, these changes were less pronounced and amounted to 3054.30±32.13 ms2 and 2.08±0.06 respectively.У пацієнтів із стабільною стенокардією у поєднанні з субклінічним гіпотиреозом часто виявляють дисбаланс вегетативної регуляції. В ході дослідження обстежено 44 пацієнти із стабільною стенокардією та збереженою або зниженою функцією щитоподібної залози. Вивчали параметри варіабельності ритму серця.У хворих на стенокардію навантаження у поєднанні з субклінічним гіпотиреозом значення ТР становило (2325,95±49,83) мс2, а симпатовагусний індекс зростав і становив 2,29±0,04, що свідчить про зниження варіабельності ритму серця та гіперсимпатикотонію, тоді як у хворих на стенокардію навантаження зі збереженою функцією щитоподібної залози ці зміни були менш вираженими та становили, відповідно, (3054,30±32,13) мс2 і 2,08±0,06

Conditioned medium of induced mesenchymal stem cells as an activator of differentiation in the osteogenic direction

In the prevailing era, the need for new biologically active substances of peptide nature and their therapeutic impacts are of great interest of the contemporary pharmacology. Hence, this study intends to assess the osteoinductive potential of the conditioned medium concentrate on native mesenchymal stem cells. To gratify the study's aim, this study was carried out on a culture of rat mesenchymal stem cells. To stimulate the differentiation of Mesenchymal stem cells in the osteogenic direction, this study used a complete nutrient medium containing dexamethasone, ascorbic acid, sodium beta-glycerophosphate in the concentrations recommended in the guidelines. Subsequently, the resulting concentrate sample was examined using HPLC-MS / MS mass, spectrometric analysis. Part of the previously obtained concentrate of the conditioned medium was used, to assess the proliferation and differentiation of rat Mesenchymal stem cells in the osteogenic direction by staining for alkaline phosphatase. Given the results of the study, it can be concluded that that most of the identified proteins of the conditioned medium concentrate belong to the group of proteins regulating cellular processes, and groups of proteins were also, found that relate to the organization of the extracellular structure, the processes of cell development, or are directly responsible for the differentiation of Mesenchymal stem cells in the osteogenic direction. Along with this, the concentrate of the conditioned medium does not affect the proliferation rate of Mesenchymal stem cells. Thus, the resulting concentrate of the conditioned medium, can be further used, for the development of therapeutic preparations with osteoinductive and regenerative potential

The stop-flow arm equilibrium pressure in preoperative patients: Stressed volume and correlations with echocardiography

Background: The distending intravascular pressure at no flow conditions reflects the stressed volume. While this haemodynamic variable is recognised as clinically important, there is a paucity of reports of its range and responsiveness to volume expansion in patients without cardiovascular disease and no reports of correlations to echocardiographic assessments of left ventricular filling. Methods: Twenty-seven awake (13 male), spontaneously breathing patients without any history of cardiopulmonary, vascular or renal disease were studied prior to induction of anaesthesia. The no-flow equilibrium pressure in the arm following rapid circulatory occlusion (P arm ) was measured via a radial arterial catheter. Transthoracic echocardiography was used to measure left ventricular end diastolic area and volume as well as the diameter of the inferior vena cava. The P arm and echocardiographic variables were measured before and after administration of 500 mL 0.9% NaCl over 10 minutes. Changes were analysed by paired t test, Pearson's correlation and multiple linear regression. Results: P arm increased overall from 22 ± 5 mm Hg to 25 ± 6 mm Hg (mean difference 3.0 ± 4.5 mm Hg, P = 0.002) following the fluid bolus with corresponding increases in arterial pressure and echocardiographic variables. Variability in the direction of the P arm response reflected concomitant changes in vascular compliance. Only weak correlations were observed between changes in P arm and inferior vena cava diameter indexed to body surface area (R 2  = 0.29, P = 0.01). Conclusion: Preoperative measurements of P arm increased following acute expansion of the intravascular volume. Echocardiography demonstrated poor correlation with P arm

New HSV-1 Anti-Viral 1′-Homocarbocyclic Nucleoside Analogs with an Optically Active Substituted Bicyclo[2.2.1]Heptane Fragment as a Glycoside Moiety

New 1′-homocarbanucleoside analogs with an optically active substituted bicyclo[2.2.1]heptane skeleton as sugar moiety were synthesized. The pyrimidine analogs with uracil, 5-fluorouracil, thymine and cytosine and key intermediate with 6-chloropurine (5) as nucleobases were synthesized by a selective Mitsunobu reaction on the primary hydroxymethyl group in the presence of 5-endo-hydroxyl group. Adenine and 6-substituted adenine homonucleosides were obtained by the substitution of the 6-chlorine atom of the key intermediate 5 with ammonia and selected amines, and 6-methoxy- and 6-ethoxy substituted purine homonucleosides by reaction with the corresponding alkoxides. No derivatives appeared active against entero, yellow fever, chikungunya, and adeno type 1viruses. Two compounds (6j and 6d) had lower IC50 (15 ± 2 and 21 ± 4 µM) and compound 6f had an identical value of IC50 (28 ± 4 µM) to that of acyclovir, suggesting that the bicyclo[2.2.1]heptane skeleton could be further studied to find a candidate for sugar moiety of the nucleosides

New HSV-1 Anti-Viral 1 '-Homocarbocyclic Nucleoside Analogs with an Optically Active Substituted Bicyclo[2.2.1]Heptane Fragment as a Glycoside Moiety

New 1'-homocarbanucleoside analogs with an optically active substituted bicyclo[2.2.1]heptane skeleton as sugar moiety were synthesized. The pyrimidine analogs with uracil, 5-fluorouracil, thymine and cytosine and key intermediate with 6-chloropurine (5) as nucleobases were synthesized by a selective Mitsunobu reaction on the primary hydroxymethyl group in the presence of 5-endo-hydroxyl group. Adenine and 6-substituted adenine homonucleosides were obtained by the substitution of the 6-chlorine atom of the key intermediate 5 with ammonia and selected amines, and 6-methoxy- and 6-ethoxy substituted purine homonucleosides by reaction with the corresponding alkoxides. No derivatives appeared active against entero, yellow fever, chikungunya, and adeno type 1viruses. Two compounds (6j and 6d) had lower IC50 (15 ± 2 and 21 ± 4 µM) and compound 6f had an identical value of IC50 (28 ± 4 µM) to that of acyclovir, suggesting that the bicyclo[2.2.1]heptane skeleton could be further studied to find a candidate for sugar moiety of the nucleosides.status: publishe

Discovery of New Ginsenol-Like Compounds with High Antiviral Activity

A number of framework amides with a ginsenol backbone have been synthesized using the Ritter reaction. We named the acetamide as Ginsamide. A method was developed for the synthesis of the corresponding amine and thioacetamide. The new compounds revealed a high activity against H1N1 influenza, which was confirmed using an animal model. Biological experiments were performed to determine the mechanism of action of the new agents, a ginsamide-resistant strain of influenza virus was obtained, and the pathogenicity of the resistant strain and the control strain was studied. It was shown that the emergence of resistance to Ginsamide was accompanied by a reduction in the pathogenicity of the influenza virus