The analysis of methylation of DNA promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium

The hyperplastic processes of the endometrium can arise not only against the background of excessive influence of estrogen, but also against the background of epigenetic damages that affect apoptosis, cell proliferation, differentiation, and adhesion, and DNA reparation. The aim of our study was to investigate and analyze the status of methylation of the promoter of SFRP2 gene in patients with hyperplastic processes of the endometrium. Materials and Methods: The study groups were the following: I — patients with endometrial hyperplasia (EH, n = 9); II — patients with endometrial intraepithelial neoplasia (EIN, n = 10), III — control groups: 1) with endometrial cancer (EC, n = 4), and 2) healthy women (n = 4). Determination of promoter methylation of SFRP2 gene was carried out by the semiquantitative method of methylation-specific PCR assay. Results: The maximum level of methylation of SFRP2 gene promoter had been revealed in patients with EC — 42.80 ± 3.55% (р < 0.05). The patients of the I group had the lowest values of methylation of SFRP2 gene promoter — 10.66 ± 0.85%, while in patients of the II group this indicator was higher — 20.60 ± 0.95% (р < 0.05). In healthy women of the control group, methylation of SFRP2 gene promoter was detected in none of the samples. Conclusion: The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20–25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients

Аналіз експресії протоонкогену STAT3 у жінок із передраковими ураженнями шийки матки

Обстежено 90 жінок віком від 25 до 45 років (середній вік 36,4±1,3 років). Всі пацієнтки проходили загальноприйняте клінічне, гінекологічне, інструментальне та лабораторне обстеження на базі МКЛ № 9 ім. проф. О. І. Мінакова, згідно чинних клінічних протоколів. Стан шийки матки оцінювали за допомогою цитологічного, кольпоскопічного досліджень, з подальшим патогістологічним дослідженням біопсійного матеріалу, який був взятий з піхвової частини шийки матки після проведення кольпоскопічного дослідження та виконання гістеректомій з 2010 по 2013 рік. Наявність генітальної папіломавірусної інфекції, визначення типу вірусу проводилось методом полімеразної ланцюгової реакції у реальному часі. Було зібрано 90біоптатів шийки матки, серед яких 70 передракових (CIN I – 10 (14,3%), CIN II – 32 (45,7%), CIN III – 28 (40,0%)) та 20 нормальних контрольних зразків. Частина кожного зі зразків, була поміщена в охолоджений 1 фосфатний буфер сольового розчину (PBS), які відправлені для молекулярно-біологічних робіт, а друга половина для патогістологічного дослідження в розчині формаліну. Патогістологічний діагноз встановлений згідно класифікації Всесвітньої організації Охорони Здоров’я

Digital receivers for low-frequency radio telescopes UTR-2, URAN, GURT

This paper describes digital radio astronomical receivers used for decameter and meter wavelength observations. This paper describes digital radio astronomical receivers used for decameter and meter wavelength observations. Since 1998, digital receivers performing on-the-fly dynamic spectrum calculations or waveform data recording without data loss have been used at the UTR-2 radio telescope, the URAN VLBI system, and the GURT new generation radio telescope. Here we detail these receivers developed for operation in the strong interference environment that prevails in the decameter wavelength range. Data collected with these receivers allowed us to discover numerous radio astronomical objects and phenomena at low frequencies, a summary of which is also presented.Comment: 24 pages, 15 figure

Comparison of the results of surgical treatment of patients with Achilles tendon injury at Myerson stage II and III diastasis using plantar muscle flexor tendon

Single-center randomized controlled partially blinded research aimed at the determination of functional results of surgical treatment of patients with Achilles tendon injury of different Myerson stages was carried out in the clinic of Irkutsk Scientific Centre of Surgery and Traumatology. Based on the analysis of surgical treatment of 20 patients with Achilles tendon divided into two groups (with Myerson stage 11 and stage 111 diastasis) we showed the effectiveness of surgical treatment using plantar muscle flexor tendon. We assessed the following criteria: 1) time from the moment of injury, diastasis length, duration of surgery; 2) pain syndrome after the surgery, omission of additional mobility aids and equipment; 3) AOFAS functional results. While the analysis we found statistically significant differences in studied groups by the criteria "time from the moment of injury" and "diastasis length". As the result of the research we found statistically significant differences by "duration of surgery» criterion. In consequence of using plantar muscle flexor tendon as a stabilizing factor at the restoration of Achilles tendon we didn't register the differences between the groups by the level and duration of pain syndrome after the surgery, terms of the omission of additional mobility aids and equipment after the surgery and also in AOFAS functional results

A study on L-threonine and L-serine uptake in Escherichia coli K-12

In the current study, we report the identification and characterization of the yifK gene product as a novel amino acid carrier in E. coli K-12 cells. Both phenotypic and biochemical analyses showed that YifK acts as a permease specific to L-threonine and, to a lesser extent, L-serine. An assay of the effect of uncouplers and composition of the reaction medium on the transport activity indicates that YifK utilizes a proton motive force to energize substrate uptake. To identify the remaining threonine carriers, we screened a genomic library prepared from the yifK-mutant strain and found that brnQ acts as a multicopy suppressor of the threonine transport defect caused by yifK disruption. Our results indicate that BrnQ is directly involved in threonine uptake as a low-affinity but high-flux transporter, which forms the main entry point when the threonine concentration in the external environment reaches a toxic level. By abolishing YifK and BrnQ activity, we unmasked and quantified the threonine transport activity of the LIV-I branched chain amino acid transport system and demonstrated that LIV-I contributes significantly to total threonine uptake. However, this contribution is likely smaller than that of YifK. We also observed the serine transport activity of LIV-I, which was much lower compared with that of the dedicated SdaC carrier, indicating that LIV-I plays a minor role in the serine uptake. Overall, these findings allow us to propose a comprehensive model of the threonine/serine uptakesubsystem in E. coli cells

Primary plasty of an Achilles tendon by plantar muscle tendon (clinical case)

Patient T., 43 years old arrived in clinic with complaints to the pains in lower thir of the right shin, limitation of movements in right ankle joint, inability of using right lower extremity. An. operative treatment was realized in clinic: suture of rupture of Achilles tendon by Cuneo with plastics by the tendon of long plantar muscle. In 4 weeks plaster immobilization was removed, the patient started measured loading of operated extremity with additional cane support. In 6 weeks full load on operated extremity was allowed and the patient returned to work. In 2 months patient received repeated trauma. During the examination local painfulness in the area of operative intervention was revealed. At the palpation it was revealed that integrity of plantar muscle tendon isn't broken. Control MRR of right shin was made: as the result of control examination the injury of plantar muscle tendon wasn't revealed. Immobilization of operated extremity in neutral position of a foot by functional brace was recommended to the patient. Measured, loading with additional additional cane support was approved. In 2 weeks the immobilization was removed, function of operated extremity is fully restored. Conclusion: if proposed method allows to start earlier loadings and. to decrease possibility of repeated rupture of reconstructed plantar muscle tendon in the most active period of rehabilitation