Slowing heavy, ground-state molecules using an alternating gradient decelerator

Cold supersonic beams of molecules can be slowed down using a switched sequence of electrostatic field gradients. The energy to be removed is proportional to the mass of the molecules. Here we report deceleration of YbF, which is 7 times heavier than any molecule previously decelerated. We use an alternating gradient structure to decelerate and focus the molecules in their ground state. We show that the decelerator exhibits the axial and transverse stability required to bring these molecules to rest. Our work significantly extends the range of molecules amenable to this powerful method of cooling and trapping.Comment: 4 pages, 5 figure

Temperature effect in the Casimir attraction of a thin metal film

The Casimir effect for conductors at arbitrary temperatures is theoretically studied. By using the analytical properties of the Green functions and applying the Abel-Plan formula to Lifshitz's equation, the Casimir force is presented as sum of a temperature dependent and vacuum contributions of the fluctuating electromagnetic field. The general results are applied to the system consisting of a bulk conductor and a thin metal film. It is shown that a characteristic frequency of the thermal fluctuations in this system is proportional to the square root of a thickness of the metal film. For the case of the sufficiently high temperatures when the thermal fluctuations play the main role in the Casimir interaction, this leads to the growth of the effective dielectric permittivity of the film and to a disappearance of the dependence of Casimir's force on the sample thickness.Comment: LaTeX 2.09, 8 pages, no figure

Nuclear fragmentation: sampling the instabilities of binary systems

We derive stability conditions of Asymmetric Nuclear Matter ($ANM$) and discuss the relation to mechanical and chemical instabilities of general two-component systems. We show that the chemical instability may appear as an instability of the system against isoscalar-like rather than isovector-like fluctuations if the interaction between the two constituent species has an attractive character as in the case of $ANM$. This leads to a new kind of liquid-gas phase transition, of interest for fragmentation experiments with radioactive beams.Comment: 4 pages (LATEX), 3 Postscript figures, improved version, added reference

A hydroquinone-specific screening system for directed P450 evolution

The direct hydroxylation of benzene to hydroquinone (HQ) under mild reaction conditions is a challenging task for chemical catalysts. Cytochrome P450 (CYP) monooxygenases are known to catalyze the oxidation of a variety of aromatic compounds with atmospheric dioxygen. Protein engineering campaigns led to the identification of novel P450 variants, which yielded improvements in respect to activity, specificity, and stability. An effective screening strategy is crucial for the identification of improved enzymes with desired characteristics in large mutant libraries. Here, we report a first screening system designed for screening of P450 variants capable to produce hydroquinones. The hydroquinone quantification assay is based on the interaction of 4-nitrophenylacetonitrile (NpCN) with hydroquinones under alkaline conditions. In the 96-well plate format, a low detection limit (5 μM) and a broad linear detection range (5 to 250 μM) were obtained. The NpCN assay can be used for the quantification of dihydroxylated aromatic compounds such as hydroquinones, catechols, and benzoquinones. We chose the hydroxylation of pseudocumene by P450 BM3 as a target reaction and screened for improved trimethylhydroquinone (TMHQ) formation. The new P450 BM3 variant AW2 (R47Q, Y51F, I401M, A330P) was identified by screening a saturation mutagenesis library of amino acid position A330 with the NpCN assay. In summary, a 70-fold improved TMHQ formation was achieved with P450 BM3 AW2 when compared to the wild type (WT) and a 1.8-fold improved TMHQ formation compared to the recently reported P450 BM3 M3 (R47S, Y51W, A330F, I401M). © 2018, The Author(s)

Darboux transformations for a 6-point scheme

We introduce (binary) Darboux transformation for general differential equation of the second order in two independent variables. We present a discrete version of the transformation for a 6-point difference scheme. The scheme is appropriate to solving a hyperbolic type initial-boundary value problem. We discuss several reductions and specifications of the transformations as well as construction of other Darboux covariant schemes by means of existing ones. In particular we introduce a 10-point scheme which can be regarded as the discretization of self-adjoint hyperbolic equation

Measurement of the electron electric dipole moment using YbF molecules

The most sensitive measurements of the electron electric dipole moment d_e have previously been made using heavy atoms. Heavy polar molecules offer a greater sensitivity to d_e because the interaction energy to be measured is typically 10^3 times larger than in a heavy atom. We report the first measurement of this kind, for which we have used the molecule YbF. Together, the large interaction energy and the strong tensor polarizability of the molecule make our experiment essentially free of the systematic errors that currently limit d_e measurements in atoms. Our first result d_e = (- 0.2 \pm 3.2) x 10^-26 e.cm is less sensitive than the best atom measurement, but is limited only by counting statistics and demonstrates the power of the method.Comment: 4 pages, 4 figures. v2. Minor corrections and clarifications made in response to referee comment

Cascade Failure in a Phase Model of Power Grids

We propose a phase model to study cascade failure in power grids composed of generators and loads. If the power demand is below a critical value, the model system of power grids maintains the standard frequency by feedback control. On the other hand, if the power demand exceeds the critical value, an electric failure occurs via step out (loss of synchronization) or voltage collapse. The two failures are incorporated as two removal rules of generator nodes and load nodes. We perform direct numerical simulation of the phase model on a scale-free network and compare the results with a mean-field approximation.Comment: 7 pages, 2 figure