A Temporal Web Ontology Language

The Web Ontology Language (OWL) is the most expressive standard language for modeling ontologies on the Semantic Web. In this paper, we present a temporal extension of the very expressive fragment SHIN(D) of the OWL-DL language resulting in the tOWL language. Through a layered approach we introduce 3 extensions: i) Concrete Domains, that allows the representation of restrictions using concrete domain binary predicates, ii) Temporal Representation, that introduces timepoints, relations between timepoints, intervals, and Allen’s 13 interval relations into the language, and iii) TimeSlices/Fluents, that implements a perdurantist view on individuals and allows for the representation of complex temporal aspects, such as process state transitions. We illustrate the expressiveness of the newly introduced language by providing a TBox representation of Leveraged Buy Out (LBO) processes in financial applications and an ABox representation of one specific LBO

Addressing health information privacy with a novel cloud-based PHR system architecture

Patient Health Records (PHRs) shift the ownership of health data from health providers to patients. Such a shift poses important challenges from the data privacy point of view. Patients would like to be able to selectively reveal information to other stakeholders and, at the same time, be assured that their health information will not be used improperly once shared. Current PHR systems partially fail to satisfy these requirements. In this paper, we show that both requirements can be satisfied fully when adopting a novel cloud-based PHR system architecture.We expain the role of remote virtual machines in this architecture and use interaction models to reason about privacy implications. Finally, we evaluate MyPHRMachines, a prototypical implementation of the architecture: we demonstrate that the system enables the execution of third party genome analysis services on patientowned genome data while ensuring that (1) such services cannot maliciously store this data and (2) patients can show the analysis results to experts without sharing along their full genome

The European Union, borders and conflict transformation: the case of Cyprus

Much of the existing literature on the European Union (EU), conflict transformation and border dynamics has been premised on the assumption that the nature of the border determines EU intervention and the consequences that flow from this in terms of EU impact. The article aims to transcend this literature through assessing how domestic interpretations influence EU border transformation in conflict situations, taking Cyprus as a case study. Moreover, the objective is to fuse the literature on EU bordering impact and perceptions of the EU’s normative projection in conflict resolution. Pursuing this line of inquiry is an attempt to depart from the notion of borders being constructed solely by unidirectional EU logics of engagement or bordering practices to a conceptualization of the border as co-constituted space, where the interpretations of the EU’s normative projections by conflict parties, and the strategies that they pursue, can determine the relative openness of the EU border

Extreme ionization of heavy atoms in solid-density plasmas by relativistic second-harmonic laser pulses

Stripping heavy atoms in solid matter of most of their electrons requires the extreme conditions that exist in astrophysical plasmas, but are difficult to create in the laboratory1–3. Here we demonstrate solid-density gold plasmas with atoms stripped of up to 72 electrons (N-like Au72+) over large target depths. This record ionization is achieved by irradiating solid foils and near-solid-density nanowire arrays with highly relativistic (3 × 1021 W cm−2) second-harmonic femtosecond laser pulses of '10 J energy focused into a 1.6 µm spot. The short wavelength and high intensity enable the interaction to occur at a relativistic critical density4,5 of 1023 cm−3. Solid targets reach a higher average charge in 1- to 2-µm-thick layers, while the less dense nanowire plasmas are heated to much larger depths ('8 µm) by energetic electrons generated near the nanowire tips. Larger laser spots could result in solid Au plasmas ionized up to He-like.Fil: Hollinger, R.. State University of Colorado - Fort Collins; Estados UnidosFil: Wang, S.. State University of Colorado - Fort Collins; Estados UnidosFil: Wang, Y.. State University of Colorado - Fort Collins; Estados UnidosFil: Moreau, A.. State University of Colorado - Fort Collins; Estados UnidosFil: Capeluto, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Song, H.. State University of Colorado - Fort Collins; Estados UnidosFil: Rockwood, A.. State University of Colorado - Fort Collins; Estados UnidosFil: Bayarsaikhan, E.. State University of Colorado - Fort Collins; Estados UnidosFil: Kaymak, V.. Universitat Dusseldorf; AlemaniaFil: Pukhov, A.. Universitat Dusseldorf; AlemaniaFil: Shlyaptsev, V.N.. State University of Colorado - Fort Collins; Estados UnidosFil: Rocca, J.J.. State University of Colorado - Fort Collins; Estados Unido

Enhanced electron acceleration in aligned nanowire arrays irradiated at highly relativistic intensities

We report a significant enhancement in both the energy and the flux of relativistic electrons accelerated by ultra-intense laser pulse irradiation (>1 10 21 W cm-2) of near solid density aligned CD2 nanowire arrays in comparison to those from solid CD2 foils irradiated with the same laser pulses. Ultrahigh contrast femtosecond laser pulses penetrate deep into the nanowire array creating a large interaction volume. Detailed three dimensional relativistic particle-in-cell simulations show that electrons originating anywhere along the nanowire length are first driven towards the laser to reach a lower density plasma region near the tip of the nanowires, where they are accelerated to the highest energies. Electrons that reach the lower density plasma experience direct laser acceleration up to the dephasing length, where they outrun the laser pulse. This yields an electron beam characterized by a 3 higher electron temperature and an integrated flux 22.4 larger respect to foil targets. Additionally, the generation of >1 MeV photons were observed to increase up to 4.5.Fil: Moreau, A.. State University of Colorado - Fort Collins; Estados UnidosFil: Hollinger, R.. State University of Colorado - Fort Collins; Estados UnidosFil: Calvi, C.. State University of Colorado - Fort Collins; Estados UnidosFil: Wang, S.. State University of Colorado - Fort Collins; Estados UnidosFil: Wang, Y.. State University of Colorado - Fort Collins; Estados UnidosFil: Capeluto, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Rockwood, A.. State University of Colorado - Fort Collins; Estados UnidosFil: Curtis, A.. State University of Colorado - Fort Collins; Estados UnidosFil: Kasdorf, S.. State University of Colorado - Fort Collins; Estados UnidosFil: Shlyaptsev, V.N.. State University of Colorado - Fort Collins; Estados UnidosFil: Kaymak, V.. Universitat Dusseldorf; AlemaniaFil: Pukhov, A.. Universitat Dusseldorf; AlemaniaFil: Rocca, J.J.. State University of Colorado - Fort Collins; Estados Unido

Amino acid availability acts as a metabolic rheostat to determine the magnitude of ILC2 responses

Group 2 innate lymphoid cells (ILC2) are functionally poised, tissue-resident lymphocytes that respond rapidly to damage and infection at mucosal barrier sites. ILC2 reside within complex microenvironments where they are subject to cues from both the diet and invading pathogens—including helminths. Emerging evidence suggests ILC2 are acutely sensitive not only to canonical activating signals but also perturbations in nutrient availability. In the context of helminth infection, we identify amino acid availability as a nutritional cue in regulating ILC2 responses. ILC2 are found to be uniquely preprimed to import amino acids via the large neutral amino acid transporters Slc7a5 and Slc7a8. Cell-intrinsic deletion of these transporters individually impaired ILC2 expansion, while concurrent loss of both transporters markedly impaired the proliferative and cytokine-producing capacity of ILC2. Mechanistically, amino acid uptake determined the magnitude of ILC2 responses in part via tuning of mTOR. These findings implicate essential amino acids as a metabolic requisite for optimal ILC2 responses within mucosal barrier tissues

Treatment of acne with intermittent and conventional isotretinoin: a randomized, controlled multicenter study

Oral isotretinoin is the most effective choice in the treatment of severe acne. Application of isotretionin to acne has been expanded to treat those patients with less severe but scarring acne who are responding unsatisfactorily to conventional therapies. However, its use is associated with many side effects, some of which can result in very disastrous consequences. Data related with intermittent isotretinoin therapy is still limited. Our aim was to asses the efficacy and tolerability of two different intermittent isotretinoin courses and compare them with conventional isotretinoin treatment. In this multicenter and controlled study, 66 patients with moderate to severe cases were randomized to receive either isotretionin for the first 10 days of each month for 6 months (group 1), or each day in the first month, afterwards the first 10 days of each month for 5 months (group 2) or daily for 6 months (group 3). The drug dosage was 0.5 mg/kg/day in all groups. Patients were followed-up for 12 months. Efficacy values were evaluable for 22 patients in group 1, 19 patients in group 2, and 19 patients in group 3. Acne scores in each group were significantly lower at the end of treatment and follow-up periods (P < 0.001). When patients were evaluated separately as moderate (n = 31) and severe (n = 29), no statistically significant differences were obtained among the treatment protocols in patients with moderate acne. However, there was a significant difference between groups 1 and 3 to the response of the treatments in severe acne patients at the end of follow-up period (P = 0.013). The frequency and severity of isotretionin-related side effects were found to be lower in groups 1 and 2 compared with group 3. Intermittent isotretinoin may represent an effective alternative treatment, especially in moderate acne with a low incidence and severity of side effects. The intermittent isotretinoin can be recommended in those patients not tolerating the classical dosage

Predicting RNA-Protein Interactions Using Only Sequence Information

<p>Abstract</p> <p>Background</p> <p>RNA-protein interactions (RPIs) play important roles in a wide variety of cellular processes, ranging from transcriptional and post-transcriptional regulation of gene expression to host defense against pathogens. High throughput experiments to identify RNA-protein interactions are beginning to provide valuable information about the complexity of RNA-protein interaction networks, but are expensive and time consuming. Hence, there is a need for reliable computational methods for predicting RNA-protein interactions.</p> <p>Results</p> <p>We propose <b><it>RPISeq</it></b>, a family of classifiers for predicting <b><it>R</it></b>NA-<b><it>p</it></b>rotein <b><it>i</it></b>nteractions using only <b><it>seq</it></b>uence information. Given the sequences of an RNA and a protein as input, <it>RPIseq </it>predicts whether or not the RNA-protein pair interact. The RNA sequence is encoded as a normalized vector of its ribonucleotide 4-mer composition, and the protein sequence is encoded as a normalized vector of its 3-mer composition, based on a 7-letter reduced alphabet representation. Two variants of <it>RPISeq </it>are presented: <it>RPISeq-SVM</it>, which uses a Support Vector Machine (SVM) classifier and <it>RPISeq-RF</it>, which uses a Random Forest classifier. On two non-redundant benchmark datasets extracted from the Protein-RNA Interface Database (PRIDB), <it>RPISeq </it>achieved an AUC (Area Under the Receiver Operating Characteristic (ROC) curve) of 0.96 and 0.92. On a third dataset containing only mRNA-protein interactions, the performance of <it>RPISeq </it>was competitive with that of a published method that requires information regarding many different features (e.g., mRNA half-life, GO annotations) of the putative RNA and protein partners. In addition, <it>RPISeq </it>classifiers trained using the PRIDB data correctly predicted the majority (57-99%) of non-coding RNA-protein interactions in NPInter-derived networks from <it>E. coli, S. cerevisiae, D. melanogaster, M. musculus</it>, and <it>H. sapiens</it>.</p> <p>Conclusions</p> <p>Our experiments with <it>RPISeq </it>demonstrate that RNA-protein interactions can be reliably predicted using only sequence-derived information. <it>RPISeq </it>offers an inexpensive method for computational construction of RNA-protein interaction networks, and should provide useful insights into the function of non-coding RNAs. <it>RPISeq </it>is freely available as a web-based server at <url>http://pridb.gdcb.iastate.edu/RPISeq/.</url></p

Evidence, and replication thereof, that molecular-genetic and environmental risks for psychosis impact through an affective pathway

Background There is evidence that environmental and genetic risk factors for schizophrenia spectrum disorders are transdiagnostic and mediated in part through a generic pathway of affective dysregulation. Methods We analysed to what degree the impact of schizophrenia polygenic risk (PRS-SZ) and childhood adversity (CA) on psychosis outcomes was contingent on co-presence of affective dysregulation, defined as significant depressive symptoms, in (i) NEMESIS-2 (n = 6646), a representative general population sample, interviewed four times over nine years and (ii) EUGEI (n = 4068) a sample of patients with schizophrenia spectrum disorder, the siblings of these patients and controls. Results The impact of PRS-SZ on psychosis showed significant dependence on co-presence of affective dysregulation in NEMESIS-2 [relative excess risk due to interaction (RERI): 1.01, p = 0.037] and in EUGEI (RERI = 3.39, p = 0.048). This was particularly evident for delusional ideation (NEMESIS-2: RERI = 1.74, p = 0.003; EUGEI: RERI = 4.16, p = 0.019) and not for hallucinatory experiences (NEMESIS-2: RERI = 0.65, p = 0.284; EUGEI: -0.37, p = 0.547). A similar and stronger pattern of results was evident for CA (RERI delusions and hallucinations: NEMESIS-2: 3.02, p < 0.001; EUGEI: 6.44, p < 0.001; RERI delusional ideation: NEMESIS-2: 3.79, p < 0.001; EUGEI: 5.43, p = 0.001; RERI hallucinatory experiences: NEMESIS-2: 2.46, p < 0.001; EUGEI: 0.54, p = 0.465). Conclusions The results, and internal replication, suggest that the effects of known genetic and non-genetic risk factors for psychosis are mediated in part through an affective pathway, from which early states of delusional meaning may arise