Pattern of tuberculosis among tribal population of Central India with special reference to cartridge based nucleic acid amplification test as diagnostic tool: a descriptive study at tertiary care hospital

Background: Tuberculosis (TB) kills close to half a million Indians every year. Lack of reliable rapid diagnostic techniques for TB hampers timely diagnosis and leads to continued disease transmission, causing significant morbidity and mortality. The potential of newly recommended CBNAAT in TB and MDR-TB detection has been underutilized in our area due to lack of awareness regarding the same. Hence we utilized this rapid, logistically simplified test to study the pattern of tuberculosis among tribal population of Central India.Methods: Descriptive study of suspected TB patients in tertiary care centre from March 2016 to March 2019. Appropriate specimens from suspected TB patients were collected and subjected to CBNAAT and AFB smear to study the pattern of TB and Rifampicin- Resistant(RR) TB  in our area.Results: CBNAAT detected overall 27% MTB cases; 27.72 % Pulmonary-TB cases as against smear positivity rate of 20.73% whereas 12.74% Extra-pulmonary-TB (EPTB) cases as against smear positivity rate of 1.59%.Overall 94.91% were RiF Sensitive( RS-TB) and 4.58% were RR-TB. Of the 57 (4.16%) HIV-TB coinfected cases; 96.49% were RS-TB and 5.26% were RR-TB. Co-infected patients have high incidence of EPTB(21.05%) involvement  with RR-TB 3.50%. Among EPTB cases; lymph node aspirate and pus provided highest CBNAAT positive cases and almost 90.62% EPTB specimens were RS-TB .Conclusions: Availability of new diagnostic services has increased early identification of TB and RR-TB. Awareness among physicians regarding diagnostic utility of CBNAAT should be further increased as early identification of possible MDR cases is key to reducing community transmission and treatment initiation, particularly in high-burden, resource-limited settings. 

Evaluation of nephroprotective effect of Bryonia lacinosa on streptozotocin induced diabetic nephropathy in rats

Background: Ayurvedic literature indicated use of Bryonia laciniosa (BL) for metabolic abnormalities. However only one study evaluated Bryonia laciniosa seed ethanolic extract in streptozotocin-induced diabetes in rats and was found to have anti-hyperglycemic and anti-hyperlipidemic effects. The authors looked at the renal function test in addition and found that it prevents deterioration of renal function in rats. Hence it was interesting to find if Bryonia laciniosa had any protective action in streptozotocin induced diabetic nephropathy in rats.Methods: Streptozotocin induced diabetic nephropathy model in wistar rats was standardised in phase 1. In phase 2 animals were divided into 4 groups and diabetic nephropathy was induce with STZ. Vehicle Control group animals received CMC 0.5%, Positive control group received enalapril 5 mg/kg, Test group 1 received ethanolic extract of Bryonia laciniosa (EEBL) 250 mg/kg and Test group 2 received (EEBL) 500 mg/kg from 3rd day onwards till 6 weeks. Renal function, morphology and histopthological changes were evaluated.Results: There was a significant reduction (p <0.001) in the serum creatinine and BUN in both low and high dose EEBL groups. High dose of EEBL lowered raised MDA values significantly (p<0.001) and raised the GSH values (p<0.001). On histopathology, the high dose EEBL reversed nephropathic changes which were equivalent to enalapril.Conclusions: Bryonia lacinosa in high doses 500 mg/kg has potential to reverse diabetic nephropathic changes induced in wistar rats

To evaluate and compare the efficacy of alcoholic and aqueous extract of Lagenaria siceraria in high fat diet model in wistar rats

Background: Obesity is not only affecting the affluent society but also affecting developing countries like India. The incidence of obesity is rapidly increasing throughout the world. However, the current anti-obesity drugs have numerous limitations.Methods: The obesity was induced in male wistar rats by giving high-fat diet over 12 weeks. The variables assessed were body weight, abdominal girth, blood triglyceride level, liver weight and fat mass and histopathology of liver. Aqueous and alcoholic extracts of Lagenaria siceraria (200mg/kg and 400mg/kg Doses) were compared to orlistat (treatment control) and high-fat diet group (disease control) for different variables.Results: Alcoholic and aqueous extracts high dose (400mg/kg) of Lagenaria siceraria significantly reduced total body weight (p&lt;0.05), abdominal girth (p &lt;0.05) at week 10 and 12 compared to high fat diet group. Alcoholic extract (400mg/kg) significantly reduced total blood triglyceride level (p &lt;0.05) and total liver weight (p &lt;0.05) compared to high-fat diet group. None of the study drugs reduced % liver weight. Alcoholic extract high dose (p &lt;0.05) has shown improvement in histopathological score. Both aqueous and alcoholic extracts have shown reduced fat mass compared to high-fat diet group.Conclusions: The alcoholic extract (400mg/kg) of Lagenaria siceraria alleviated high fat diet induced obesity and dyslipidemic changes in rats. The alcoholic extract of Lagenaria siceraria is having better anti-obesity potential than aqueous extract

Anti-inflammatory and analgesic activity of different fractions of Boswellia serrata

The study was designed to investigate the anti-inflammatory and analgesic effect of different fractions of Boswellia serrata. The effect of different fractions of Boswellia serrata were studied using carrageenan induced paw edema, acetic acid induced writhing response, formalin induced pain, hot plate and tail flick method for studying anti-inflammatory and analgesic activity, respectively. The different fractions of B. serrata, essential oil (10 ml/kg), gum (100 mg/kg, resin (100 mg/kg) oleo-resin (100 mg/kg) and oleo-gum-resin (100 mg/kg) significantly reduces carrageenan induced inflammation in rats and shows analgesic activity, as determined by acetic acid induced writhing response, formalin induced pain, hot plate and tail flick method. The different fractions of B. serrata showed prompt anti-inflammatory and analgesic activity due to the inhibition of 5-lipoxygenase enzyme.Keywords: Analgesic; Boswellia serrata; Inflammation; 5- lipoxygenase; Burseraceae

Developing linkages for agricultural technology transfer: A case study of research institution and voluntary organization partnership

A paradigm shift to the private and voluntary organizations led extension is being witnessed as the public agricultural extension system has been adversely affected by various constraints. ICAR-Indian Agricultural Research Institute (IARI) a premier agricultural research institution took lead to develop linkages with voluntary organizations (VOs) of repute for speedy transfer of agricultural technologies to the ultimate users. The present study was conducted on randomly selected 120 farmers of Patiala district to analyze the effectiveness of IARI linkage with VO namely Young Farmers' Association (YFA) of Punjab state. The advisory services through IARI-YFA linkage, higher yield of IARI released varieties, social participation of farmers and their education were the major determinants for the adoption of IARI varieties. In case of both rice and wheat, IARI varieties were sown in more than 60% area of the total land holdings of the farmers. Although, the linkage was perceived highly effective in terms of the delivery of farm technologies, advisory services, farm literature supplied, farmers' fair, front line demonstrations, impact on yield and income of farmers and satisfaction of farmers but non-availability of seeds in desired quantity, higher seed cost and less land for seed multiplication were major inhibiting factors. The functional efficacy of linkage may be enhanced through close collaboration of state level government and private agencies for broad based, inclusive and sustainable agricultural development

Present status and future prospects of Gir cow

Gir cattle in Saurashtra provide milk, manure and draught power. Animal genetic resources of native breeds are largely underutilized in India and indiscriminate crossbreeding has degraded large numbers of local breeds. This is also true with Gir cattle in its native breeding tract. (i) About two-third population is either graded Gir or Gir like and hence not pure Gir, (ii) only one-third population i.e. 7.6 lakh could be 95% towards pure Gir. Hence, it is difficult to obtain large number of pure Gir animals. Since 2003–04 to 2012– 13, contribution of Saurashtra to state total indigenous cattle milk yield is 60%. A 25 years data analysis on Gir cows at CBF revealed average lactation yield as 2029 litre in 321 days lactation length and 1843.8 litre milk yield in 300 days. Production performance of Gir cows in three districts:(1) Junagadh (2) Rajkot and (3) Bhavnagar showed that average test day milk yield in Gir cows over a lactation were 7.99±0.05 litre/d for Junagadh, 6.43±0.03 litre/d for Rajkot and 6.71±0.04 litre /d for Bhavnagar district, projected values for 300-days milk yield being was 2397, 1929, and 2013 litre for the three districts respectively. Average AFC in Gir cattle of the station herd was 1527.7±14.1 days (51 mth) whereas survey study in Junagadh, Rajkot and Bhavnagar districts indicated AFC in field Gir cows as 45, 49, and 46 month, respectively. Parity had a significant effect on total lactation milk yield in Gir cows at CBF. Highest total lactation yield was observed in 2nd lactation (2132±123.4 litre) while lowest was observed in 10th lactation (1456.8±219.1 litre). Milk production traits LY and 300-d LY exhibited 10 to 13 % genetic variability in the herd. This suggests that to increase variability in the herd, genetic material from outside herd needs to be introduced

Comparative Evaluation of ICP-AES and Turbidimetric Methods for Determining Extractable Sulfur in Soils

The deficiency of sulfur (S) as a constraint to crop productivity in irrigated, intensified systems has long been recognized (Kanwar 1972; Pasricha and Fox 1993; Singh 2001). A recent survey of farmers’ fields in the Indian semi-arid tropics (SAT) demonstrated that the deficiency of S, as a constraint to crop production and productivity, is also equally widespread in the rainfed production systems (Rego et al. 2007; Sahrawat et al. 2007). The results of this research further showed that soil testing was effective in diagnosing S deficiency; and the crops grown on farmers’ fields with calcium chloride extractable-S levels of less than 8-10 mg kg-1 soil responded positively to the application of sulfur (Rego et al. 2007)

Comparative Evaluation of Inductively Coupled Plasma–Atomic Emission Spectroscopy and Colorimetric Methods for Determining Hot-Water-Extractable Boron in Soils

Frequency of boron (B) deficiency is increasing in rainfed systems, and hence there is a need to diagnose the deficiency. Colorimetric methods are still widely used in soil-testing laboratories in India for measuring B. Little information is available on the comparative evaluation of the colorimetric and inductively coupled plasma (ICP) methods for determining extractable B in soils. We describe results of the comparative evaluation of these methods for measuring extractable B in 57 soil samples with pH values ranging from 5.3 to 9.5. There was a significant correlation between B values determined by the two methods, and the correlation coefficient was greater for soil samples with pH in the neutral to alkaline range. Interaction between soil samples and methods (ICP or colorimetric) was significant except for soil samples in the pH range of 8.0 to 9.5. Precision for B determination was greater with the ICP than with the colorimetric method

An exploration of dropout with RNNs for natural language inference.

Dropout is a crucial regularization technique for the Recurrent Neural Network (RNN) models of Natural Language Inference (NLI). However, dropout has not been evaluated for the effectiveness at different layers and dropout rates in NLI models. In this paper, we propose a novel RNN model for NLI and empirically evaluate the effect of applying dropout at different layers in the model. We also investigate the impact of varying dropout rates at these layers. Our empirical evaluation on a large (Stanford Natural Language Inference (SNLI)) and a small (SciTail) dataset suggest that dropout at each feed-forward connection severely affects the model accuracy at increasing dropout rates. We also show that regularizing the embedding layer is efficient for SNLI whereas regularizing the recurrent layer improves the accuracy for SciTail. Our model achieved an accuracy 86.14% on the SNLI dataset and 77.05% on SciTail

Surfactant protein D inhibits HIV-1 infection of target cells via interference with gp120-CD4 interaction and modulates pro-inflammatory cytokine production

© 2014 Pandit et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Surfactant Protein SP-D, a member of the collectin family, is a pattern recognition protein, secreted by mucosal epithelial cells and has an important role in innate immunity against various pathogens. In this study, we confirm that native human SP-D and a recombinant fragment of human SP-D (rhSP-D) bind to gp120 of HIV-1 and significantly inhibit viral replication in vitro in a calcium and dose-dependent manner. We show, for the first time, that SP-D and rhSP-D act as potent inhibitors of HIV-1 entry in to target cells and block the interaction between CD4 and gp120 in a dose-dependent manner. The rhSP-D-mediated inhibition of viral replication was examined using three clinical isolates of HIV-1 and three target cells: Jurkat T cells, U937 monocytic cells and PBMCs. HIV-1 induced cytokine storm in the three target cells was significantly suppressed by rhSP-D. Phosphorylation of key kinases p38, Erk1/2 and AKT, which contribute to HIV-1 induced immune activation, was significantly reduced in vitro in the presence of rhSP-D. Notably, anti-HIV-1 activity of rhSP-D was retained in the presence of biological fluids such as cervico-vaginal lavage and seminal plasma. Our study illustrates the multi-faceted role of human SPD against HIV-1 and potential of rhSP-D for immunotherapy to inhibit viral entry and immune activation in acute HIV infection. © 2014 Pandit et al.The work (Project no. 2011-16850) was supported by Medical Innovation Fund of Indian Council of Medical Research, New Delhi, India (www.icmr.nic.in/)