60 research outputs found
Relaxation and Simplex mathematical algorithms applied to the study of steady-state electrochemical responses of immobilized enzyme biosensors: Comparison with experiments
AbstractA description of the implementation of the relaxation method with automatic mesh point allocation for immobilized enzyme electrodes is presented. The advantages of this method for the solution of coupled reaction–diffusion problems are discussed. The relaxation numerical simulation technique is combined with the Simplex fitting algorithm to extract kinetic parameters from experimental data. The results of the simulations are compared to experimental data from self-assembled multilayered electrodes comprised of glucose oxidase (GOx) and an Os modified redox mediator and found to be in excellent agreement
Machine-Part cell formation through visual decipherable clustering of Self Organizing Map
Machine-part cell formation is used in cellular manufacturing in order to
process a large variety, quality, lower work in process levels, reducing
manufacturing lead-time and customer response time while retaining flexibility
for new products. This paper presents a new and novel approach for obtaining
machine cells and part families. In the cellular manufacturing the fundamental
problem is the formation of part families and machine cells. The present paper
deals with the Self Organising Map (SOM) method an unsupervised learning
algorithm in Artificial Intelligence, and has been used as a visually
decipherable clustering tool of machine-part cell formation. The objective of
the paper is to cluster the binary machine-part matrix through visually
decipherable cluster of SOM color-coding and labelling via the SOM map nodes in
such a way that the part families are processed in that machine cells. The
Umatrix, component plane, principal component projection, scatter plot and
histogram of SOM have been reported in the present work for the successful
visualization of the machine-part cell formation. Computational result with the
proposed algorithm on a set of group technology problems available in the
literature is also presented. The proposed SOM approach produced solutions with
a grouping efficacy that is at least as good as any results earlier reported in
the literature and improved the grouping efficacy for 70% of the problems and
found immensely useful to both industry practitioners and researchers.Comment: 18 pages,3 table, 4 figure
Microcellular Electrode Material for Microbial Bioelectrochemical Systems Synthesized by Hydrothermal Carbonization of Biomass Derived Precursors
V.F. acknowledges a UQ Postdoctoral Fellowship. This work was supported by the Australian Research Council Grant DP110100539. The authors acknowledge the facilities and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis (The University of Queensland). The Ghent University Special Research Fund (BOF) is acknowledged for the postdoctoral grant of M.N.B
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A computational study on outliers in world music
The comparative analysis of world music cultures has been the focus of several ethnomusicological studies in the last century. With the advances of Music Information Retrieval and the increased accessibility of sound archives, large-scale analysis of world music with computational tools is today feasible. We investigate music similarity in a corpus of 8200 recordings of folk and traditional music from 137 countries around the world. In particular, we aim to identify music recordings that are most distinct compared to the rest of our corpus. We refer to these recordings as ‘outliers’. We use signal processing tools to extract music information from audio recordings, data mining to quantify similarity and detect outliers, and spatial statistics to account for geographical correlation. Our findings suggest that Botswana is the country with the most distinct recordings in the corpus and China is the country with the most distinct recordings when considering spatial correlation. Our analysis includes a comparison of musical attributes and styles that contribute to the ‘uniqueness’ of the music of each country
The application of the relaxation and simplex method to the analysis of data for glucose electrodes based on glucose oxidase immobilised in an osmium redox polymer
Data for a series of fully integrated glucose oxidase, osmium redox polyelectrolyte layers deposited on thiolated gold electrodes by layer-by-layer self assembly was analysed using the relaxation and simplex method described in our earlier work (Flexer et al., 2008) [12]. The layer-by-layer assembly method allows fine control over the film thickness, enzyme loading, osmium and glucose concentrations with good reproducibility from electrode to electrode. In the analysis we combine the use of approximate analytical expressions with digital simulation to fit the data from an extensive set of experiments. The analysis shows a thickness dependence of the fraction of “wired enzyme molecules” and second order enzyme re-oxidation rate constant for thin films (below 300 nm) following changes in the multilayer film structure. For films thicker than 300 nm the kinetic data approach that of a redox hydrogel.<br/
Squatters. Castelo atalaya. Cartagena
Cartagena, Spai
Modelling Biosensor Responses
10.1002/9780470753842.ch8Bioelectrochemistry: Fundamentals, Experimental Techniques and Applications267-32
Design and synthesis of synthetic UP elements for modulation of gene expression in Escherichia coli
Metabolic engineering requires fine-tuned gene expression for most pathway optimization applications. To develop a suitable suite of promoters, traditional bacterial promoter engineering efforts have focused on modifications to the core region, especially the −10 and −35 regions, of native promoters. Here, we demonstrate an alternate, unexplored route of promoter engineering through randomization of the UP element of the promoter—a region that contacts the alpha subunit carboxy-terminal domain instead of the sigma subunit of the RNA polymerase holoenzyme. Through this work, we identify five novel UP element sequences through library-based searches in Escherichia coli. The resulting elements were used to activate the E. coli core promoter, rrnD promoter, to levels on par and higher than the prevalent strong bacterial promoter, OXB15. These relative levels of expression activation were transferrable when applied upstream of alternate core promoter sequences, including rrnA and rrnH. This work thus presents and validates a novel strategy for bacterial promoter engineering with transferability across varying core promoters and potential for transferability across bacterial species. Keywords: UP element, Promoter engineering, Expression modulatio
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