Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803

Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental conditions. In this protocol, we describe a procedure for the isolation of 70S ribosomes from the unicellular cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). We have successfully used this protocol in our study of the cyanobacterial ribosomal-associated protein LrtA, which is a homologue of bacterial HPF (hibernation promoting factor) (Galmozzi et al., 2016).España, Junta de Andalucía grant P07-CVI-02792 and group BIO-284España, MINECO y Fondo Social Europeo grant BFU2013-41712-

Consensus in multi-agent systems with non-periodic sampled-data exchange and uncertain network topology

In this paper consensus in second-order multi-agent systems with a non-periodic sampled-data exchange among agents is investigated. The sampling is random with bounded inter-sampling intervals. It is assumed that each agent has exact knowledge of its own state at any time instant. The considered local interaction rule is PD-type. Sufficient conditions for stability of the consensus protocol to a time-invariant value are derived based on LMIs. Such conditions only require the knowledge of the connectivity of the graph modeling the network topology. Numerical simulations are presented to corroborate the theoretical results.Comment: arXiv admin note: substantial text overlap with arXiv:1407.300

Consensus in multi-agent systems with second-order dynamics and non-periodic sampled-data exchange

In this paper consensus in second-order multi-agent systems with a non-periodic sampled-data exchange among agents is investigated. The sampling is random with bounded inter-sampling intervals. It is assumed that each agent has exact knowledge of its own state at all times. The considered local interaction rule is PD-type. The characterization of the convergence properties exploits a Lyapunov-Krasovskii functional method, sufficient conditions for stability of the consensus protocol to a time-invariant value are derived. Numerical simulations are presented to corroborate the theoretical results.Comment: The 19th IEEE International Conference on Emerging Technologies and Factory Automation (ETFA'2014), Barcelona (Spain

VLT spectroscopy of globular clusters in the Sombrero galaxy

We have obtained intermediate-resolution VLT spectroscopy of 75 globular cluster candidates around the Sa galaxy M104 (NGC4594). Fifty-seven candidates out to ~ 40 kpc in the halo of the galaxy were confirmed to be bona-fide globular clusters, 27 of which are new. A first analysis of the velocities provides only marginal evidence for rotation of the cluster system. From Hbeta line strengths, almost all of the clusters in our sample have ages that are consistent, within the errors, with Milky Way globular clusters. Only a few clusters may be 1-2 Gyr old, and bulge and halo clusters appear coeval. The absorption line indices follow the correlations established for the Milky Way clusters. Metallicities are derived based upon new empirical calibrations with Galactic globular clusters taking into account the non-linear behavior of some indices (e.g., Mg2). Our sample of globular clusters in NGC4594 spans a metallicity range of -2.13 < [Fe/H] < +0.26 dex, and the median metallicity of the system is [Fe/H] = -0.85. Thus, our data provide evidence that some of the clusters have super-solar metallicity. Overall, the abundance distribution of the cluster system is consistent with a bimodal distribution with peaks at [Fe/H] ~- 1.7 and -0.7. However, the radial change in the metallicity distribution of clusters may not be straightforwardly explained by a varying mixture of two sub-populations of red and blue clusters.Comment: 6 pages (incl. 4 figures) to appear in the proceedings of the ESO workshop "Extragalactic Globular Cluster Systems", ESO Astrophysics Symp., Garching bei Muenchen (Germany), ed. Kissler-Patig M., Springer-Verlag: Heidelber

Cognitive and affective components of challenge and threat states

We explored the cognitive and affective components of the Theory of Challenge and Threat States in Athletes (TCTSA) using a cross-sectional design. One hundred and seventy-seven collegiate athletes indicated how they typically approached an important competition on measures of self-efficacy, perceived control, achievement goals, emotional states and interpretation of emotional states. Participants also indicated to what extent they typically perceived the important competition as a challenge and/or a threat. The results suggest that a perception of challenge was not predicted by any of the cognitive components. A perception of threat was positively predicted by avoidance goals and negatively predicted by self-efficacy and approach goals. Both challenge and threat had a positive relationship with anxiety. Practical implications of this study are that an avoidance orientation appeared to be related to potentially negative constructs such as anxiety, threat and dejection. The findings may suggest that practitioners and researchers should focus on reducing an avoidance orientation, however the results should be treated with caution in applied settings, as this study did not examine how the combination of constructs exactly influences sport performance. The results provided partial support for the TCTSA with stronger support for proposed relationships with threat rather than challenge states

The effects of youth training on risk behavior: the role of non-cognitive skills

This paper uses unique experimental data from a youth training program in the Favelas, Brazil, to examine whether youth training programs can prevent treatment recipients from engaging in risk behavior-i.e., cigarettes, alcohol, and hard drug utilization, as well as witnessing or being a victim of violent crime. Although the program was successful in increasing income, we find that it only improved risk behavior of the treated individuals with higher levels of socio-emotional skills

Size reconstructibility of graphs

The deck of a graph $G$ is given by the multiset of (unlabelled) subgraphs $\{G-v:v\in V(G)\}$. The subgraphs $G-v$ are referred to as the cards of $G$. Brown and Fenner recently showed that, for $n\geq29$, the number of edges of a graph $G$ can be computed from any deck missing 2 cards. We show that, for sufficiently large $n$, the number of edges can be computed from any deck missing at most $\frac1{20}\sqrt{n}$ cards.Comment: 15 page