299 research outputs found
Hospital use of young children in Switzerland: A nation-wide study based on a complete survey over 4 years
<p>Abstract</p> <p>Background</p> <p>Young children are known to be the most frequent hospital users compared to older children and young adults. Therefore, they are an important population from economic and policy perspectives of health care delivery. In Switzerland complete hospitalization discharge records for children [<5 years] of four consecutive years [2002–2005] were evaluated in order to analyze variation in patterns of hospital use.</p> <p>Methods</p> <p>Stationary and outpatient hospitalization rates on aggregated ZIP code level were calculated based on census data provided by the Swiss federal statistical office (BfS). Thirty-seven hospital service areas for children [HSAP] were created with the method of "small area analysis", reflecting user-based health markets. Descriptive statistics and general linear models were applied to analyze the data.</p> <p>Results</p> <p>The mean stationary hospitalization rate over four years was 66.1 discharges per 1000 children. Hospitalizations for respiratory problem are most dominant in young children (25.9%) and highest hospitalization rates are associated with geographical factors of urban areas and specific language regions. Statistical models yielded significant effect estimates for these factors and a significant association between ambulatory/outpatient and stationary hospitalization rates.</p> <p>Conclusion</p> <p>The utilization-based approach, using HSAP as spatial representation of user-based health markets, is a valid instrument and allows assessing the supply and demand of children's health care services. The study provides for the first time estimates for several factors associated with the large variation in the utilization and provision of paediatric health care resources in Switzerland.</p
Planetary evolution with atmospheric photoevaporation II: Fitting the slope of the radius valley by combining boil-off and XUV-driven escape
The Kepler satellite has revealed a gap between sub-Neptunes and super-Earths
that atmospheric escape models had predicted as an evaporation valley. We seek
to contrast results from a simple XUV-driven energy-limited (ELIM) escape model
against those from a direct hydrodynamic (HYDRO) model. Besides XUV-driven
escape, the latter also includes the boil-off regime. We couple the two models
to an internal structure model and follow the planets' temporal evolution over
Gyr. To see the population-wide imprint of the two models, we first employ a
rectangular grid in initial conditions. We then study the slope of the valley
also for initial conditions derived from the Kepler planets. For the
rectangular grid, we find that the power-law slope of the valley with respect
to orbital period is -0.18 and -0.11 in the ELIM and HYDRO model, respectively.
For the initial conditions derived from the Kepler planets, the results are
similar (-0.16 and -0.10). While the slope found with the ELIM model is steeper
than observed, the one of the HYDRO model is in excellent agreement with
observations. The reason for the shallower slope is caused by the two regimes
in which the ELIM model fails: First, puffy planets at low stellar irradiation.
For them, boil-off dominates mass loss. However, boil-off is absent in the ELIM
model, thus it underestimates escape relative to HYDRO. Second, massive compact
planets at high XUV irradiation. For them, the ELIM approximation overestimates
escape relative to the HYDRO case because of cooling by thermal conduction,
neglected in the ELIM model. The two effects act together in concert to yield
in the HYDRO model a shallower slope of the valley that agrees very well with
observations. We conclude that an escape model that includes boil-off and a
more realistic treatment of cooling mechanisms can reproduce one of the most
important constraints, the valley slope.Comment: 20 pages, 11 figures, accepted to A&
Author Correction: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc
Author Correction: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc
The mating-specific Gα interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast
As a budding yeast cell elongates toward its mating partner, cytoplasmic microtubules connect the nucleus to the cell cortex at the growth tip. The Kar3 kinesin-like motor protein is then thought to stimulate plus-end depolymerization of these microtubules, thus drawing the nucleus closer to the site where cell fusion and karyogamy will occur. Here, we show that pheromone stimulates a microtubule-independent interaction between Kar3 and the mating-specific Gα protein Gpa1 and that Gpa1 affects both microtubule orientation and cortical contact. The membrane localization of Gpa1 was found to polarize early in the mating response, at about the same time that the microtubules begin to attach to the incipient growth site. In the absence of Gpa1, microtubules lose contact with the cortex upon shrinking and Kar3 is improperly localized, suggesting that Gpa1 is a cortical anchor for Kar3. We infer that Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating. © 2009 by The American Society for Cell Biology
Mechanical Stress Inference for Two Dimensional Cell Arrays
Many morphogenetic processes involve mechanical rearrangement of epithelial
tissues that is driven by precisely regulated cytoskeletal forces and cell
adhesion. The mechanical state of the cell and intercellular adhesion are not
only the targets of regulation, but are themselves likely signals that
coordinate developmental process. Yet, because it is difficult to directly
measure mechanical stress {\it in vivo} on sub-cellular scale, little is
understood about the role of mechanics of development. Here we present an
alternative approach which takes advantage of the recent progress in live
imaging of morphogenetic processes and uses computational analysis of high
resolution images of epithelial tissues to infer relative magnitude of forces
acting within and between cells. We model intracellular stress in terms of bulk
pressure and interfacial tension, allowing these parameters to vary from cell
to cell and from interface to interface. Assuming that epithelial cell layers
are close to mechanical equilibrium, we use the observed geometry of the two
dimensional cell array to infer interfacial tensions and intracellular
pressures. Here we present the mathematical formulation of the proposed
Mechanical Inverse method and apply it to the analysis of epithelial cell
layers observed at the onset of ventral furrow formation in the {\it
Drosophila} embryo and in the process of hair-cell determination in the avian
cochlea. The analysis reveals mechanical anisotropy in the former process and
mechanical heterogeneity, correlated with cell differentiation, in the latter
process. The method opens a way for quantitative and detailed experimental
tests of models of cell and tissue mechanics
ESPRESSO: The next European exoplanet hunter
The acronym ESPRESSO stems for Echelle SPectrograph for Rocky Exoplanets and
Stable Spectroscopic Observations; this instrument will be the next VLT high
resolution spectrograph. The spectrograph will be installed at the
Combined-Coud\'e Laboratory of the VLT and linked to the four 8.2 m Unit
Telescopes (UT) through four optical Coud\'e trains. ESPRESSO will combine
efficiency and extreme spectroscopic precision. ESPRESSO is foreseen to achieve
a gain of two magnitudes with respect to its predecessor HARPS, and to improve
the instrumental radial-velocity precision to reach the 10 cm/s level. It can
be operated either with a single UT or with up to four UTs, enabling an
additional gain in the latter mode. The incoherent combination of four
telescopes and the extreme precision requirements called for many innovative
design solutions while ensuring the technical heritage of the successful HARPS
experience. ESPRESSO will allow to explore new frontiers in most domains of
astrophysics that require precision and sensitivity. The main scientific
drivers are the search and characterization of rocky exoplanets in the
habitable zone of quiet, nearby G to M-dwarfs and the analysis of the
variability of fundamental physical constants. The project passed the final
design review in May 2013 and entered the manufacturing phase. ESPRESSO will be
installed at the Paranal Observatory in 2016 and its operation is planned to
start by the end of the same year.Comment: 12 pages, figures included, accepted for publication in Astron. Nach
Branch Mode Selection during Early Lung Development
Many organs of higher organisms, such as the vascular system, lung, kidney,
pancreas, liver and glands, are heavily branched structures. The branching
process during lung development has been studied in great detail and is
remarkably stereotyped. The branched tree is generated by the sequential,
non-random use of three geometrically simple modes of branching (domain
branching, planar and orthogonal bifurcation). While many regulatory components
and local interactions have been defined an integrated understanding of the
regulatory network that controls the branching process is lacking. We have
developed a deterministic, spatio-temporal differential-equation based model of
the core signaling network that governs lung branching morphogenesis. The model
focuses on the two key signaling factors that have been identified in
experiments, fibroblast growth factor (FGF10) and sonic hedgehog (SHH) as well
as the SHH receptor patched (Ptc). We show that the reported biochemical
interactions give rise to a Schnakenberg-type Turing patterning mechanisms that
allows us to reproduce experimental observations in wildtype and mutant mice.
The kinetic parameters as well as the domain shape are based on experimental
data where available. The developed model is robust to small absolute and large
relative changes in the parameter values. At the same time there is a strong
regulatory potential in that the switching between branching modes can be
achieved by targeted changes in the parameter values. We note that the sequence
of different branching events may also be the result of different growth
speeds: fast growth triggers lateral branching while slow growth favours
bifurcations in our model. We conclude that the FGF10-SHH-Ptc1 module is
sufficient to generate pattern that correspond to the observed branching modesComment: Initially published at PLoS Comput Bio
Generation of an equine biobank to be used for Functional Annotation of Animal Genomes project
The Functional Annotation of Animal Genomes (FAANG) project aims to identify genomic regulatory elements in both sexes across multiple stages of development in domesticated animals. This study represents the first stage of the FAANG project for the horse, Equus caballus. A biobank of 80 tissue samples, two cell lines and six body fluids was created from two adult Thoroughbred mares. Ante-mortem assessments included full physical examinations, lameness, ophthalmologic and neurologic evaluations. Complete blood counts and serum biochemistries were also performed. At necropsy, in addition to tissue samples, aliquots of serum, ethylenediaminetetraacetic acid (EDTA) plasma, heparinized plasma, cerebrospinal fluid, synovial fluid, urine and microbiome samples from all regions of the gastrointestinal and urogenital tracts were collected. Epidermal keratinocytes and dermal fibroblasts were cultured from skin samples. All tissues were grossly and histologically evaluated by a board-certified veterinary pathologist. The results of the clinical and pathological evaluations identified subclinical eosinophilic and lymphocytic infiltration throughout the length of the gastrointestinal tract as well as a mild clinical lameness in both animals. Each sample was cryo-preserved in multiple ways, and nuclei were extracted from selected tissues. These samples represent the first published systemically healthy equine-specific biobank with extensive clinical phenotyping ante- and post-mortem. The tissues in the biobank are intended for community-wide use in the functional annotation of the equine genome. The use of the biobank will improve the quality of the reference annotation and allow all equine researchers to elucidate unknown genomic and epigenomic causes of disease
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