69 research outputs found
Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)
13C-direct detected NMR experiments for the sequential J-based resonance assignment of RNA oligonucleotides
We present here a set of 13C-direct detected NMR experiments to facilitate the resonance assignment of RNA oligonucleotides. Three experiments have been developed: (1) the (H)CC-TOCSY-experiment utilizing a virtual decoupling scheme to assign the intraresidual ribose 13C-spins, (2) the (H)CPC-experiment that correlates each phosphorus with the C4′ nuclei of adjacent nucleotides via J(C,P) couplings and (3) the (H)CPC-CCH-TOCSY-experiment that correlates the phosphorus nuclei with the respective C1′,H1′ ribose signals. The experiments were applied to two RNA hairpin structures. The current set of 13C-direct detected experiments allows direct and unambiguous assignment of the majority of the hetero nuclei and the identification of the individual ribose moieties following their sequential assignment. Thus, 13C-direct detected NMR methods constitute useful complements to the conventional 1H-detected approach for the resonance assignment of oligonucleotides that is often hindered by the limited chemical shift dispersion. The developed methods can also be applied to large deuterated RNAs
Allosteric effects in cyclophilin mutants may be explained by changes in nano-microsecond time scale motions
The relationship between molecular motion and catalysis in enzymes is debated. Here, simulations of cyclophilin A and three catalytically-impaired mutants reveal a nanosecond-scale interconversion between active and inactive conformations, orders of magnitude faster than previously suggested
Intensitätsmodulierte Radiotherapie des Prostatakarzinoms mit simultanem integriertem Boost nach molekularer Bildgebung mit 18F-Cholin-PET-CT - klinische Ergebnisse und Lebensqualität
Intensitätsmodulierte Radiotherapie des Prostatakarzinoms mit simultanem integriertem Boost nach molekularer Bildgebung mit 18F-Cholin-PET-CT - klinische Ergebnisse und Lebensqualität
Antibiotika aus Basidiomyceten, XX Synthese von Strobilurin A und Revision der Stereochemie der natürlichen Strobilurine
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