Buruli Ulcer in Long-Term Traveler to Senegal

A resource usage view consists of models that are important assets to analyze and control usage of hardware resources such as processors and memory elements. In this paper, we present the application of a top-down approach to construct a resource usage view using actual system runtime information. The models in this view help to address the concerns of a resource usage viewpoint providing information in terms of system-specific architecture and design execution elements such as execution scenarios, tasks, software components, processes, and threads. As part of our validation, constructing a resource usage view of an MRI system, a representative large software-intensive system, we observed that the constructed models for this view provide useful overviews and insights for practitioners to describe part of the system execution architecture and especially to analyze and evaluate the use of hardware resources in a top-down fashion.

Evaluation of an immunomagnetic separation method to capture Candida yeasts cells in blood

BackgroundCandida species have become the fourth most-frequent cause of nosocomial bloodstream infections in immunocompromised patients. Therefore, rapid identification of pathogenic fungi to species level has been considered critical for treatment. Conventional diagnostic procedures such as blood culture or biochemical tests are lacking both sensitivity and species specificity, so development of rapid diagnostic is essential. Results An immunomagnetic method involving anti-Candida monoclonal antibodies was developed to capture and concentrate in human blood four different species of Candida cells responsible for invasive yeast infections. In comparison with an automated blood culture, processing time of immunomagnetic separation is shorter, saving at least 24 hours to obtain colonies before identification. Conclusion Thus, this easy to use method provides a promising basis for concentrating all Candida species in blood to improve sensitivity before identification

Buruli Ulcer in Long-Term Traveler to Senegal

International audienc

Induction of Heme Oxygenase-1, Biliverdin Reductase and H-Ferritin in Lung Macrophage in Smokers with Primary Spontaneous Pneumothorax: Role of HIF-1α

Few data concern the pathophysiology of primary spontaneous pneumothorax (PSP), which is associated with alveolar hypoxia/reoxygenation. This study tested the hypothesis that PSP is associated with oxidative stress in lung macrophages. We analysed expression of the oxidative stress marker 4-HNE; the antioxidant and anti-inflammatory proteins heme oxygenase-1 (HO-1), biliverdin reductase (BVR) and heavy chain of ferritin (H-ferritin); and the transcription factors controlling their expression Nrf2 and HIF-1alpha, in lung samples from smoker and nonsmoker patients with PSP (PSP-S and PSP-NS), cigarette smoke being a risk factor of recurrence of the disease.mRNA was assessed by RT-PCR and proteins by western blot, immunohistochemistry and confocal laser analysis. 4-HNE, HO-1, BVR and H-ferritin were increased in macrophages from PSP-S as compared to PSP-NS and controls (C). HO-1 increase was associated with increased expression of HIF-1alpha mRNA and protein in alveolar macrophages in PSP-S patients, whereas Nrf2 was not modified. To understand the regulation of HO-1, BVR and H-ferritin, THP-1 macrophages were exposed to conditions mimicking conditions in C, PSP-S and PSP-NS patients: cigarette smoke condensate (CS) or air exposure followed or not by hypoxia/reoxygenation. Silencing RNA experiments confirmed that HIF-1alpha nuclear translocation was responsible for HO-1, BVR and H-ferritin induction mediated by CS and hypoxia/reoxygenation.PSP in smokers is associated with lung macrophage oxidative stress. The response to this condition involves HIF-1alpha-mediated induction of HO-1, BVR and H-ferritin

Identification de gènes de candida albicans potentionnellement impliqués lors des phénomènes d'adhérence aux surfaces inertes

ANGERS-BU Médecine-Pharmacie (490072105) / SudocSudocFranceF

Effect of a 24-h continuous walking race on cardiac autonomic control.

This study investigated the relationships between walking speed and heart rate (HR) variability (HRV) in eleven subjects during a 24-h race. It was hypothesized that the nycthemeral rhythm on HR is preserved during the race. RR intervals and walking speed were measured. Fast Fourier transform was applied to samples of 1,024 successive RR intervals collected every hour from a HR monitor. Walking speed was averaged every hour and decreased (first lap: 8.8 +/- 0.3 vs. last lap: 7.3 +/- 0.8 km h(-1), P < 0.001) with HR also decreasing (max at 19:00 h: 143 +/- 9 vs. min at 7:00 h: 117 +/- 14 beats min(-1), P < 0.001) following a third order polynomial shape. HRV power spectral components followed distribution patterns similar to the mean RR during the race with a minimum in the early evening (19:00-20:00 h) and a maximum in the morning (5:00-8:00 h). Thus, as for mean RR, spectral components over time are also fitted to a third order polynomial regression. LF/HF ratio increased linearly (min = 0.5 +/- 0.3, max = 2.8 +/- 5.3, P = 0.02). Although mean HF peak did not decrease significantly over time, it was positively correlated with walking speed. In conclusion, this study showed that despite a constant decrease in walking speed, HR circadian rhythm is preserved during a continuous 24-h walking race. The short-term HRV components remain linked to HR whereas the LF/HF ratio increases linearly until the end of the race whatever HR is

Effect of a 24-h continuous walking race on cardiac autonomic control

This study investigated the relationships between walking speed and heart rate (HR) variability (HRV) in eleven subjects during a 24-h race. It was hypothesized that the nycthemeral rhythm on HR is preserved during the race. RR intervals and walking speed were measured. Fast Fourier transform was applied to samples of 1,024 successive RR intervals collected every hour from a HR monitor. Walking speed was averaged every hour and decreased (first lap: 8.8 +/- 0.3 vs. last lap: 7.3 +/- 0.8 km h(-1), P < 0.001) with HR also decreasing (max at 19:00 h: 143 +/- 9 vs. min at 7:00 h: 117 +/- 14 beats min(-1), P < 0.001) following a third order polynomial shape. HRV power spectral components followed distribution patterns similar to the mean RR during the race with a minimum in the early evening (19:00-20:00 h) and a maximum in the morning (5:00-8:00 h). Thus, as for mean RR, spectral components over time are also fitted to a third order polynomial regression. LF/HF ratio increased linearly (min = 0.5 +/- 0.3, max = 2.8 +/- 5.3, P = 0.02). Although mean HF peak did not decrease significantly over time, it was positively correlated with walking speed. In conclusion, this study showed that despite a constant decrease in walking speed, HR circadian rhythm is preserved during a continuous 24-h walking race. The short-term HRV components remain linked to HR whereas the LF/HF ratio increases linearly until the end of the race whatever HR is