Caveolin-1 Modulates Mechanotransduction Responses to Substrate Stiffness through Actin-Dependent Control of YAP

The transcriptional regulator YAP orchestrates many cellular functions, including tissue homeostasis, organ growth control, and tumorigenesis. Mechanical stimuli are a key input to YAP activity, but the mechanisms controlling this regulation remain largely uncharacterized. We show that CAV1 positively modulates the YAP mechanoresponse to substrate stiffness through actin-cytoskeleton-dependent and Hippo-kinase-independent mechanisms. RHO activity is necessary, but not sufficient, for CAV1-dependent mechanoregulation of YAP activity. Systematic quantitative interactomic studies and image-based small interfering RNA (siRNA) screens provide evidence that this actin-dependent regulation is determined by YAP interaction with the 14-3-3 protein YWHAH. Constitutive YAP activation rescued phenotypes associated with CAV1 loss, including defective extracellular matrix (ECM) remodeling. CAV1-mediated control of YAP activity was validated in vivo in a model of pancreatitis-driven acinar-to-ductal metaplasia. We propose that this CAV1-YAP mechanotransduction system controls a significant share of cell programs linked to these two pivotal regulators, with potentially broad physiological and pathological implications. Moreno-Vicente et al. report that CAV1, a key component of PM mechanosensing caveolae, mediates adaptation to ECM rigidity by modulating YAP activity through the control of actin dynamics and phosphorylation-dependent interaction of YAP with the 14-3-3-domain protein YWHAH. Cav1-dependent YAP regulation drives two pathophysiological processes: ECM remodeling and pancreatic ADM. © 2018 The Author

Controlling eutrophication by means of water recirculation: an optimal control perspective

In this work, the artificial recirculation of water is presented and analyzed, from the perspective of the optimal control of partial differential equations, as a tool to prevent eutrophication effects in large waterbodies. A novel formulation of the environmental problem, based on the coupling of nonlinear models for hydrodynamics, water temperature and concentrations of the different species involved in the eutrophication processes, is introduced. After a complete and rigorous analysis of the existence of optimal solutions, a full numerical algorithm for their computation is proposed. Finally, some numerical results for a realistic scenario are shown, in order to prove the efficiency of our approach.Xunta de Galicia | Ref. ED431C 2019/02Agencia Estatal de Investigación | Ref. MTM2016-75140-

Multilevel Multiphase Feedforward Space-Vector Modulation Technique

Multiphase converters have been applied to an increasing number of industrial applications in recent years. On the other hand, multilevel converters have become a mature technology mainly in medium- and high-power applications. One of the problems of multilevel converters is the dc voltage unbalance of the dc bus. Depending on the loading conditions and the number of levels of the converter, oscillations appear in the dc voltages of the dc link. This paper presents a feedforward modulation technique for multilevel multiphase converters that reduces the distortion under balanced or unbalanced dc conditions. The proposed modulation method can be applied to any multilevel-converter topology with any number of levels and phases. Experimental results are shown in order to validate the proposed feedforward modulation technique.Ministerio de Ciencia e Innovación DPI2009-07004Ministerio de Eduación y Ciencia TEC2007-6187

Extrusión del componente plástico de la copa acetabular sin luxación protésica: a propósito de un caso

Se present a un caso de extrusión del component e plástic o (polietileno) ocurrido en el transcurso del primer me s del postoperatorio en un pacient e que había sufrido un recambio del component e acetabular. Se destaca el hecho de que cursase de forma asintomática y de que no se acompañase de luxación de la prótesis.A case of disassembly of the polyethylene component of the acetabular cup acurred in a patient one mouth after revision surgery for loosening of a screwed acetabular cup is reported. Special emphasis is made in the asyntomati c clinic and on the no association with dislocation of the femoral component

Position Control of a Quadrotor under External Constant Disturbance

International audienceIn the present work, an adaptive backstepping algorithm is developed in order to counteract the effects of disturbances. These disturbances are modeled as a constant force in the translational model part and as a constant torque in the orientation model part. We make the deduction of the mathematical expression for the proposed control algorithm and also we show its performance in simulation. Additionally, we include some experiments for validating the results obtained via simulation

Evaluación del área trabecular por digitalización de imágenes en el callo de fractura: estudio experimental

Se presenta un método de evaluación del área trabecular por medio de digitalización de imágenes de microscopía óptica. Se estudia la evolución de 20 callos de fractura en ratas de cepa Wistar, obteniendo un incremento progresivo del área trabecular desde los 14 a los 49 días, correlacionando ésta directamente con la actividad neoformativa. Se concluye proponiendo el uso de este método por su uniformidad, objetividad y reproductibilidad en todo el estudio, permitiendo la independencia del anatomopatológico.We present a method for assessment of the bone trabecular area by using optic microscope digitalized imaging. We studied 20 fracture callus performed at the tibia in Wistar rats. The bone trabecular area had a progressive increased from 14 to 49 days, post-fracture, been directly related with the neoformative activity. We propose this method because of this uniformity, objetivity and reliability. This method akows fracture repair analysis independently of the subjetivity of the pathologist

Of Fears and Budgets: Strategies of Control in Vespa velutina Invasion and Lessons for Best Management Practices

[Abstrat] Implementing management practices for the control of invasive species can be a complex task with multiple dimensions, where the identification of stakeholders and drivers of those practices is of paramount importance. The invasive hornet Vespa velutina has spread across Europe and Asia from its native range in SE Asia in recent years. A common control method is the removal and destruction of its nests on citizens’ request to call centers. In this paper we have explored the knowledge and main factors that influence the perceptions of the citizens on the species in an invaded municipality in NW Spain, as well as the management practices of the municipal emergency unit responsible for nest removal activities. Our analysis brings out multiple drivers of management practices that derive both from the citizens’ and practitioners’ knowledge, and highlights several points of conflict between both stakeholder groups connected to (1) the degree of service provided to the local population, (2) the risk of allergic reactions as a motive to urge removals, or (3) the quality of information provided by mass media. Our results support the crucial importance of environmental education programs that seek to increase the knowledge of the general public about the threats of invasive species. Such programs might be incorporated to implement and optimize management plans of V. velutina by enhancing communication between experts and local population