Anti-inflammatory and analgesic propertiesof SS-68 indole derivative

The experiments with rats show that when administered intravenously (5 mg/kg) and intragastrically (5, 10, 15 and 20 mg/kg), SS-68 compound exerts an antiinflammatory effect (AIE) on models of acute inflammation, caused by carrageenin and serotonin, and exerts no AIE in case of edema induced by complete Freund‘s adjuvant (CFA) and histamine, except for intragastric administration at a dose of 20 mg/kg. By its antiphlogogenic effect in case of carrageenin and serotonin edemas, SS-68 is comparable to diclofenac (5, 10 and 15 mg/kg intragastrically) and indomethacin (10 mg kg intragastriccally), and exceeds piroxicam (20 mg/kg intragastrically). By reference to its therapeutic index (TI), in the first case SS-68 exceeds diclofenac, indomethacin and piroxicam 2.2, 15.6 and 5.6 times, and in the second case it exceeds them 1.8, 12.8 and 4.4 times, respectivel

Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology

Genetic knowledge of microorganisms plays a critical role in the creation of new biotechnologies, since the effectiveness of any biotechnology is determined by the particular qualities of the structurally functional organization of molecular-genetic systems and their components used for the production of targeted products. Collections of microbial cultures play a decisive role in mobilizing biological resources and make it possible to form a solid base for genetic, molecular biological and biotechnological research. The aim of this work was to assess the key molecular-genetic and phenotypic characteristics of strains of the collection of microorganisms created in the “FRC Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences” as a genetic resource for biotechnology. Thirty strains of microorganisms of the collection were isolated by employees of the FRC ICG SB RAS from extreme natural ecosystems, the key molecular-genetic and phenotypic characteristics were described using modern methods of molecular biology and mass-spectrometry. DNA isolation and the sequencing of 16S rRNA gene sequences were performed. The strains of the collection were characterized by morphological, physiological, moleculargenetic and mass-spectrometric characteristics. The particular qualities of growing of strains on different substrates have been established, the study of cell morphology has been carried out. The physiological characteristics of the strains of the collection have been established: the attitude to oxygen, the type of nutrition, the range of temperature and pH, the attitude to NaCl and others. Different resistance of strains to antibiotics has been established. The creation of personal mass spectra of protein profiles of the studied strains of the collection was carried out. The resulting DNA sequences of the strains are deposited in the GenBank. The chemotaxonomic characteristics of strains have been determined. The biotechnological properties of the strains were assessed, the amount of metabolites (ethanol, lactic and acetic acids) in the culture liquid was determined. The value of the collection of microorganisms of the FRC ICG SB RAS as a genetic resource for biotechnology and bioengineering is determined not only by the species diversity of its strains, but also by a wide range of their area isolation and by the depth of their characterization using the widest arsenal of both classical and modern methods (including methods of genomics, proteomics, transcriptomics and bioinformatics)

An integrated method for taxonomic identif ication of microorganisms

For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. The aim of this work was to identify microorganisms from the ICG SB RAS Collection using an integrated approach that involves a combination of various phenotypic and genotypic characteristics. Key molecular-genetic and phenotypic characteristics were determined for 93 microbial strains from the ICG SB RAS Collection. The strains were characterized by means of morphological, physiological, moleculargenetic, and mass-spectrometric parameters. Specific features of the growth of the strains on different media were determined, and cell morphology was evaluated. The strains were tested for the ability to utilize various substrates. The strains studied were found to significantly differ in their biochemical characteristics. Physiological characteristics of the strains from the collection were identified too, e. g., the relationship with oxygen, type of nutrition, suitable temperature and pH ranges, and NaCl tolerance. In this work, the microorganisms analyzed were combined into separate groups based on the similarities of their phenotypic characteristics. This categorization, after further refinement and expansion of the spectrum of taxa and their metabolic maps, may serve as the basis for the creation of an “artificial” classification that can be used as a key for simplified and quicker identification and recognition of microorganisms within both the ICG SB RAS Collection and other collections

Candidal vulvovaginitis in girls: clotrimazole as the drug of choice

The article presents the results of a study on the effectiveness of using clotrimazole 2% to treat candidal vulvovaginitis in girls. The use of a clotrimazole 2% cream treatment regimen, (100 mg), intravaginally once daily in girls with candidal vulvovaginitis is justified and highly effective. The absence of side effects while taking clotrimazole increases the commitment to this type of therapy

H₂SO₄–H₂O–NH₃ ternary ion-mediated nucleation (TIMN): kinetic-based model and comparison with CLOUD measurements

New particle formation (NPF) is known to be an important source of atmospheric particles that impacts air quality, hydrological cycle, and climate. Although laboratory measurements indicate that ammonia enhances NPF, the physicochemical processes underlying the observed effect of ammonia on NPF are yet to be understood. Here we present a comprehensive kinetically based H2SO4–H2O–NH3 ternary ion-mediated nucleation (TIMN) model that is based on the thermodynamic data derived from both quantum-chemical calculations and laboratory measurements. NH3 was found to reduce nucleation barriers for neutral, positively charged, and negatively charged clusters differently, due to large differences in the binding strength of NH3, H2O, and H2SO4 to small clusters of different charging states. The model reveals the general favor of nucleation of negative ions, followed by nucleation on positive ions and neutral nucleation, for which higher NH3 concentrations are needed, in excellent agreement with Cosmics Leaving OUtdoor Droplets (CLOUD) measurements. The TIMN model explicitly resolves dependences of nucleation rates on all the key controlling parameters and captures the absolute values of nucleation rates as well as the dependence of TIMN rates on concentrations of NH3 and H2SO4, ionization rates, temperature, and relative humidity observed in the well-controlled CLOUD measurements well. The kinetic model offers physicochemical insights into the ternary nucleation process and provides a physics-based approach to calculate TIMN rates under a wide range of atmospheric conditions.</p

Structural and functional features of reparative osteogenesis under the influence of natural healing factors

Pavlenko K. V., Nasibullin B. A., Uvarova Ye. B., Gozhenko A. I. Структурно-функциональные особенности репаративного остеогенеза под влиянием природных лечебных факторов = Structural and functional features of reparative osteogenesis under the influence of natural healing factors. Journal of Education, Health and Sport. 2015;5(3):279-288. ISSN 2391-8306. DOI: 10.5281/zenodo.16453 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%283%29%3A279-288 https://pbn.nauka.gov.pl/works/551960 http://dx.doi.org/10.5281/zenodo.16453 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011 – 2014 http://journal.rsw.edu.pl/index.php/JHS/issue/archive   Deklaracja. Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie. Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r. The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014). © The Author (s) 2015; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 20.01.2014. Revised 27.02.2015. Accepted: 12.03.2015.   УДК 616.718.6-001.5-085.814.5   Структурно-функциональные особенности репаративного остеогенеза под влиянием природных лечебных факторов   Structural and functional features of reparative osteogenesis under the influence of natural healing factors   К.В. Павленко, Б.А. Насибуллин, Е.Б. Уварова, А.И. Гоженко K.V. Pavlenko, B.A. Nasibullin, Ye.B. Uvarova, A.I.Gozhenko   Городская клиническая больница № 11, Одесса Украинский НИИ медицины транспорта, Одесса Городская клиническая больница № 1, Одесса   City Clinical Hospital № 11, Odessa Ukrainian Research Institute for Medicine of Transport, Odessa City Clinical Hospital № 1, Odessa   Article On 92 white rats - male outbred breeding studied the effect on the healing process of fracture of fibula, as well as ion exchange Ca²+, phosphorus, and alkaline phosphatase activity in blood plasma. The process of fracture healing was similar to the interim indices in rats groups II and III, which was introduced at the fracture site platelet-rich plasma and performed phonophoresis with a fixed dose of poison. By the nature of the formation of callus, they differed. Therefore, in groups I and III process occurred on osteogenic type, and in group II on chondrogenic. In applying the platelet-rich plasma activated alkaline phosphatase decreased plasma calcium and phosphorus content in it. Under the influence of the activation proceeds phonophoresisCa²+ in the fracture, but unlike the II-group, this process is punctuated character. Suggested that the enriched plasma and phonophoresis with "Apizartron" promotes greater intake of calcium in the fracture, and promotes the activation of repair processes in the population of osteocytes. Keywords: fracture of the fibula; platelet-rich plasma; calcium metabolism; alkaline phosphatase; phonophoresis.               Реферат На 92 белых крысах – самцах аутобредного разведения изучали влияние на процесс заживления перелома малой берцовой кости, а также обмен ионов Са²+, фосфора и активность щелочной фосфатазы в плазме крови. Процесс заживления перелома был одинаков по временным показателям у крыс II и III групп, которым в месте перелома вводилась плазма обогащенная тромбоцитами и проводился фонофорез с фиксированной дозой яда. По характеру процесса образования костной мозоли они отличались. Так в I и III группах процесс происходил по остеогенному типу, а во II группе по хондрогенному. При применении плазмы обогащенной тромбоцитами  активируется щелочная фосфатаза плазмы и снижается содержание фосфора и кальция в ней. Под влиянием фонофареза происходит активация поступления Са²+ в облисть перелома, но в отличие от II-группы этот процесс носит перемежающий характер. Высказано пердположение, что обогащенная плазма и фонофарез с «Апизартноном» способствует более активному поступлению кальция в область перелома, а также способствует активации процессов репарации в популяции остеоцитов. Ключевые слова: перелом малоберцовой кости; плазма обогащенная тромбоцитами; обмен кальция; щелочная фосфатаза; фонофарез

Anti-inflammatory and analgesic propertiesof SS-68 indole derivative

The experiments with rats show that when administered intravenously (5 mg/kg) and intragastrically (5, 10, 15 and 20 mg/kg), SS-68 compound exerts an antiinflammatory effect (AIE) on models of acute inflammation, caused by carrageenin and serotonin, and exerts no AIE in case of edema induced by complete Freund‘s adjuvant (CFA) and histamine, except for intragastric administration at a dose of 20 mg/kg. By its antiphlogogenic effect in case of carrageenin and serotonin edemas, SS-68 is comparable to diclofenac (5, 10 and 15 mg/kg intragastrically) and indomethacin (10 mg kg intragastriccally), and exceeds piroxicam (20 mg/kg intragastrically). By reference to its therapeutic index (TI), in the first case SS-68 exceeds diclofenac, indomethacin and piroxicam 2.2, 15.6 and 5.6 times, and in the second case it exceeds them 1.8, 12.8 and 4.4 times, respectivel

EFFECTIVNESS OF IRON CARBOXYMALTOSATE IN PATIENTS WITH HYPOFERRIC ANEMIA ON THE BACKGROUND OF UTERINE HEMORRHAGES. CLINICAL OBSERVATION

The article analyzes possibilities of hypoferric anemia in patients with abnormal uterine hemorrhages. Pathogenetic mechanisms of iron carboxymaltosate in therapy of this pathology are described. A clinical observation of a girl of a growing age with hypoferric anemia is presented and its clinical effectiveness is proved