18 research outputs found

    Diagnosis of Bartonella spp. infection: study of a bacillary angiomatosis case

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    Several dermatoses are considered idiopathic diseases. Many times lesions such as erythema nodosum or erythema multiformis, for example, cannot have their etiology defined. Human infection caused by Bartonella spp. may determine several clinical syndromic expressions. Starting with a clinically, histologically and ultrastructurally documented bacillary angiomatosis case, a review of medical literature was undertaken to evaluate the available diagnostic criteria regarding infection caused by these agents. Histological evaluations were concluded to be, practically speaking, an important and useful diagnostic method, especially when serology is not available.Várias dermatoses são consideradas idiopáticas. Muitas vezes, afecções como eritema nodoso ou eritema multiforme, por exemplo, não podem ter sua etiologia definida. A infecção humana por Bartonella spp. pode determinar várias expressões clínicas sindrômicas. A partir de um caso de angiomatose bacilar com documentação clínica, histológica e ultra-estrutural, foi feita a revisão da literatura médica para avaliar os critérios diagnósticos disponíveis para a infecção por esses agentes. Conclui-se que a avaliação histológica é, na prática, um importante e útil método diagnóstico, especialmente quando a sorologia não estiver disponível.34935

    Association between psoriasis vulgaris and MHC-DRB, -DQB genes as a contribution to disease diagnosis

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    We analyzed 100 control individuals and 60 patients with psoriasis vulgaris from the population of Campinas, Brazil. Typification of class 11 HLA alleles (HLA-DRB1-5 and -DQB1) was carried out through the DNA/PCR/SSP at medium and high resolution. DNA was extracted through a salting-out procedure: 13 DRB1 alleles, 3 DRB3 alleles, I DRB4 allele, 2 DRB5 alleles, and 5 DQB1 alleles were identified at a medium resolution using the PCR/SSP, and 45 DRB1 alleles were identified at a high resolution in analyzed patients. Results showed associations with psoriasis vulgaris: positive associations HLA-DRB3*02 (p < 0.05, chi(2) = 5.10, RR = 2.14); HLA-DRB1*0102 alleles (p < 0.05, RR = 5.44). Negative associations were found for HLA-DRB4*01 (chi(2) = 3.23, RR = 0.55) and HLA-DRB1*1302 alleles (p < 0.05, RR = 0.23). The haplotypes revealed positive association for HLA-DRB1*0102/DQB1*05 (p < 0.05, RR = 5.44) and HLA-DRB1*0701/DQB1*03 alleles (p < 0.02, RR = 9.00). These findings suggest a possible association of the DRB1 allele with the group of patients showing an early onset of the illness, as well as an association with haplotypes HLA-DRB1*0102/DQB1*05 and HLA-DRB1*0701-DQB1*03.15315916

    Ultrastructural changes in a standard strain of Bartonella henselae after passages through BALB/cAn mice

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    Human bartonelloses are a group of illnesses of poorly understood pathogenesis. Bartonella henselae is one of the most studied bacterium of its genus. The objective of this study was to observe whether passages of these bacteria, in vivo, would determine ultrastructural changes in them. For this purpose, isogenic mice were inoculated with a standard strain of B. henselae (I). These were initially retrieved from genetically immunodeficient animals (II) and then inoculated in immunocompetent ones. The bacterial colonies obtained (III) were compared, by transmission electron microscopy, with colonies I and II. Loss of fimbriae and an abundant bleb formation were the most common morphological changes found in colony III. Also, on day 6 postinfection, the main histological abnormalities were the endothelial proliferation presented in immunodeficient animals and the incipient granulomata reaction found in one of the immunocompetent inoculated mice, which died spontaneously. These features agree with the Bartonella human disease clinical and histological observations. This study demonstrates that B. henselae in vivo passages induce significant morphological changes in the bacteria and that these abnormalities could explain their seemingly greater virulence. Most of these observations have not been previously described. Thus, further studies on the Bartonella species pathogenesis should consider these data.26316116

    What Do We (not) Know About The Human Bartonelloses?

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    The human bartonelloses are a group of diseases with a rapidly increasing clinical spectrum. Well known manifestations such as Carrion's disease, trench fever, cat-scratch disease, and bacillary angiomatosis are examples of Bartonella sp. infection. Along with these diseases, recurrent bacteremia, endocarditis, septicemia, erythema nodosum, erythema multiforme, trombocytopenic purpura and other syndromes have been reported having been caused by bacteria of this genus. The infectious process and the pathogenesis of these microorganisms are poorly understood. The bartonelloses may have a benign and self-limited evolution in a host, or a potentially fatal one. These bacteria can provoke a granulomatous or an angioproliferative histopathologic response. As these diseases are not yet well defined, we have reviewed the four main human bartonelloses and have examined unclear points about these emergent diseases.711

    Diagnosis Of Bartonella Spp. Infection: Study Of A Bacillary Angiomatosis Case [diagnóstico Da Infecção Por Bartonella Spp.: A Propósito De Um Caso De Angiomatose Bacilar]

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    Several dermatoses are considered idiopathic diseases. Many times lesions such as erythema nodosum or erythema multiformis, for example, cannot have their etiology defined. Human infection caused by Bartonella spp. may determine several clinical syndromic expressions. Starting with a clinically, histologically and ultrastructurally documented bacillary angiomatosis case, a review of medical literature was undertaken to evaluate the available diagnostic criteria regarding infection caused by these agents. Histological evaluations were concluded to be, practically speaking, an important and useful diagnostic method, especially when serology is not available. ©2006 by Anais Brasileiros de Dermatologia.814349353Velho, P.E.N.F., (2001) Estudo das bartoneloses humanas e da Bartonella henselae: Infecção experimental, microbiologia e microscopia de luz e eletrônica de transmissão, , dissertação, Campinas SP, Faculdade de Ciências Médicas da Universidade Estadual de Campinas;Tappero, J.W., Mohle-Boetani, J., Koehler, J.E., Swaminathan, B., Berger, T.G., Leboit, P.E., The epidemiology of bacillary angiomatosis and bacillary peliosis (1993) J Am Med Assoc, 269, pp. 770-775Koelhler, J.E., Glaser, C.A., Tappero, J.W., Rochalimaea henselae infecion: A new zoonosis with the domestic cat as reservoir (1994) J Am Med Assoc, 71, pp. 531-535Levell, N.J., Bewley, A.P., Chopra, S., Churchill, D., French, P., Miller, R., Bacillary angiomatosis with cutaneous and oral lesions in an HIV-infected patient from the UK (1995) Brit J Dermatol, 132, pp. 113-115Loutit, J.S., Bartonella infections (1997) Cur Clin Top Infec Dis, 17, pp. 269-290Raoult, D., Infections humaines à Bartonella (1999) Presse Med, 28, pp. 429-434Maurin, M., Raoult, D., Bartonella (Rochalimaea) quintana infections (1996) Clin Microbiol Rev, 9, pp. 273-292Garcia-Caceres, U., Garcia, F.U., Bartonellosis: An immunodepressive disease and the life of Daniel Alcides Carrión (1991) Am J Clin Pathol, 95 (SUPPL. 1), pp. S58-S66Brenner, S.A., Rooney, J.A., Manzewitsch, P., Regnery, R.L., Isolation of Bartonella (Rochalimae) henselae: Effects of methods of blood collection and handling (1997) J Clin Microbiol, 35, pp. 544-547Baneth, G., Kordick, D.L., Hergarty, B.C., Breitschwerdt, E.B., Comparative seroreactivity to Bartonella henselae and Bartonella quintana among cats from Israel and North Carolina (1996) Vet Microbiol, 50, pp. 95-103Dehio, C., Sander, A., Bartonella as emerging pathogens (1999) Trends Microbiol, 7, pp. 226-228Regnery, R.L., Rooney, J.A., Johnson, A.M., Nesby, S.L., Manzewitsch, P., Beaver, K., Experimentally induced Bartonella henselae infections followed by challenge exposure and antimicrobial therapy in cats (1996) Am J Vet Res, 57, pp. 1714-1719Velho, P.E.N.F., Moraes, A.M., Uthida-Tanaka, A.M., Cintra, M.L., Gilioli, R., Ultrastructural changes in a standard strain of Bartonella henselae after passages through BALB/cAn mice (2002) Ultrastruct Pathol, 26, pp. 161-169Jensen, W.A., Fall, M.Z., Rooney, J., Kordick, D.L., Breitschwerdt, E.B., Rapid identification and differentiation of Bartonella species using a single-step PCR assay (2000) J Clin Microbiol, 38, pp. 1717-1722Guccion, J.G., Gilbert, C.L., Ortega, L.G., Hadfield, T.L., Cat scratch disease and acquired immunodeficiency disease: Diagnosis by transmission electron microscopy (1996) Ultrastruct Pathol, 20, pp. 195-20
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