Hard X-ray selected giant radio galaxies - I. The X-ray properties and radio connection

We present the first broad-band X-ray study of the nuclei of 14 hard X-ray selected giant radio galaxies, based both on the literature and on the analysis of archival X-ray data from NuSTAR, XMM-Newton, Swift and INTEGRAL. The X-ray properties of the sources are consistent with an accretion-related X-ray emission, likely originating from an X-ray corona coupled to a radiatively efficient accretion flow. We find a correlation between the X-ray luminosity and the radio core luminosity, consistent with that expected for AGNs powered by efficient accretion. In most sources, the luminosity of the radio lobes and the estimated jet power are relatively low compared with the nuclear X-ray emission. This indicates that either the nucleus is more powerful than in the past, consistent with a restarting of the central engine, or that the giant lobes are dimmer due to expansion losses.Comment: 11 pages, 3 figures. Accepted for publication in MNRA

Diseño de un cuestionario de diagnóstico acerca del manejo del concepto de variable en el álgebra

This paper reports the first stage of a project investigating college novice students' interpretations and uses of different realisations of the concept of variable, namely: as unknown, as general number, in functional relationships. In particular, we present and discuss the reliability of a questionnaire that aimed at providing a first students' profile concerning their interpretations and uses of variable. Finally, initial results obtained by analysing students' responses are outlined

An XMM-Newton look at the strongly variable radio-weak BL Lac Fermi J1544-0639

Fermi J1544-0639/ASASSN-17gs/AT2017egv was identified as a gamma-ray/optical transient on May 15, 2017. Subsequent multiwavelength observations suggest that this source may belong to the new class of radio-weak BL Lacs. We studied the X-ray spectral properties and short-term variability of Fermi J1544-0639 to constrain the X-ray continuum emission mechanism of this peculiar source. We present the analysis of an XMM-Newton observation, 56 ks in length, performed on February 21, 2018. The source exhibits strong X-ray variability, both in flux and spectral shape, on timescales of ~10 ks, with a harder-when-brighter behaviour typical of BL Lacs. The X-ray spectrum is nicely described by a variable broken power law, with a break energy of around 2.7 keV consistent with radiative cooling due to Comptonization of broad-line region photons. We find evidence for a `soft excess', nicely described by a blackbody with a temperature of ~0.2 keV, consistent with being produced by bulk Comptonization along the jet.Comment: 11 pages, 12 figures. Accepted for publication in Astronomy & Astrophysic

Expression of a catalytically inactive mutant form of glutathione peroxidase 4 (Gpx4) confers a dominant-negative effect in male fertility.

The selenoenzyme Gpx4 is essential for early embryogenesis and cell viability for its unique function to prevent phospholipid oxidation. Recently, the cytosolic form of Gpx4 was identified as an upstream regulator of a novel form of non-apoptotic cell death, called ferroptosis, whereas the mitochondrial isoform of Gpx4 (mGpx4) was previously shown to be crucial for male fertility. Here, we generated and analyzed mice with targeted mutation of the active site selenocysteine (Sec) of Gpx4 (Gpx4_U46S). Mice homozygous for Gpx4_U46S died at the same embryonic stage (E7.5) as Gpx4-/- embryos as expected. Surprisingly, male mice heterozygous for Gpx4_U46S presented subfertility. Subfertility was manifested in a reduced number of litters from heterozygous breedings and an impairment of spermatozoa to fertilize oocytes in vitro. Morphologically, sperm isolated from heterozygous Gpx4_U46S mice revealed many structural abnormalities particularly in the spermatozoan midpiece due to improper oxidation and polymerization of sperm capsular proteins and malformation of the mitochondrial capsule surrounding and stabilizing sperm mitochondria. These findings are reminiscent of sperm isolated from selenium-deprived rodents or from mice specifically lacking mGpx4. Due to a strongly facilitated incorporation of Ser in the polypeptide chain as compared to Sec at the UGA codon, expression of the catalytically inactive Gpx4_U46S was found to be strongly increased. Since the stability of the mitochondrial capsule of mature spermatozoa depends on the moonlighting function of Gpx4 both as an enzyme oxidizing capsular protein thiols and being a structural protein, tightly controlled expression of functional Gpx4 emerges being key for full male fertility

NEMO regulates a cell death switch in TNF signaling by inhibiting recruitment of RIPK3 to the cell death-inducing complex II

Incontinentia Pigmenti (IP) is a rare X-linked disease characterized by early male lethality and multiple abnormalities in heterozygous females. IP is caused by NF-κB essential modulator (NEMO) mutations. The current mechanistic model suggests that NEMO functions as a crucial component mediating the recruitment of the IκB-kinase (IKK) complex to tumor necrosis factor receptor 1 (TNF-R1), thus allowing activation of the pro-survival NF-κB response. However, recent studies have suggested that gene activation and cell death inhibition are two independent activities of NEMO. Here we describe that cells expressing the IP-associated NEMO-A323P mutant had completely abrogated TNF-induced NF-κB activation, but retained partial antiapoptotic activity and exhibited high sensitivity to death by necroptosis. We found that robust caspase activation in NEMO-deficient cells is concomitant with RIPK3 recruitment to the apoptosis-mediating complex. In contrast, cells expressing the ubiquitin-binding mutant NEMO-A323P did not recruit RIPK3 to complex II, an event that prevented caspase activation. Hence NEMO, independently from NF-κB activation, represents per se a key component in the structural and functional dynamics of the different TNF-R1-induced complexes. Alteration of this process may result in differing cellular outcomes and, consequently, also pathological effects in IP patients with different NEMO mutations

Phospholipid hydroperoxide glutathione peroxidase of rat testis. Gonadotropin dependence and immunocytochemical identification.

A high glutathione peroxidase activity toward phospholipid hydroperoxides is present in rat testis. The attribution of this activity to the selenoenzyme phospholipid hydroperoxide glutathione peroxidase (PHGPX) was supported by cross-reactivity with antibodies raised against pig heart PHGPX which had been purified and characterized. Rat testis PHGPX is partially cytosolic and partially linked to nuclei and mitochondria. The soluble and organelle-bound enzymes appear identical by Western blot analysis. PHGPX, but neither selenium-dependent nor non-selenium-dependent glutathione peroxidase activity, is expressed in testes only after puberty, disappears after hypophysectomy, and is partially restored by gonadotropin treatment. Specific immunostaining of testes by antiserum against PHGPX appears as a fine granular brown pattern localized throughout the cytoplasm in more immature cells but is confined to the peripheral part of the cytoplasm, the nuclear membrane, and mitochondria in maturating spermatogenic cells. As expected, immunostaining of spermatogenic cells in hypophysectomized animals was negative, but gonadotropin treatment only marginally increased the immunoreactivity. The expression of PHGPX in testes is consistent with the previously described specific requirement for selenium for synthesis of a 15-20-kDa selenoprotein which is related to the production of functional spermatozoa

Broadband X-ray spectral analysis of the Seyfert 1 galaxy GRS 1734-292

We discuss the broadband X-ray spectrum of GRS 1734-292 obtained from non-simultaneous XMM-Newton and NuSTAR observations, performed in 2009 and 2014, respectively. GRS1734-292 is a Seyfert 1 galaxy, located near the Galactic plane at $z=0.0214$. The NuSTAR spectrum ($3-80$ keV) is dominated by a primary power-law continuum with $\Gamma=1.65 \pm 0.05$ and a high-energy cutoff $E_c=53^{+11}_{-8}$ keV, one of the lowest measured by NuSTAR in a Seyfert galaxy. Comptonization models show a temperature of the coronal plasma of $kT_e=11.9^{+1.2}_{-0.9}$ keV and an optical depth, assuming a slab geometry, $\tau=2.98^{+0.16}_{-0.19}$ or a similar temperature and $\tau=6.7^{+0.3}_{-0.4}$ assuming a spherical geometry. The 2009 XMM-Newton spectrum is well described by a flatter intrinsic continuum ($\Gamma=1.47^{+0.07}_{-0.03}$) and one absorption line due to Fe\textsc{XXV} K$\alpha$ produced by a warm absorber. Both data sets show a modest iron K$\alpha$ emission line at $6.4$ keV and the associated Compton reflection, due to reprocessing from neutral circumnuclear material

Molecular anatomy of the human glucose 6-phosphate dehydrogenase core promoter

The gene encoding glucose 6-phosphate dehydrogenase (G6PD), which plays a pivotal role in cell defense against oxidative stress, is ubiquitously expressed at widely different levels in various tissues; moreover, G6PD expression is regulated by a number of stimuli. In this study we have analyzed the molecular anatomy of the G6PD core promoter. Our results indicate that the G6PD promoter is more complex than previously assumed; G6PD expression is under the control of several elements that are all required for correct promoter functioning and, furthermore, a still unidentified mammalian specific factor is needed. Copyright (C) 1998 Federation of European Biochemical Societies