PHYTOCHEMICAL AND IN-VITRO ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LACTUCA SCARIOLA & CELOSIA ARGENTEA LEAVES.

Antioxidant compounds in food play an important role as a health protecting factor. Primary sources of naturally occurring antioxidants are whole grains, fruits and vegetables. Highly reactive free radicals and oxygen species can initiate degenerative diseases. Antioxidant compounds like phenolic acids, polyphenols and flavonoids are commonly found in plants have been reported to have multiple biological effects, including antioxidant activity. Methanolic leaf extracts of Lactuca scariola Linn and Celosia argentea Linn were assessed for the In vitro Antioxidant activity using  DPPH (1, 1-diphenyl-2-picryl-hydrazyl) radical  , Nitric oxide(NO) and hydrogen peroxide (H2O2)scavenging models using Ascorbic acid as positive control. Analysis of free radical scavenging activities of the extracts revealed a concentration dependent free radical scavenging activity resulting from reduction in DPPH, Nitric oxide and Hydroxyl radical. The methanolic  extract  of Lactuca scariola Linn showed  significant DPPH , Nitric oxide and hydrogen peroxide scavenging activity compared to Celosia argentea Linn extract . IC50  values by  DPPH , Nitric oxide and hydrogen peroxide model for  Lactuca scariola and  Celosia argentea were found to be 192.5±9.014, 394.2±6.009, 434.2±18.78, 233.3±20.73 , 521.4±4.061and 494.2±5.465 respectively

Some Like it Hot: The X-Ray Emission of The Giant Star YY Mensae

(Abridged abstract) We present an analysis of the X-ray emission of the rapidly rotating giant star YY Mensae observed by Chandra HETGS and XMM-Newton. Although no obvious flare was detected, the X-ray luminosity changed by a factor of two between the XMM-Newton and Chandra observations taken 4 months apart. The coronal abundances and the emission measure distribution have been derived from three different methods using optically thin collisional ionization equilibrium models. The abundances show an inverse first ionization potential (FIP) effect. We further find a high N abundance which we interpret as a signature of material processed in the CNO cycle. The corona is dominated by a very high temperature (20-40 MK) plasma, which places YY Men among the magnetically active stars with the hottest coronae. Lower temperature plasma also coexists, albeit with much lower emission measure. Line broadening is reported, which we interpret as Doppler thermal broadening, although rotational broadening due to X-ray emitting material high above the surface could be present as well. We use two different formalisms to discuss the shape of the emission measure distribution. The first one infers the properties of coronal loops, whereas the second formalism uses flares as a statistical ensemble. We find that most of the loops in the corona of YY Men have their maximum temperature equal to or slightly larger than about 30 MK. We also find that small flares could contribute significantly to the coronal heating in YY Men. Although there is no evidence of flare variability in the X-ray light curves, we argue that YY Men's distance and X-ray brightness does not allow us to detect flares with peak luminosities Lx <= 10^{31} erg/s with current detectors.Comment: Accepted paper to appear in Astrophysical Journal, issue Nov 10, 2004 (v615). This a revised version. Small typos are corrected. Figure 7 and its caption and some related text in Sct 7.2 are changed, without incidence for the conclusion

Composition change-driven texturing and doping in solution-processed SnSe thermoelectric thin films

The discovery of SnSe single crystals with record high thermoelectric efficiency along the b-axis has led to the search for ways to synthesize polycrystalline SnSe with similar efficiencies. However, due to weak texturing and difficulties in doping, such high thermoelectric efficiencies have not been realized in polycrystals or thin films. Here, we show that highly textured and hole doped SnSe thin films with thermoelectric power factors at the single crystal level can be prepared by solution process. Purification step in the synthetic process produced a SnSe-based chalcogenidometallate precursor, which decomposes to form the SnSe2 phase. We show that the strong textures of the thin films in the b???c plane originate from the transition of two dimensional SnSe2 to SnSe. This composition change-driven transition offers wide control over composition and doping of the thin films. Our optimum SnSe thin films exhibit a thermoelectric power factor of 4.27 ??W cm???1 K???2

Curation of viral genomes: challenges, applications and the way forward

BACKGROUND: Whole genome sequence data is a step towards generating the 'parts list' of life to understand the underlying principles of Biocomplexity. Genome sequencing initiatives of human and model organisms are targeted efforts towards understanding principles of evolution with an application envisaged to improve human health. These efforts culminated in the development of dedicated resources. Whereas a large number of viral genomes have been sequenced by groups or individuals with an interest to study antigenic variation amongst strains and species. These independent efforts enabled viruses to attain the status of 'best-represented taxa' with the highest number of genomes. However, due to lack of concerted efforts, viral genomic sequences merely remained as entries in the public repositories until recently. RESULTS: VirGen is a curated resource of viral genomes and their analyses. Since its first release, it has grown both in terms of coverage of viral families and development of new modules for annotation and analysis. The current release (2.0) includes data for twenty-five families with broad host range as against eight in the first release. The taxonomic description of viruses in VirGen is in accordance with the ICTV nomenclature. A well-characterised strain is identified as a 'representative entry' for every viral species. This non-redundant dataset is used for subsequent annotation and analyses using sequenced-based Bioinformatics approaches. VirGen archives precomputed data on genome and proteome comparisons. A new data module that provides structures of viral proteins available in PDB has been incorporated recently. One of the unique features of VirGen is predicted conformational and sequential epitopes of known antigenic proteins using in-house developed algorithms, a step towards reverse vaccinology. CONCLUSION: Structured organization of genomic data facilitates use of data mining tools, which provides opportunities for knowledge discovery. One of the approaches to achieve this goal is to carry out functional annotations using comparative genomics. VirGen, a comprehensive viral genome resource that serves as an annotation and analysis pipeline has been developed for the curation of public domain viral genome data . Various steps in the curation and annotation of the genomic data and applications of the value-added derived data are substantiated with case studies

Increased risk of severe clinical course of COVID-19 in carriers of HLA-C*04:01

Background: Since the beginning of the coronavirus disease 2019 (COVID-19) pandemic, there has been increasing urgency to identify pathophysiological characteristics leading to severe clinical course in patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Human leukocyte antigen alleles (HLA) have been suggested as potential genetic host factors that affect individual immune response to SARS-CoV-2. We sought to evaluate this hypothesis by conducting a multicenter study using HLA sequencing. Methods: We analyzed the association between COVID-19 severity and HLAs in 435 individuals from Germany (n = 135), Spain (n = 133), Switzerland (n = 20) and the United States (n = 147), who had been enrolled from March 2020 to August 2020. This study included patients older than 18 years, diagnosed with COVID19 and representing the full spectrum of the disease. Finally, we tested our results by meta-analysing data from prior genome-wide association studies (GWAS). Findings: We describe a potential association of HLA-C*04:01 with severe clinical course of COVID-19. Carriers of HLA-C*04:01 had twice the risk of intubation when infected with SARS-CoV-2 (risk ratio 1.5 [95% CI 1.1-2.1], odds ratio 3.5 [95% CI 1.9-6.6], adjusted p-value = 0.0074). These findings are based on data from four countries and corroborated by independent results from GWAS. Our findings are biologically plausible, as HLA-C*04:01 has fewer predicted bindings sites for relevant SARS-CoV-2 peptides compared to other HLA alleles. Interpretation: HLA-C*04:01 carrier state is associated with severe clinical course in SARS-CoV-2. Our findings suggest that HLA class I alleles have a relevant role in immune defense against SARS-CoV-2. Funding: Funded by Roche Sequencing Solutions, Inc