The Impact of Teachers’ Communication Styles on Pupils’ Self- safety Throughout the Learning Process

AbstractOver the last decade, numerous research studies have revealed the multiple roles a teacher must implement: trainer, communicator, developer and shaper of students’ personalities, counsellor, and provider of secure learning environments for students, etc. The whole range of such roles is sustained by personality features.Taking into account preadolescents’ main characteristics, this paper attempts to reveal the impact that a teacher has upon students’ safety in the learning process in terms of his/her unique communication style

Molecular cloning using in vivo DNA assembly

Here we describe the in vivo DNA assembly approach, where molecular cloning procedures are performed using an E. coli recA-independent recombination pathway, which assembles linear fragments of DNA with short homologous termini. This pathway is present in all standard laboratory E. coli strains and, by bypassing the need for in vitro DNA assembly, allows simplified molecular cloning to be performed without the plasmid instability issues associated with specialized recombination-cloning bacterial strains. The methodology requires specific primer design and can perform all standard plasmid modifications (insertions, deletions, mutagenesis, and sub-cloning) in a rapid, simple, and cost-efficient manner, as it does not require commercial kits or specialized bacterial strains. Additionally, this approach can be used to perform complex procedures such as multiple modifications to a plasmid, as up to 6 linear fragments can be assembled in vivo by this recombination pathway. Procedures generally require less than 3 h, involving PCR amplification, DpnI digestion of template DNA, and transformation, upon which circular plasmids are assembled. In this chapter we describe the requirements, procedure, and potential pitfalls when using this technique, as well as protocol variations to overcome the most common issues

LITERATURA ORAL Y CULTURA POPULAR DE LA COMARCA DE LA SIERRA DE SEGURA (JAÉN)

En esta tesis se transcriben y estudian 523 textos orales documentados en la comarca jiennense de la Sierra de Segura, zona que apenas había sido estudiada hasta la fecha. Para el análisis de las versiones recopiladas se tienen en cuenta varios repertorios, investigaciones y archivos digitales como el Corpus de Literatura Oral (CLO) y el Fondo de Música Tradicional IMF-CSIC (FMT). Con este trabajo, se espera contribuir al conocimiento y la difusión de este importante patrimonio cultural, que nos hemos ocupado de estudiar desde el punto de vista de los temas y estructuras literarias, además de los contextos en los que estos registros se han transmitido en sus comunidades originarias. En cuanto a los géneros considerados en nuestro corpus oral, ofrecemos versiones pertenecientes al romancero, cancionero (incluidas las canciones del repertorio infantil), cuentos y leyendas, además de textos oralizados y de autor y otros testimonios relacionados con la historia oral.This thesis dissertation transcribes and studies 523 oral texts and documented in Sierra de Segura region (province of Jaen, Spain), where there is a lack of studies to date. It has been taken into account several repertoires, investigations and files for the analysis of the compiled versions, such as Corpus Literatura Oral (Oral Literature Corpus) and TMH (traditional Music Holdings). With the present study, we aim to contribute to the knowledge and the promotion of this vital cultural heritage, in addition to those contexts where these registers were transmitted to their native communities. Regarding the analyzed contexts in our oral corpus, we offer different versions from ballad songs, anthology of songs (including child songs), tales, legends and oral author texts, among other testimonies related with oral history.Tesis Univ. Jaén. Departamento de Filología Española. Leída el 11 de mayo de 2020

Desarrollo de un sistema robótico para el reparto personalizado de manos de contract bridge

Este Proyecto fin de Carrera ha consistido en el estudio, diseño y construcción de un sistema robótico que es capaz de repartir manos de cartas personalizadas para el juego del Contract Bridge. El sistema robótico constará de dos partes: una parte hardware, que atenderá al diseño y construcción del sistema robótico en sí, y de una parte software, que será capaz de controlar el robot, y de presentar un interfaz para definir la mano que se desea repartir.This thesis consists on the study, design and build a suitable robotic system to distribute custom hands for the game of Bridge cards. The robotic system has two parts: software, programming language for sending commands to the robotic system; and hardware part will consist of the design and construction of the system, with its conclusions to an appropriate design

Structural insights into promiscuous GPCR-G protein coupling

G protein-coupled receptors (GPCRs) transduce extracellular signals across biological membranes by activating heterotrimeric Gαβγ proteins. There are 16 different human Gα proteins grouped into four families (GS, Gi/O, Gq/11 and G12/13), each one activating different signaling cascades. Around 50% of non-olfactory GPCRs activate more than one type of Gα proteins with different efficacy and kinetics, triggering a fingerprint-like signaling profile. In this chapter we review the GPCR-G protein promiscuity landscape and discuss recent structures of GPCRs coupled to different Gα proteins. Overall, the size and shape of the intracellular cavity (determined by the extent of outward movement of TM6) is maintained when the receptor is coupled to different Gα proteins, and is determined by the type of primary Gα coupling. The “sub-optimal” secondary Gα coupling is further supported by interactions with the intracellular loops, with ICL2 and ICL3 having a relevant role in promiscuous couplings

Estudios sobre la activación de los receptores de dopamina

Debido a la gran homología de secuencia en el sitio ortostérico de los receptores de dopamina, los fármacos anti-Parkinson no son muy específicos entre los receptores, lo que produce efectos secundarios. Por este motivo, se ha intentado medir la afinidad de fármacos por los receptores, para estudiar los más específicos y desarrollar fármacos más efectivos. Sin embargo, diferentes laboratorios han reportado diferentes afinidades y no se ha llegado a un consenso.En este trabajo se pretende poner a punto una técnica para medir la afinidad de fármacos anti-Parkinson por los receptores de dopamina de manera directa (para sobrepasar los problemas con las técnicas actuales) y utilizarla para comprobar si la mutación S9G del receptor D3R modifica la afinidad de los fármacos, así como obtener el receptor D4R funcional in vitro para futuros estudios estructurales.Aunque la técnica utilizada ha mostrado ser fiable para medir la termoestabilidad de los receptores de dopamina, en el estado actual no es adecuada para ser utilizada en la medida de la afinidad de fármacos anti-Parkinson por receptores, pues es poco reproducible. Por otra parte, no se ha podido estudiar el mutante D3R S9G por problemas de contaminaciones en los cultivos de células que expresaban el receptor, que han impedido su obtención en solución.Con respecto al D4R, se han ensayado diferentes condiciones de solubilización del receptor y se ha encontrado que las condiciones en las que el receptor es más estable en detergente LMNG + CHS sin proteínas G, y que además los fármacos no modifican su estabilidad.<br /

The endoplasmic reticulum Ca2+-ATPase SERCA2b is upregulated in activated microglia and its inhibition causes opposite effects on migration and phagocytosis

This is the peer reviewed version of the following article: Morales-Ropero JM, Arroyo-Urea S, Neubrand VE, Martín-Oliva D, Marín-Teva JL, Cuadros MA, Vangheluwe P, Navascués J, Mata AM, Sepúlveda MR. The endoplasmic reticulum Ca2+ -ATPase SERCA2b is upregulated in activated microglia and its inhibition causes opposite effects on migration and phagocytosis. Glia. 2021 Apr;69(4):842-857, which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1002/glia.23931. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.The accepted version is under embargo until April 2022.Activation of microglia is an early immune response to damage in the brain. Although a key role for Ca2+ as trigger of microglial activation has been considered, little is known about the molecular scenario for regulating Ca2+ homeostasis in these cells. Taking into account the importance of the endoplasmic reticulum as a cellular Ca2+ store, the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA2b) is an interesting target to modulate intracellular Ca2+ dynamics. We found upregulation of SERCA2b in activated microglia of human brain with Alzheimer´s disease and we further studied the participation of SERCA2b in microglial functions by using the BV2 murine microglial cell line and primary microglia isolated from mouse brain. To trigger microglia activation, we used the bacterial lipopolysaccharide (LPS), which is known to induce an increase of cytosolic Ca2+. Our results showed an upregulated expression of SERCA2b in LPS-induced activated microglia likely associated to an attempt to restore the increased cytosolic Ca2+ concentration. We analyzed SERCA2b contribution in microglial migration by using the specific SERCA inhibitor thapsigargin in scratch assays. Microglial migration was strongly stimulated with thapsigargin, even more than with LPS-induction, but delayed in time. However, phagocytic capacity of microglia was blocked in the presence of the SERCA inhibitor, indicating the importance of a tight control of cytosolic Ca2+ in these processes. All together, these results provide for the first time compelling evidence for SERCA2b as a major player regulating microglial functions, affecting migration and phagocytosis in an opposite manner.Grant mP_BS_35-2014 from CEI BioTic GranadaPP2016-PJI05 from University of GranadaA1-CTS-324-UGR18 from FEDER-Junta de Andalucía, SpainPP2016-PIP08 from University of GranadaBFU2017-85723-P from Spanish Ministry of Economy and Competitiveness co-financed with FEDERG044212N from Flanders Research Foundatio